Pusa PCR Tu'usa'o I/II (e aunoa ma ni Meafaigaluega Fa'ata'ita'i) Polokalama Pusa PCR Tu'usa'o La'au.
Fa'amatalaga
O lenei oloa e fa'aogaina ai le fa'aogaina o le lysis buffer system e fa'amama ai fatu laau.O le lysate e mafai ona faʻaaogaina e fai ma faʻataʻitaʻiga e aunoa ma le faʻamamaina.A maeʻa ona faʻaopoopo mea muamua, o le PCR Mix o lenei pusa e mafai ona faʻaogaina mo le faʻalauteleina.
Fa'amatalaga
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Vaega o pusa
Pusa PCR Tu'usa'o Fatu Toto I | |
Vaega I | Puipui SP1 |
Puipui SP2 | |
6 × DNA Loading Buffer | |
Vaega II | 2×Seed PCR FaigofieTMFaafefiloi |
Pusa PCR Tu'usa'o o Fatu Toto II | |
Vaega I | Puipui SP1 |
Puipui SP2 | |
6 × DNA Loading Buffer | |
Vaega II | 2×Seed PCR FaigofieTMFaafefiloi |
Fa'apitoa & itu lelei
■ Leai se taimi e alu ai ma taugata DNA fa'amamāina
■ Laiti meafaitino
■ Faigofie—le faʻataʻitaʻiga e mafai ona tuʻuina atu i le PCR reaction poʻo le lysis reaction e aunoa ma le tipiina poʻo le olo
■ 2X Faafefiloi—faʻaitiitia faʻataʻitaʻiga uta mea sese ma tali faiga taimi sauniuni
■ Anapogi—e mafai ona maeʻa sauniuniga faʻataʻitaʻiga i le 10 minute, e mafai ona maeʻa le tali PCR ile 50 minute
■ Mauaina maualuga—e mafai ona faamaeaina tali lysis i 96-well ipu PCR.
Fa'asologa o pusa
Fa'aoga: Fa'ailoaina o la'au transgenic, genotyping, etc.
Fa'ata'ita'iga: Toto fatu po'o a'au e maualalo le polysaccharide ma le polyphenol.
Fa'ata'ita'iga tele: fatu tasi atoa, 1-100mg aano o la'au
Su'esu'ega: PCR oloa ≤1kb