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Fatu La'au (Polysaccharide Polyphenol mauoa, lapopo'a ma feololo) Direct PCR Plus Kit II-UNG(e aunoa ma ni Meafaigaluega Fa'ata'ita'i)

Fatu La'au (Polysaccharide Polyphenol mauoa, lapopo'a ma feololo) Direct PCR Plus Kit II-UNG(e aunoa ma ni Meafaigaluega Fa'ata'ita'iga) Ata Fa'aalia
  • Fatu La'au (Polysaccharide Polyphenol mauoa, lapopo'a ma feololo) Direct PCR Plus Kit II-UNG(e aunoa ma ni Meafaigaluega Fa'ata'ita'i)
  • Fatu La'au (Polysaccharide Polyphenol mauoa, lapopo'a ma feololo) Direct PCR Plus Kit II-UNG(e aunoa ma ni Meafaigaluega Fa'ata'ita'i)
  • Fatu La'au (Polysaccharide Polyphenol mauoa, lapopo'a ma feololo) Direct PCR Plus Kit II-UNG(e aunoa ma ni Meafaigaluega Fa'ata'ita'i)
  • Fatu La'au (Polysaccharide Polyphenol mauoa, lapopo'a ma feololo) Direct PCR Plus Kit II-UNG(e aunoa ma ni Meafaigaluega Fa'ata'ita'i)

Fa'amatalaga o pusa:

Fa'aaoga sa'o le polysaccharide polyphenol seed lysate e fai ma fa'ata'ita'iga mo le fa'alauteleina o le PCR, e aunoa ma le fa'aesea o le DNA, vave ma maualuga le lagona.O le UNG anti-pollution system ua faʻaopoopoina e puipuia ai mea sese.

◮E leai se taimi e alu ai ma taugata DNA e manaʻomia.

◮O le faʻataʻitaʻiga manaʻomia e laʻititi, naʻo le ave o le fatu e tasi.

◮E leai ni togafitiga faʻapitoa e pei o le oloina ma le tuʻimomomoina e manaʻomia, ma o le taotoga e faigofie.

◮Ole faiga ole PCR sili ona lelei e mafai ai e le PCR ona maua le fa'apitoa maualuga ma le fa'apalepale malosi atu ile iniseti tali ole PCR.

malosi mua'i


Fa'amatalaga Oloa

Faailoga o oloa

Fa'amatalaga

O lenei oloa o loʻo faʻaaogaina se faiga faʻapitoa lysis reaction system, lea e mafai ona vave tuʻuina atu genomic DNA mai faʻataʻitaʻiga fatu laau ma polysaccharides maualuga ma polyphenols (e pei o faʻi, cotton, ma isi) mo le faʻaogaina i PCR reactions, o lea e fetaui lelei mo suʻesuʻega faʻatupu tele.Ina ia fa'amalieina mana'oga o su'ega su'esu'ega 'ese'ese, e mafai e lenei pusa ona fa'aogaina le tele o ituaiga fa'ata'ita'iga (e pei o fatu atoa, tipi'i laiti, po'o fa'ata'ita'iga eleele o fatu e tele) mo su'ega.Faatasi ai ma i latou, mo faʻataʻitaʻiga fatu o loʻo manaʻomia pea ona faʻatupuina, e mafai ona e filifili e tipi ese ni fasipisi micro-tissue (1-5mg) e ese mai i fatu embryos mo suʻega;mo faʻataʻitaʻiga e manaʻomia ai faʻataʻitaʻiga ma suʻega i se numera tele o faʻataʻitaʻiga, e mafai ona e filifili e faʻafefiloi le tele o faʻataʻitaʻiga ma tuʻufaʻatasia.A maeʻa ona oloina i ni vaega e tusa ma le 0.5mm le lautele, e mafai ona faia le faʻataʻitaʻiga (a itiiti ifo i le 0.1% o le faʻataʻitaʻiga e mafai ona iloa).

Fa'amatalaga

50T,200T,500T,2000T

Vaega o pusa

Fua Toto Tu'usa'o PCR Plus Kit II

Polysaccharide polyphenol fatu laau (lapopoa ma feololo) pusa PCR tuusao

 Fausia pusa

 TP-0317T TP-03171

TP-03172

 TP-03173
50 T 200 T 500 T 2000 T
 

Vaega I

 Puipui SSP1 24ml 96ml

120ml×2

 480ml×2
Puipui SP2 3ml 10ml 25ml 100ml
6x DNA Loading Buffer 1.5ml 1.5ml 1.5ml 1.5ml×4
 

Vaega II

 Puipui SSP2 8ml 32ml 80ml 80ml×4
2× Seed PCR FaigofieTMFaafefiloi 500μl 1ml×2

1.7ml×3

 1.7ml×12
Tusi Lesona 1 1 1 1

Fa'ailoga ma mea lelei

- E leai se taimi e alu ai ma taugata DNA e manaʻomia.

- E laʻititi le manaʻoga faʻataʻitaʻi, naʻo le ave o le fatu e tasi.

- E leai ni togafitiga faʻapitoa e pei o le oloina ma le tuʻimomomoina e manaʻomia, ma e faigofie le taʻaloga.

- O le faiga o le PCR sili ona lelei e mafai ai e le PCR ona maua le maualuga maualuga ma le malosi o le faapalepale i le PCR reaction inhibitors.

Fa'asologa o galuega

Fua Fa'ato'a Tu'usa'o PCR Plus Kit I(Laiti)-UNG

Tulaga teuina

1. Felauaiga

O le faagasologa atoa o felauaiga pusa aisa maualalo-vevela, ina ia mautinoa o le pusa Vaega II o loʻo i se setete o <4 ℃.

2. Teuga

Vaega I o lenei pusa e tatau ona teuina i le vevela o le potu poʻo le 2-8°C.

Vaega II e tatau ona teuina ile -20°C.

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