Lnc-RT Heroᵀᴹ I(Faatasi ai ma le gDNase)(Super Premix mo le fa'asologa muamua o le cDNA mai le lncRNA)
Fa'amatalaga
25×20μl Rxns, 50×20μl Rxns
Lnc-RT HeroTM I (Fa'atasi ai ma le gDNase) o se faiga fa'aliliuga fa'aliliu fa'apitoa na atia'e mo lncRNA e vave aveese ai le fa'aleagaina o le DNA genomic.5×gDNase Mix e mafai ona vave aveese le genome o totoe i le RNA i le 42 ° C mo minute 2, ma aloese mai le faʻalavelaveina o le genome i luga o taunuuga qPCR.
5×L-RT HeroTM Mix o lo'o iai Foregene LncRNA Reverse Transcriptase na fa'atupuina fa'apitoa e Foregene, o se ituaiga fou o fa'aliliuga fa'aliliu fa'apitoa mo lncRNA ma isi fa'ata'ita'iga lavelave RNA umi, fa'atasi ai ma le malosi o le feso'ota'iga RNA ma le maualuga o le fa'amauina o le fa'aliliuina.O le faiga sili ona lelei e fa'avave ai le fa'asologa o fa'aliliuga fa'aliliuga ma faigofie ona fa'aliliuina fa'ata'ita'iga RNA fa'atasi ai ma le maualuga o le GC ma le lavelave o le fausaga lona lua.O le vaega muamua o le cDNA fa'apipi'i e mafai ona fa'amae'a ile 42°C ile 15 minute.
Vaega o pusa
| 5× gDNase Mix |
| 5× L-RT HeroTM Mix |
| RNase-Free ddH2O |
| Faatonuga |
Fa'apitoa & itu lelei
■Mafai lelei e aveese le gDNA, lea e mafai ona aveese le gDNA i le mamanu i totonu ole 2 minute.
■ E mafai ona fa'aliliu lelei le fa'aliliuga o le lncRNA.Pe a o'o i le qPCR le fa'aliliuga o oloa, o le tau o le Ct e la'ititi ifo nai lo le faiga fa'aliliuga masani.
■ Faiga fa'aliliuga fa'aliliuga lelei, e na'o le 15 minute e fa'amae'a ai le tu'ufa'atasiga o le strand cDNA muamua.
■ Fa'ata'ita'iga lavelave: fa'ata'ita'iga e iai le GC maualuga ma le lavelave o le fausaga lona lua e mafai fo'i ona toe fa'afo'i i se tulaga maualuga.
■ Faiga fa'aliliuga fa'aliliu maualuga, fa'ata'ita'iga pg-level e mafai fo'i ona maua le cDNA maualuga.
■ O le fa'aliliuga fa'aliliuina faiga e maualuga le mautu vevela, o le vevela tali sili ona lelei e 42℃, ma o loʻo i ai pea le lelei o faʻaliliuga faʻaliliuga i le 50℃.
Fa'aoga pusa
lncRNA su'esu'ega quantitative.
lncRNA su'esu'ega fa'atusa atoatoa.
E mafai ona vave ma sa'o le su'esu'eina o le RNA e pei ole RNA virus.
Fa'aliliuina fa'aliliuga o fa'ata'ita'iga RNA fa'atasi ai ma mea e maua i le GC maualuga po'o le fa'alavelave lavelave lona fausaga lua.
Fa'agasologa o galuega
Teuina ma le ola fa'asao
E tatau ona teuina le pusa ile -20°C. Teu le oloa ile pusaaisa vevela tumau ile -20°C i le taimi lava na maua ai.Afai e talafeagai tulaga e teu ai, o le a le faʻaleagaina e le oloa soʻo se faʻatinoga i totonu ole 1-tausaga le vaitaimi aoga.
Taiala Iloiloga o Faafitauli
The following is an analysis of the problems that may be encountered in the use of Lnc-RT HeroTM I (With gDNase) series kits, hoping to be helpful to your experiments. In addition, we have dedicated technical support to help you with other experimental or technical problems beyond the operating instructions and questions. If you have any needs, please contact us: 028-83361257 or E-mail: Tech@foregene.com.
Non-fa'ateleina fa'apitoa
1. Fa'ailoga muamua le talafeagai
Fautuaga: Fuafua mea muamua e tusa ai ma mataupu faavae mamanu muamua.
2. Genome toega.
Fautuaga: Ia mautinoa o le laasaga muamua o le gDNA aveese le vevela o le 42 ° C, ma o le taimi tali e mafai foi ona faʻalautele i le 5min.
Leai se faʻailoga faʻateleina e le RT-qPCR
1. Ua fa'aleagaina le RNA.
Fautuaga: E tatau ona fou le mea e maua mai ai le RNA, ma e tatau ona faʻaogaina le RNA maualuga ma maualuga le mama.
2. O le RNA o lo'o i ai ni fa'alavelave.
Fautuaga: Reverse transcription inhibitors e masani ona aofia ai le SDS, guanidine salts, EDTA, ma isi. E fautuaina e fufulu le RNA precipitate i le 70% ethanol e aveese ai le inhibitors.
3. Fa'amatalaga autu o le mamanu.
Fautuaga: E tusa ai ma le faʻataʻitaʻiga faʻavae mamanu, toe faʻaleleia mea muamua mo le asiasiga.
O lo'o fa'aalia le fa'ailoga fa'atatau i le pule avanoa
1. Fa'aleagaina o meafaigaluega fa'aogaina po'o mea fa'afo'i.
Fautuaga: E tatau ona fa'a'ato'atoa uma mea fa'afofo po'o mea faigaluega mo le su'ega.Fa'aeteete ma agamalu pe a fetagofi ina ia puipuia fa'ata'ita'iga DNA mai le mitiia i totonu o le paipa pe masa'a i fafo mai le faagaau fa'aluma.
2. Fa'aleagaina na tupu i le taimi o le sauniuniga o le PCR reaction system.
Fautuaga: Fa'asagatau tatau pe a fetagofi, e pei o le: ofuina o totigilima latex, fa'aogaina o fa'amatalaga faamama.Fa'aoga le Fa'afefiloi PCR Taimi Moni i se faiga fa'ama'i fa'ama'i.
3. E fa'aleaga mea muamua.
Fautuaga: Fa'aaoga le SDS-PAGE electrophoresis e su'esu'e ai pe fa'aleaga mea muamua, ma sui i fa'amatalaga fou mo su'ega su'esu'ega o le fluorescence.
Tusi Taiala:
