Fa'atonu RT-qPCR fa'asologa
Fa'atonu PCR
O le a le Direct PCR
Direct PCR o le auala o le faʻalauteleina o le DNA saʻo mai le toto, aano o manu, siʻusiʻu isumu, sela, taliga isumu, iʻa zebra, fuamoa iʻa, lau laau, fatu poʻo isi faʻataʻitaʻiga o mea ola e aunoa ma le faia o DNA tuʻuesea ma faʻamamāina laasaga.E mafai ona fa'aititia tele le taimi fa'ata'ita'i, ma le tau i fa'asologa fa'asolosolo ma galuega tetele.E maua ai fo'i se filifiliga sili atu pe a feagai ma lu'itau o le fa'ateleina o se vaega la'ititi o fa'ata'ita'iga lea e mafai ai e le la'asaga fa'amamāina ona ta'ita'i atu ai i fa'ata'ita'iga leiloa.
FOREGENE's PCR Mix e malosi le fa'alavelave fa'alavelave, ona o le malosi FOREGENE pateni Taq enzyme (pateni fa'atagaina: ZL20161034512.0 (Saina), EP3450560 (Europa), PCT/CN2017/082154 (US), pateni 6894926 (Iapani)).O lona Taq enzyme e fa'aogaina ai le DNA recombination technology e toe tu'ufa'atasia ai le DNA binding structure region o le archaeal nucleic acid binding protein ma le vevela-resistant Taq DNA polymerase e fausia ai se DNA fa'ata'ita'i ma fa'ateleina le fa'atasi.O se Taq fa'alelei vevela-amata o lo'o taofia ai galuega eseese o le DNA polymerase muamua. O lenei Taq enzyme e mafai ona fusifusia i DNA fa'ata'ita'i ma amata ai le fa'atupuina o le DNA i fa'ata'ita'iga maualalo fa'atonu ma si'osi'omaga lavelave.
Mo se faʻataʻitaʻiga, e faigofie e pei o le faʻaopopoina o lau sela, laau (laʻau laʻau, aʻa poʻo fatu) poʻo manu faʻataʻitaʻiga i le paʻu, ma faʻaopoopo le faʻafefiloi faʻapitoa faʻatasi ma faʻamaufaʻailoga faʻapitoa i paipa PCR ma faʻaogaina e ala i se uila vevela.O le fa'aogaina o pusa e lelei tele mo su'esu'ega fa'ata'ita'iga lapopo'a lea e taua tele ai le fa'asaoina o le taimi.
O le PCR reaction system o loʻo i ai le enzyme UNG ma le dUTP ua filifilia e tusa ai ma faʻataʻitaʻiga manaʻoga, lea e mafai ona aloese mai le faʻaleagaina e mafua mai i oloa faʻalauteleina PCR.
Tulaga lelei oDPCR tuusao
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| Fa'atonu PCR | Auala masani (RNA fa'amamāina + masani PCR) |
| Fa'aaogāga meafaitino | Fa'aaogāina o le tino | itiiti | Tele |
| Sauniuniga mamanu | Ituaiga meafaitino | Leai se olo e mana'omia | Omea fa'ata'ita'iga |
| Fa'agaioiga | Tulaga tasi-tulaga, 10min | fa'alavelave lavelave, 1h | |
| Fulu | 1-96 | 1-24 | |
| tali PCR | faiga tali | Fa'aopoopoina 2×PCR fa'afefiloi | Dntp, MgCl2,10×PCR Pa'u,Taq enzyme, |
Atalosaga
01 Fa'ailoaina o meaola ninii fa'ama'i
02 Fa'ailoga fa'avasegaina, fa'ailoga fa'a-genotype
03 Multiplex PCR / SNP su'esu'ega / PCR-RFLP
04 Fa'asologa/cloning
Fa'asologa o oloa
| Fa'asologa | Igoa Oloa | Fa'amatalaga | Numera Fa'amaumauga | Tulaga Teuteu |
| Fa'atonu RT-qPCR Fa'asologa | 200T | DRT-01011 | Vaega I 4℃, Vaega II -20℃ | |
| 1000T | DRT-01012 |
| Fa'asologa | Igoa Oloa | Fa'amatalaga | Numera Fa'amaumauga | Tulaga Teuteu |
|
Toto Direct PCR Fa'asologa | 200 × 20μl rxns | TP-04111 | -20℃ | |
