EndoFree Maxi Plasmid Kit (Spin Column)
Fa'amatalaga o pusa:
O le faʻagasologa atoa o le aveeseina e naʻo le 1 itula, faʻamautinoa le faʻaogaina ma le vave.
Fa'amatalaga
O le EndoFree Maxi Plasmid Kit e faʻaaogaina se metotia faʻapipiʻi faʻapipiʻi silica membrane e faʻamaonia lelei ma faʻapitoa le DNA plasmid.I le tu'ufa'atasia o le Endotoxin Removal Buffer P4 ma EndoFree Maxi Filtration Columns, endotoxin, proteins ma isi mea leaga e mafai ona aveese lelei.O le faʻagasologa atoa o le aveeseina e naʻo le 1 itula, faʻamautinoa le faʻaogaina ma le vave.
Ole aofaʻi ole siama fautuaina mo faʻataʻitaʻiga taʻitasi: Mo le maualuga-kopi plasmid, e fautuaina e faʻaaoga le 100 ml o faʻasalalauga faʻaleaganuʻu siama, ma o le fua mai e masani lava ile 500-1500 μg.Mo plasmid maualalo-kopi, 200 ml siama faʻasalalauga faʻasalalauga fautuaina e faʻatupu ai le 200-600 μg plasmids.
Vaega o pusa
| Oloa Vaega | D909-E(10 tapenapenaga) |
| Puipui BL | 30 ml |
| Puipui P1 | 100 ml |
| Puipui P2 | 100 ml |
| Puipui P4 | 100 ml |
| Puipui DW2 | 44 ml |
| Puipui EB | 30 ml |
| RNase A(10 mg/ml) | 1 ml |
| DNAPure Maxi Spin Columns | 10 pcs |
| FinePure Maxi Filter Syringe | 10 pcs |
| 50 ml Tubes Aoina | 10 pcs |
Su'esu'ega ole Plasmid DNA Concentration ma le Mama
O le mama ma le faʻaogaina o le DNA plasmid na maua mai e mafai ona iloa e le electrophoresis gel agarose ma se spectrophotometer ultraviolet.Ole tau ole OD260 ole 1 e fetaui ile 50 μg/ml ole DNA fa'alua.Ole tau ole OD260/OD280 ole DNA plasmid fa'amamaina e masani ile va ole 1.7-1.9.O le DNA plasmid faʻamamaina e mafai ona faʻaoga saʻo i faʻataʻitaʻiga ma manaʻoga mama maualuga, e pei o le feaveaʻi o le cell poʻo faʻataʻitaʻiga i vivo.
Fa'aoga pusa
O le DNA plasmid e maua mai e lenei pusa e mafai ona faʻaaogaina i gaioiga masani masani, e aofia ai le faʻamaʻiina o le enzymatic, PCR, faʻasologa, ligation, suiga faʻapea foʻi ma le fesuiaiga mo ituaiga sela eseese.
Teuina ma le ola fa'asao
EndoFree Maxi Plasmid Kit e mafai ona teuina i le vevela o le potu (15-25°C) i lalo ole tulaga mago mo le 12 masina.Fa'aopoopo le RNase A ile Buffer P1 a'o le'i fa'aoga muamua, fa'afefiloi lelei ma teu ile 2-8°C.E mafai ona teuina lelei le paluga mo le sili atu i le 6 masina.
