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itulau_fanua

La'au laau Direct PCR Plus kit (e aunoa ma ni Sampling Tools) Protocol Direct PCR from Plant Material

Fa'amatalaga o pusa:

Fa'aaoga sa'o le polysaccharide ma le polyphenol leaf lysate e fai ma fa'ata'ita'iga mo le fa'alauteleina o le PCR, e aunoa ma le fa'aesea o le DNA, vave ma maualuga le lagona.

Leai se taimi faʻaalu ma taugata faʻamamaina DNA (Taimi, galuega ma tau faʻasaoina!)

itiiti meafaitino

Faigofie-o le faʻataʻitaʻiga e mafai ona tuʻuina atu i le PCR reaction poʻo le lysis reaction e aunoa ma le tipiina pe olo

2 X Faafefiloi—faaitiitia o mea sese o le utaina o faataitaiga ma le taimi o sauniuniga faiga faiga

Anapogi—o le tapenaga o ata e mafai ona mae'a ile 10 minute, ole PCR e mafai ona mae'a ile 50 minute

E mafai ona fa'amae'aina le fa'aogaina o le lysis i le 96-well PCR plate

malosi mua'i

 

 

 


Fa'amatalaga Oloa

Faailoga o oloa

Fa'amatalaga

O lenei oloa o lo'o fa'aogaina se faiga fa'apipi'i lysis e fa'amama ai laulaau.O le lysate e mafai ona faʻaaogaina e fai ma faʻataʻitaʻiga e aunoa ma le faʻamamaina.A maeʻa ona faʻaopoopo mea muamua, o le PCR Mix o lenei pusa e mafai ona faʻaogaina mo le faʻalauteleina.

Fa'amatalaga

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Vaega o pusa

Vaega I Puipui PS1
Puipui P2
6×DNA Loading Buffer
Vaega II Puipui PS2
2× Laulau PCR FaigofieTMFaafefiloi

Fa'apitoa & itu lelei

■ Leai se taimi e alu ai ma taugata DNA fa'amamāina
■ Laiti meafaitino
■ Faigofie-o le faʻataʻitaʻiga e mafai ona tuʻuina atu i le PCR reaction poʻo le lysis reaction e aunoa ma le tipiina pe olo
■ 2 × Faafefiloi, faʻaitiitia faʻataʻitaʻiga faʻapipiʻiina mea sese ma tali faiga taimi sauniuni
■ E mafai ona fa'amae'a le fa'ata'ita'iga fa'anatinati i le 10 minute, e mafai ona fa'amae'a le tali PCR i totonu ole 50 minute.
■ E mafai ona fa'amae'a le tali atu i le fa'aogaina-lysis maualuga ile 96-well PCR plate

Fa'asologa o pusa

Fa'aoga: Fa'ailoaina o la'au transgenic, genotyping, etc.

Fa'ata'ita'iga: lau la'au e mauoa i le polysaccharide po'o le polyphenol

Fa'ata'ita'iga tele: Diameter 2-3mm (auala tuusao), diameter 5-7mm (lysis method)

Su'esu'ega: PCR oloa ≤1kb

Fa'agasologa o galuega

la'au la'au sa'o PCR fa'aopoopo-faigaluega

Electropherogram

lau la'au sa'o PCR fa'aopoopo

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