PCR Heroᵀᴹ
Fa'amatalaga o pusa:
◮Fa'amaoni maualuga: 6taimi o le enzyme masani Taq;
◮Saosaoa asaoasaoa fa'aupuga:o le saoasaoa faʻateleina o le 5-10sec / kb, lea e 6-12 taimi o le enzyme masani Taq;
◮Sili atu fa'ata'ita'iga fetuutuunai: e mafai ona fa'atupuina lelei fa'ata'ita'iga DNA lavelave fa'atasi ma mea e maua i le GC maualuga ma faigata ona fa'alautele;
◮MaualugaFa'atelega lelei: o le numera o taamilosaga faʻateleina e maualalo ifo nai lo le masani Taq enzyme;
◮Stronger Fa'apalepale si'osi'omaga: tu'u ile 37°C mo le vaiaso, fa'atumauina sili atu90%gaoioiga;
◮E iai le 5'→3' DNA polymerase activity ma le 5'→3' exonuclease activity, e aunoa ma le 3'→5' exonuclease activity.
Fa'amatalaga Pusa
O lenei oloa e aofia ai le tupulaga fou tulaga ese a Foregene o Taq——Foregene Taq Hero, optimized dNTPs, MgCl2, tali fa'apolopolo, PCR fa'aleleia atili, fa'asili ma fa'amautu.Le 2 × PCR HeroTMFa'afefiloi faiga e maualuga atu le fa'apalepale i PCR iniseti nai lo le PCR Mix faiga masani, ma e mafai ona faigofie ona taulimaina PCR amplification o mamanu lavelave eseese.O le faiga faʻapitoa faʻaalia ma le maualuga o le Taq Hero e faʻaalia ai le tali a le PCR e sili atu le faʻaleleia atili, faʻapitoa ma le maaleale.
Fa'amatalaga
Fa'asologa o Kit
PH-01013:2× PCR HeroTMFaafefiloi 1.7ml ×3
PH-01014:2× PCR HeroTMFaafefiloi 1.7ml ×6
PH-01015:2× PCR HeroTMFa'afefiloi 1.7ml ×24
Vaega o pusa
2× PCR HeroTMFaafefiloi e aofia ai: 0.1U Taq Hero/μl, 400μM dNTPs, 20mM Tris-HCl(pH8.8),100mM KCl, 5mM MgCl2,isi stabilizers ma enhancers.
Fa'apitoa & itu lelei
-Fa'amaoni maualuga:6taimi o le enzyme masani Taq;
- Saosaoa asaoasaoa fa'aupuga:o le saoasaoa faʻateleina o le 5-10sec / kb, lea e 6-12 taimi o le enzyme masani Taq;
-Sili atu fa'ata'ita'iga fetuutuunai: e mafai ona fa'atupuina lelei fa'ata'ita'iga DNA lavelave fa'atasi ma mea e maua i le GC maualuga ma faigata ona fa'alautele;
- MaualugaFa'atelega lelei: o le numera o taamilosaga faʻateleina e maualalo ifo nai lo le masani Taq enzyme;
-Stronger Fa'apalepale si'osi'omaga: tu'u ile 37°C mo le vaiaso, fa'atumauina sili atu90%gaoioiga;
-E iai le 5'→3' DNA polymerase activity ma le 5'→3'exonuclease activity, e aunoa ma le 3'→5'exonuclease activity.
Fa'aoga pusa
- PCR faʻalauteleina o vaega DNA
- Fa'ailoga DNA
- DNA fa'asologa
- PCR fa'atasi ma le si'usi'u
Teuina ma le ola fa'asa
E mafai ona teuina mo se taimi umi ile -20°C;mo le faʻaaogaina masani, e mafai ona teuina i le 4 ° C mo sina taimi puupuu.
