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Pusa PCR Tu'usa'o I/II (e aunoa ma ni Meafaigaluega Fa'ata'ita'i) Polokalama Pusa PCR Tu'usa'o La'au.

Fa'amatalaga o pusa:

Leai se taimi faʻaalu ma taugata faʻamamaina DNA (Taimi, galuega ma tau faʻasaoina!)

itiiti meafaitino

Faigofie-o le faʻataʻitaʻiga e mafai ona tuʻuina atu i le PCR reaction poʻo le lysis reaction e aunoa ma le tipiina pe olo

2 X Faafefiloi—faaitiitia o mea sese o le utaina o faataitaiga ma le taimi o sauniuniga faiga faiga

Anapogi—o le tapenaga o ata e mafai ona mae'a ile 10 minute, ole PCR e mafai ona mae'a ile 50 minute

E mafai ona fa'amae'aina le fa'aogaina o le lysis i le 96-well PCR plate

malosi mua'i


Fa'amatalaga Oloa

Faailoga o oloa

Fa'amatalaga

O lenei oloa e fa'aogaina ai le fa'aogaina o le lysis buffer system e fa'amama ai fatu laau.O le lysate e mafai ona faʻaaogaina e fai ma faʻataʻitaʻiga e aunoa ma le faʻamamaina.A maeʻa ona faʻaopoopo mea muamua, o le PCR Mix o lenei pusa e mafai ona faʻaogaina mo le faʻalauteleina.

Fa'amatalaga

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Vaega o pusa

Pusa PCR Tu'usa'o Fatu Toto I

Vaega I

Puipui SP1

Puipui SP2

6 × DNA Loading Buffer

Vaega II

2×Seed PCR FaigofieTMFaafefiloi


Pusa PCR Tu'usa'o o Fatu Toto II

Vaega I

Puipui SP1

Puipui SP2

6 × DNA Loading Buffer

Vaega II

2×Seed PCR FaigofieTMFaafefiloi

Fa'apitoa & itu lelei

■ Leai se taimi e alu ai ma taugata DNA fa'amamāina

■ Laiti meafaitino

■ Faigofiele faʻataʻitaʻiga e mafai ona tuʻuina atu i le PCR reaction poʻo le lysis reaction e aunoa ma le tipiina poʻo le olo

■ 2X Faafefiloifaʻaitiitia faʻataʻitaʻiga uta mea sese ma tali faiga taimi sauniuni

■ Anapogie mafai ona maeʻa sauniuniga faʻataʻitaʻiga i le 10 minute, e mafai ona maeʻa le tali PCR ile 50 minute

■ Mauaina maualugae mafai ona faamaeaina tali lysis i 96-well ipu PCR.

Fa'asologa o pusa

Fa'aoga: Fa'ailoaina o la'au transgenic, genotyping, etc.

Fa'ata'ita'iga: Toto fatu po'o a'au e maualalo le polysaccharide ma le polyphenol.

Fa'ata'ita'iga tele: fatu tasi atoa, 1-100mg aano o la'au

Su'esu'ega: PCR oloa ≤1kb

Fa'agasologa o galuega

toto fatu tuusao PCR

Electropherogram

toto fatu tuusao PCR-DIAGRAM

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