Toto SuperDirectᵀᴹ PCR Kit-EDTA Toto Direct PCR Master Mix mo le Genotyping o le Toto.
Fa'amatalaga
200 × 20μl rxns, 2000 × 20μl rxns
2× SuperEasyTMFaafefiloi-EDTA: O loʻo i ai le D-Taq DNA Polymerase faʻapitoa, dNTPs, MgCl2, faʻafefe tali, PCR reaction enhancer, optimizer ma stabilizer, ma isi.2O i le tutusa 2× SuperEasyTMFa'afefiloi-EDTA e fa'aoga ile tali ole PCR.
6× DNA Loading Buffer: O le Loading Buffer e le o iai SDS.E fautuaina e faʻaoga le 6 × DNA Loading Buffer e aofia ai ma le pusa pe a faʻatino le electrophoresis gel agarose ina ia maua ai faʻaiʻuga lelei electrophoresis.Aua le faʻaogaina le Loading Buffer o loʻo i ai le SDS, a leai o le ai ai se siʻusiʻu tele o le malamalama susulu i le auala i le taimi o le electrophoresis, lea o le a aʻafia ai iʻuga faʻataʻitaʻiga.
Vaega o pusa
2× SuperEasyTMFaafefiloi-EDTA |
6x DNA Loading Buffer |
Faatonuga |
Fa'apitoa & itu lelei
-E leai se manaʻoga mo le faʻaaluina o le taimi ma taugata DNA faʻamamaina, leai se togafitiga muamua, ma le faʻaogaina saʻo o le toto atoa o se PCR template.
-O le faiga o loʻo i ai le malosi faʻamalosia malosi ma e mafai ona iloa maʻaleʻale le genome ma faʻailoga i fafo atu o DNA i totonu o le toto.
-2× Fa'afaigofieTMMix-EDTA Fa'apalepale toto maualuga: e o'o atu i le 45% ile toto o le tagata ma le 20% ile toto isumu.
-O le faʻataʻitaʻiga o loʻo faʻapipiʻiina atoa, o lea e leai se manaʻoga e popole e uiga i faʻataʻitaʻiga faʻaleagaina ma taunuʻuga PCR lelei sese.
Fa'aoga pusa
Fa'apitoa mo le fa'ailoaina o le PCR o le EDTA anticoagulated toto atoa.(E aofia ai: toto o le tagata, toto isumu, toto moa, toto manu, toto povi, toto maile, ma isi)
Fa'asologa o galuega
Teuina ma le ola fa'asao
O lenei pusa e tatau ona teuina ile -20°C.
2× SuperEasyTMFaafefiloi-EDTA, pe a faʻaaoga soo, e mafai foi ona teuina i le 4 ℃ mo le teuina mo taimi pupuu (faʻaaoga i totonu ole 10 aso).
6 × DNA Loading Buffer, e mafai ona teuina i le 4 ° C poʻo le -20 ° C mo se taimi umi.