La'au laau Direct PCR Plus kit (e aunoa ma ni Sampling Tools) Protocol Direct PCR from Plant Material
Fa'amatalaga
O lenei oloa o lo'o fa'aogaina se faiga fa'apipi'i lysis e fa'amama ai laulaau.O le lysate e mafai ona faʻaaogaina e fai ma faʻataʻitaʻiga e aunoa ma le faʻamamaina.A maeʻa ona faʻaopoopo mea muamua, o le PCR Mix o lenei pusa e mafai ona faʻaogaina mo le faʻalauteleina.
Fa'amatalaga
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Vaega o pusa
Vaega I | Puipui PS1 |
Puipui P2 | |
6×DNA Loading Buffer | |
Vaega II | Puipui PS2 |
2× Laulau PCR FaigofieTMFaafefiloi |
Fa'apitoa & itu lelei
■ Leai se taimi e alu ai ma taugata DNA fa'amamāina
■ Laiti meafaitino
■ Faigofie-o le faʻataʻitaʻiga e mafai ona tuʻuina atu i le PCR reaction poʻo le lysis reaction e aunoa ma le tipiina pe olo
■ 2 × Faafefiloi, faʻaitiitia faʻataʻitaʻiga faʻapipiʻiina mea sese ma tali faiga taimi sauniuni
■ E mafai ona fa'amae'a le fa'ata'ita'iga fa'anatinati i le 10 minute, e mafai ona fa'amae'a le tali PCR i totonu ole 50 minute.
■ E mafai ona fa'amae'a le tali atu i le fa'aogaina-lysis maualuga ile 96-well PCR plate
Fa'asologa o pusa
Fa'aoga: Fa'ailoaina o la'au transgenic, genotyping, etc.
Fa'ata'ita'iga: lau la'au e mauoa i le polysaccharide po'o le polyphenol
Fa'ata'ita'iga tele: Diameter 2-3mm (auala tuusao), diameter 5-7mm (lysis method)
Su'esu'ega: PCR oloa ≤1kb