Foregene DNA Identification System 30A (E le'o Aveesea)
Fa'amatalaga o pusa:
FOREGENE DNA identification system 30A fa'aoga ono-lanu fa'aigoa fa'ailoga efa'atele 29autosomalSTR nofoaga, tasiAmel locus ma le tasi Yindel locus i le taimi e tasi, ma e mafai ona faʻalauteleina saʻo pepa faamama, FTAkata, fufui fuifui, pepa faua,ma le mfafo o le swab.
Fa'amatalaga
FOREGENE DNA identification system 30A fa'aoga ono-lanu fa'aigoa fa'ailoga efa'atele 29autosomalSTR nofoaga, tasiAmel locus ma le tasi Yindel locus i le taimi e tasi, ma e mafai ona faʻalauteleina saʻo pepa faamama, FTAkata, fufui fuifui, pepa faua,ma le mfafo o le swab.
Pusa mea
Sauniuniga Faiga Fa'aopoopo
Fuafuaga 1: Le saunia o le tuufaatasiga o le Faʻasalalauga Faʻasalalau (E leai se totogi) faʻateleina
|
| Ctagata tetee | 25 μl faiga (μl) | 10 μl faiga (μl) |
| Master Mix | 4 × Master Mix VII | 6.25 | 2.5 |
| 5 x 30AFa'afefiloi Muamua | 5.0 | 2 | |
| Vai fa'aonaona | 13.75 | 5.5 | |
| Blod pisia | 1.2mm le lautele | 1.2mm le lautele | |
| Tle tele o tali | 25 μl | 10 μl | |
Fa'amatalaga Fa'amatalaga
Fa'aopoopo Kalafi 1:Allelic Ladder Types Map
Fa'aopoopo Kalafi 2:DNA ta'i fa'asologa 9948 ta'ifaafanua
Reagent Teuga
1. Fa'amolemole teu i lalo -20°C,, pe a uma ona maua pusa fa'aaisa i le aisa mago po'o le pusa aisa gel.
2. Fa'amolemole teu le vaega muamua 4xpremix VII i le -20°C, pe a uma ona ave le pusa mo le fa'aaogaina, ma teu isi mea e totoe muamua i le 4°C e aloese ai mai le toe fa'amaisa ma fa'afefe.Afai e laʻititi le vailaʻau e tasi, e fautuaina e teu i le -20 ° C pe a uma ona faʻatau,
3. Taofi vaega pe a uma le tali i le 4 ° C, aloese mai le faʻamalo faʻasolosolo ma le faʻafefe, ma aua neʻi paʻi i mea faʻafefe aʻo leʻi faia le tali e aloese ai mai le faʻaleagaina.
