Foregene DNA Identification System 20A (Aso'ese fua ole DNA)
Fa'amatalaga o pusa:
Foregene DNA identification system 20A e fa'aogaina le fa'aigoa fa'ailoga e lima lanu e fa'ateleina ai le 19 STR loci ma le 1 sex loci i totonu o se paipa e tasi.A'o ufiufi le 13 CODIS core loci, 20 core loci e fetaui ma mana'oga a le Matagaluega o le Puipuiga a le Malo mo le autosomal STR kit.O ia o le lima taumatau o le suʻesuʻeina o DNA faʻamaumauga faʻamaumauga ma faʻamaoniga faʻafaamasinoga.
Fa'amatalaga
Foregene DNA identification system 20A e fa'aogaina le fa'aigoa fa'ailoga e lima lanu e fa'ateleina ai le 19 STR loci ma le 1 sex loci i totonu o se paipa e tasi.A'o ufiufi le 13 CODIS core loci, 20 core loci e fetaui ma mana'oga a le Matagaluega o le Puipuiga a le Malo mo le autosomal STR kit.O ia o le lima taumatau o le suʻesuʻeina o DNA faʻamaumauga faʻamaumauga ma faʻamaoniga faʻafaamasinoga.
Pusa mea
| Kit Componet | Igoa Vaega | Volume（μl/tupa） | Aofa'i(tupa) |
| PCR mua'i tali fa'afoliga | 2.5×PCR tali fa'afefete Ⅲ | 1000 | 2 |
| 5×20A fa'afefiloi muamua | 500 | 2 | |
| Puleaina DNA 9947A(1ng/μl) | 25 | 1 | |
| Vai fa'aonaona | 1700 | 2 | |
| Taq Polymerase | Taq polymerase Ⅲ | 40 | 2 |
| PCR Reaction reagent | Apefai Allelic20A | 40 | 1 |
| O le mamafa o le molecular standard i totonu ORG 500 | 150 | 2 | |
| Manatua | |||
Fa'amatalaga Fa'amatalaga
1. Tatala le polokalama Genemapper ID.A faʻaaogaina lenei pusa mo le taimi muamua, e te manaʻomia le faʻaulufaleina mai o paneta, seti talone, metotia suʻesuʻega tutusa, ma le lapoʻa masani (ORG500: 65, 70, 80, 100, 120, 140, 160, 180, 200, 225, 250, 275, 30, 30, 30, 30, 30, 30, 30, 30, 30, 30). 0, 490, 500)
2. Faʻaulufale mai faʻamaumauga o le electrophoresis, filifili faʻasologa o suʻesuʻega tutusa e pei o le laulau, metotia auʻiliʻili, ma le tele o le tulaga, ma suia le ituaiga faʻataʻitaʻiga o le Apefai i le "Allelic Ladder" i le "Sample Type" column;amata su'esu'e fa'amaumauga.
Reagent Teuga
1. A maeʻa ona maua le pusa aisa i le aisa mago poʻo le pusa aisa palasitika, afai e le faʻaaogaina mo sina taimi, faʻamolemole teu i lalo -20 ° C mo se taimi umi.
2. A maeʻa ona ave i fafo le pusa ma faʻaaoga, e tatau ona teuina le pusa i le 4 ° C aʻo leʻi faia le tali e aloese ai mai le toe faʻamalo ma faʻafefe;Taq enzyme e tatau ona teuina ile -20°C.
3. A maeʻa le tali, e tatau ona teuina le pusa (e aofia ai le mamafa o le molecular i totonu ma le apefaʻi) i totonu o le "electrophoresis detection room" i le 4 ° C, aloese mai le toe faʻamaisaina ma le faʻafefe, ma aloese mai le faʻafesoʻotaʻi ma le pusa muamua e faʻafefe ai le faʻaleagaina.
