Fatu La'au (Polysaccharide Polyphenol tama'i) Tu'usa'o PCR Plus Kit I (e aunoa ma Meafaigaluega Fa'ata'ita'iga)
Fa'amatalaga
O lenei oloa o loʻo faʻaaogaina se faiga faʻapitoa lysis reaction system, lea e mafai ona vave tuʻuina atu genomic DNA mai faʻataʻitaʻiga fatu laau ma polysaccharides maualuga ma polyphenols (e pei o faʻi, cotton, ma isi) mo le faʻaogaina i PCR reactions, o lea e fetaui lelei mo suʻesuʻega faʻatupu tele.Ina ia fa'amalieina mana'oga o su'ega su'esu'ega 'ese'ese, e mafai e lenei pusa ona fa'aogaina le tele o ituaiga fa'ata'ita'iga (e pei o fatu atoa, tipi'i laiti, po'o fa'ata'ita'iga eleele o fatu e tele) mo su'ega.Faatasi ai ma i latou, mo faʻataʻitaʻiga fatu o loʻo manaʻomia pea ona faʻatupuina, e mafai ona e filifili e tipi ese ni fasipisi micro-tissue (1-5mg) e ese mai i fatu embryos mo suʻega;mo faʻataʻitaʻiga e manaʻomia ai faʻataʻitaʻiga ma suʻega i se numera tele o faʻataʻitaʻiga, e mafai ona e filifili e faʻafefiloi le tele o faʻataʻitaʻiga ma tuʻufaʻatasia.A maeʻa ona oloina i ni vaega e tusa ma le 0.5mm le lautele, e mafai ona faia le faʻataʻitaʻiga (a itiiti ifo i le 0.1% o le faʻataʻitaʻiga e mafai ona iloa).
Fa'amatalaga
50T,200T,500T,2000T
Vaega o pusa
Fua Toto Direct PCR Plus Kit I Polysaccharide Polyphenol Fuga La'au (Laiti) Pusa PCR Direct | |||||
Fausia pusa | TP-0315T | TP-03151 | TP-03152 | TP-03153 | |
50 T | 200 T | 500 T | 2000 T | ||
Vaega I | Puipui SSP1 | 4.5ml | 18ml | 45ml | 90ml×2 |
Puipui SP2 | 3ml | 10ml | 25ml | 100ml | |
6x DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
Vaega II | Puipui SSP2 | 1.5ml | 6ml | 15ml | 60ml |
2× Seed PCR FaigofieTMFaafefiloi | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 | |
Tusi Lesona | 1 | 1 | 1 | 1 |
Fa'ailoga ma mea lelei
- E leai se taimi e alu ai ma taugata DNA e manaʻomia.
- E laʻititi le manaʻoga faʻataʻitaʻi, naʻo le ave o le fatu e tasi.
- E leai ni togafitiga faʻapitoa e pei o le oloina ma le tuʻimomomoina e manaʻomia, ma e faigofie le taʻaloga.
- O le faiga o le PCR sili ona lelei e mafai ai e le PCR ona maua le maualuga maualuga ma le malosi o le faapalepale i le PCR reaction inhibitors.
Fa'asologa o galuega
Tulaga teuina
1. Felauaiga
O le faagasologa atoa o felauaiga pusa aisa maualalo-vevela, ina ia mautinoa o le pusa Vaega II o loʻo i se setete o <4 ℃.
2. Teuga
Vaega I o lenei pusa e tatau ona teuina i le vevela o le potu poʻo le 2-8°C.
Vaega II e tatau ona teuina ile -20°C.