Foregene DNA Identification System 27Y
Fa'amatalaga o pusa:
Foregene O le DNA Identification System 27Y e fa'aogaina ai tekinolosi fa'ailoga felanulanua'i ono-lanu e fa'alautele ai le 27 Y-STR loci i le taimi e tasi.E aofia ai: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS537S3, DYS583, DYS3F393 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
O le pusa o loʻo i ai le malosi e faʻasaʻo ai, ma e mafai ona faʻapipiʻi saʻo le pepa faamama, pepa FTA, cotton swab, saliva card, oral swab, ma e mafai foi ona faʻaaogaina e aveese ma faʻamamā ai mamanu DNA mai mea faʻapitoa.
Fa'amatalaga
Foregene O le DNA Identification System 27Y e fa'aogaina ai tekinolosi fa'ailoga felanulanua'i ono-lanu e fa'alautele ai le 27 Y-STR loci i le taimi e tasi.E aofia ai: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS537S3, DYS583, DYS3F393 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
O le pusa o loʻo i ai le malosi e faʻasaʻo ai, ma e mafai ona faʻapipiʻi saʻo le pepa faamama, pepa FTA, cotton swab, saliva card, oral swab, ma e mafai foi ona faʻaaogaina e aveese ma faʻamamā ai mamanu DNA mai mea faʻapitoa.
Pusa mea
| Pusa su'ega reagent | Igoa vaega | Volume (μl/faaga) | Aofa'i (tupa) |
| Pusa PCR muamua tali | 2×PCR Reaction Master Mix | 1250 | 2 |
| 5 x 27Y Fa'afefiloi Muamua | 500 | 2 | |
| Tulaga Tulaga DNA 9948 | 20 | 2 | |
| Vai fa'aonaona | 1700 | 2 | |
| Pusa Tali Fa'amuli-PCR | 27Y allele paluga Apefai | 20 | 2 |
| moli moli mole mamafa i totonu tulaga masani ORG500 | 150 | 2 |
Fa'amatalaga Fa'amatalaga
1. Tatala le polokalama Gene Mapper ID.A faʻaaogaina lenei pusa mo le taimi muamua, e te manaʻomia le faʻaulufaleina mai o paneli, seti talone, metotia suʻesuʻega tutusa, ma le lapoʻa masani (ORG500: 65, 70, 80, 100, 120, 140, 160, 180, 200, 225, 250, 275, 30, 30, 30, 30, 30, 30, 30, 30, 30, 30, 30, 25, , 490, 500).
2. Faʻaulufale mai faʻamaumauga o le electrophoresis, filifili faʻasologa o suʻesuʻega tutusa e pei o le laulau, metotia auʻiliʻili, ma le tele o le tulaga, ma suia le ituaiga faʻataʻitaʻiga o le Apefai i le "Allelic Ladder" i le "Sample Type" column;amata su'esu'e fa'amaumauga.
Reagent Teuga
1. Fa'amolemole teu i lalo -20°C (pe a le fa'aogaina mo sina taimi), pe a uma ona maua pusa fa'aaisa i totonu o le aisa mago po'o le gel aisa.
2. Fa'amolemole teu le pusa i le 4°C a'o le'i faia le tali e 'alofia ai le fa'a'aisa fa'asolo ma le fa'afefeteina, pe a uma ona ave i fafo le pusa mo le fa'aoga,;ole enzyme e tatau ona teuina ile -20°C.
2. A maeʻa le tali, e tatau ona teuina le pusa (e aofia ai le mamafa o le molecular i totonu ma le apefaʻi) i totonu o le "electrophoresis detection room" i le 4 ° C, aloese mai le toe faʻamaisaina ma le faʻafefe, ma aloese mai le faʻafesoʻotaʻi ma le pusa muamua e faʻafefe ai le faʻaleagaina.
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