| 2000 × 20μl rxns | TP-04113 | |||
| 200 × 20μl rxns | TP-04121 | -20℃ | ||
| 2000 × 20μl rxns | TP-04123 | |||
| 200 × 20μl rxns | TP-04211 | -20℃ | ||
| 2000 × 20μl rxns | TP-04213 | |||
| 200 × 20μl rxns | TP-04221 | -20℃ | ||
| 2000 × 20μl rxns | TP-04223 |
| Series | Igoa Oloa | Fa'amatalaga | Numera Fa'amaumauga | Tulaga Teuteu |
| Manu Direct PCR Fa'asologa | Pusa PCR sa'o Tissue Manu(65℃ lysis) | 200 × 20μl rxns | TP-01111 | Vaega I 4℃, Vaega II -20℃ |
| 2000 × 20μl rxns | TP-01113 | |||
| Pusa PCR sa'o Tissue Manu(65℃ lysis) -UNG | 200 × 20μl rxns | TP-01121 | Vaega I 4℃, Vaega II -20℃ | |
| 2000 × 20μl rxns | TP-01123 | |||
| Isu Si'u SuperDirectTMPusa PCR (lisi o le vevela o le potu) | 200 × 20μl rxns | TP-01311 | Vaega I 4℃, Vaega II -20℃ | |
| 2000 × 20μl rxns | TP-01313 | |||
| Pusa PCR Tu'usa'o Si'u Isu(65℃ lysis) | 200 × 20μl rxns | TP-01331 | Vaega I 4℃, Vaega II -20℃ | |
| 2000 × 20μl rxns | TP-01333 | |||
| Pusa PCR Tu'usa'o Si'u Isu(65℃ lysis) - UNG | 200 × 20μl rxns | TP-01341 | Vaega I 4℃, Vaega II -20℃ | |
| 2000 × 20μl rxns | TP-01343 | |||
| Sebra Fish Direct PCR Kit(65℃ lysis) | 200 × 20μl rxns | TP-01411 | Vaega I 4℃, Vaega II -20℃ | |
| 2000 × 20μl rxns | TP-01413 | |||
| Sebra Fish Direct PCR Kit(65℃ lysis) - UNG | 200 × 20μl rxns | TP-01421 | Vaega I 4℃, Vaega II -20℃ | |
| 2000 × 20μl rxns | TP-01423 |
| Fa'asologa | Igoa Oloa | Fa'amatalaga | Numera Fa'amaumauga | Tulaga Teuteu |
| La'au Tu'usa'o PCR Fa'asologa | 200 × 20μl rxns | TP-02111 | Vaega I 4℃, Vaega II -20℃ | |
| 2000 × 20μl rxns | TP-02113 | |||
| 200 × 20μl rxns | TP-02121 | Vaega I 4℃, Vaega II -20℃ | ||
| 2000 × 20μl rxns | TP-02123 | |||
| La'au La'au Tu'usa'o PCR Plus Kit | 200 × 20μl rxns | TP-02131 | Vaega I 4℃, Vaega II -20℃ | |
| 2000 × 20μl rxns | TP-02133 | |||
| La'au La'au Tu'usa'o PCR Plus Kit -UNG | 200 × 20μl rxns | TP-02141 | Vaega I 4℃, Vaega II -20℃ | |
| 2000 × 20μl rxns | TP-02143 | |||
| 200 × 20μl rxns | TP-03111 | Vaega I 4℃, Vaega II -20℃ | ||
| 2000 × 20μl rxns | TP-03113 | |||
| 200 × 20μl rxns | TP-03121 | Vaega I 4℃, Vaega II -20℃ | ||
| 2000 × 20μl rxns | TP-03123 | |||
| 200 × 20μl rxns | TP-03131 | Vaega I 4℃, Vaega II -20℃ | ||
| 2000 × 20μl rxns | TP-03133 | |||
| 200 × 20μl rxns | TP-03141 | Vaega I 4℃, Vaega II -20℃ | ||
| 2000 × 20μl rxns | TP-03143 | |||
| Fuga La'au Tu'usa'o PCR Plus Kit I(Polysaccharide polyphenol laau) | 200 × 20μl rxns | TP-03151 | Vaega I 4℃, Vaega II -20℃ | |
| 2000 × 20μl rxns | TP-03153 | |||
| Fua Fa'ato'a Tu'usa'o PCR Plus Kit I - UNG(Polysaccharide polyphenol laau) | 200 × 20μl rxns | TP-03161 | Vaega I 4℃, Vaega II -20℃ | |
| 2000 × 20μl rxns | TP-03163 | |||
| 200 × 20μl rxns | TP-03171 | Vaega I 4℃, Vaega II -20℃ | ||
| 2000 × 20μl rxns | TP-03173 | |||
| Fua Fa'ato'a Tu'usa'o PCR Plus Kit II - UNG(Polysaccharide polyphenol plant) | 200 × 20μl rxns | TP-03181 | Vaega I 4℃, Vaega II -20℃ | |
| 2000 × 20μl rxns | TP-03183 |
