Fatu La'au (Lapopoa) Pusa PCR sa'o II-UNG(e leai ni Meafaigaluega Fa'ata'ita'i)
Fa'amatalaga
O lenei oloa e faʻaaogaina se faiga faʻapitoa lysis reaction, lea e mafai ona vave faʻasaʻo le DNA genomic mai faʻataʻitaʻiga fatu laau ma le maualalo o le polysaccharide ma le polyphenol (e pei o: araisa, saito, tapaa, sana, soya, ma isi) mo le faʻaaogaina i PCR reactions, o lea e sili ona talafeagai Large-scale genetic testing.Ina ia fa'amalieina mana'oga o su'ega su'esu'ega 'ese'ese, e mafai e lenei pusa ona fa'aogaina le tele o ituaiga fa'ata'ita'iga (e pei o fatu atoa, tipi'i laiti, po'o fa'ata'ita'iga eleele o fatu e tele) mo su'ega.Faatasi ai ma i latou, mo faʻataʻitaʻiga fatu o loʻo manaʻomia pea ona faʻatupuina, e mafai ona e filifili e tipi ese tipi tipi (1-5mg) e ese mai i le fatu fatu mo le faʻataʻitaʻiga;mo faʻataʻitaʻiga e manaʻomia ona faʻataʻitaʻiina i le tele o faʻataʻitaʻiga, e mafai ona e filifili e faʻafefiloi le tele o faʻataʻitaʻiga ma tuʻufaʻatasia.A maeʻa ona oloina i ni vaega e tusa ma le 0.5mm le lautele, e mafai ona faia le faʻataʻitaʻiga (a itiiti ifo i le 0.1% o le faʻataʻitaʻiga e mafai ona iloa).
Fa'amatalaga
50T,200T,500T,2000T
Vaega o pusa
Fua Fa'ato'a Tu'usa'o PCR Kit II-UNG Fatu La'au (Lapopoa) Direct PCR Kit-UNG | |||||
Fa'atulagaina o pusa | TP-0314T | TP-03141 | TP-03142 | TP-03143 | |
50 T | 200T | 500T | 2000T | ||
Vaega I | Puipui SP1 | 30ml | 120ml | 100ml×3 | 400ml×3 |
Puipui SP2 | 3ml | 10ml | 25ml | 100ml | |
6x DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
Vaega II | 2× Seed PCR FaigofieTMFa'afefiloi(UNG) | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
Tusi Lesona | 1 | 1 | 1 | 1 |
Fa'ailoga ma mea lelei
- E le mana'omia le fa'amamāina ole DNA ole taimi ma taugata.
- E laʻititi le manaʻoga faʻataʻitaʻi, naʻo le ave o le fatu e tasi.
- E leai ni togafitiga faʻapitoa e pei o le oloina ma le tuʻimomomoina e manaʻomia, ma e faigofie le taʻaloga.
- O le faiga o le PCR sili ona lelei e mafai ai e le PCR ona maua le maualuga maualuga ma le malosi o le faapalepale i le PCR reaction inhibitors.
- Polokalama PCR Anti-filogia 2× Seed PCR FaigofieTMFaʻafefiloi (UNG) e mafai ona faʻaumatia lelei le faʻaleagaina e mafua mai i oloa PCR ma faʻamautinoa le faʻapitoa ma le saʻo o le faʻalauteleina.
Fa'asologa o galuega
Tulaga teuina
1. Felauaiga
O le faagasologa atoa o felauaiga pusa aisa maualalo-vevela, ina ia mautinoa o le pusa Vaega II o loʻo i se setete o <4 ℃.
2. Teuga
Vaega I o lenei pusa e tatau ona teuina i le vevela o le potu poʻo le 2-8°C.
Vaega II e tatau ona teuina ile -20°C.