Tulaga Maualuga Saina Tau sili ona lelei Bioer Bsa21-Bsa30 Bio Dl100/200/2000/Bio PCR Q/Bio S50/Bio DNA Fa'ailoga Gaosiga Gaosia 2021
Fa'amatalaga o pusa:
O le 100bp DNA Ladder o loʻo aofia ai le 13 faʻaluaina o le DNA, lea e faʻaluaina le faʻaogaina o le 200bp, 500bp, ma le 1000bp, lea e fesoasoani e foia ai le faʻafitauli o vaega pupuu e vaivai i le susulu ma faigata ona iloa le va o fusi.I luga aʻe o le 1000bp, e tolu fusi o le 1200bp, 1500bp, ma le 2000bp ua faʻaopoopoina, lea e mafai ona faʻatulagaina e tusa ai ma le tele o le vaega DNA i le va o le 1000-2000bp.
E masani ona tatou tumau i le mataupu faavae "Uiga sili e muamua, mamalu maualuga".Ua matou tuuto atoatoa i le tuʻuina atu ia matou tagata faʻatau oloa faʻatauvaʻa maualuga, tuʻuina atu vave ma tuʻuina atu tomai mo le Top Quality China Best Price Bioer Bsa21-Bsa30 Bio Dl100/200/2000/Bio PCR Q/Bio S50/Bio DNAFaailogaManufacturer Production 2021, Ia mautinoa e te lagona le matua leai o se totogi e talanoa mai ai ia i matou mo le faʻatulagaina.ma matou manatu o le a matou fa'asoa atu le poto masani fa'atino fefa'ataua'iga ma a matou fa'atau oloa uma.
E masani ona tatou tumau i le mataupu faavae "Uiga sili e muamua, mamalu maualuga".Ua matou tuuto atoatoa i le tuʻuina atu ia matou tagata faʻatau oloa faʻatauvaʻa maualuga, tuʻuina atu vave ma tuʻuina atu tomai moSaina DNA Faailoga, Faailoga, I le taimi nei, faatasi ai ma le atinaʻeina o le initaneti, ma le tulaga o faʻasalalauga faavaomalo, ua matou filifili e faʻalautele pisinisi i maketi i fafo.Fa'atasi ai ma le fa'atonuga o le fa'asili atu o tupe mama i tagata fa'atau mai fafo e ala i le tu'u sa'o i fafo.O lea ua suia ai lo matou mafaufau, mai le fale i fafo, faʻamoemoe e tuʻuina atu ia matou tagata faʻatau tupe sili atu, ma tulimatai atu i le tele o avanoa e fai ai pisinisi.
Fa'amatalaga
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Vaega o pusa
| Vaega I | Puipui P1 |
| Puipui P2 | |
| 6 × DNA Loading Buffer | |
| Vaega II | 2× Laulau PCR FaigofieTMFa'afefiloi(UNG) |
Fa'apitoa & itu lelei
■ Leai se taimi e alu ai ma taugata DNA fa'amamāina
■ Laiti meafaitino
■ Faigofie-o le faʻataʻitaʻiga e mafai ona tuʻuina atu i le PCR reaction poʻo le lysis reaction e aunoa ma le tipiina pe olo
■ 2 × Faafefiloi, faʻaitiitia faʻataʻitaʻiga faʻapipiʻiina mea sese ma tali faiga taimi sauniuni
■ E mafai ona fa'amae'a le fa'ata'ita'iga fa'anatinati i le 10 minute, e mafai ona fa'amae'a le tali PCR i totonu ole 50 minute.
■ E mafai ona fa'amae'a le tali atu i le fa'aogaina-lysis maualuga ile 96-well PCR plate
Fa'asologa o pusa
Fa'aaogāina: fa'ailoga fa'avasegaina, fa'a-genotyping, ma isi.
Fa'ata'ita'iga: lau la'au
Fuafuaga: Diameter 2-3mm (auala tuusao), diameter 5-7mm (lysis method)
Va'aiga su'esu'e: vaega fa'atatau ≤1kb
Fa'agasologa o galuega
Electropherogram
E lua vaiaso ole su'esu'ega ole PCR Mix i lalo ole tulaga ole fa'aleagaina ole oloa fa'ateleina le PCR.E masani ona tatou tumau i le fa'avae "Uiga Sili muamua, Fa'ailoga Sili".Ua matou tuuto atoatoa i le tuʻuina atu ia matou tagata faʻatau oloa faʻatauvaʻa maualuga, tuʻuina atu vave ma tuʻuina atu tomai mo le Top Quality China Best Price Bioer Bsa21-Bsa30 Bio Dl100/200/2000/Bio PCR Q/Bio S50/Bio DNAFaailogaManufacturer Production 2021, Ia mautinoa e te lagona le matua leai o se totogi e talanoa mai ai ia i matou mo le faʻatulagaina.ma matou manatu o le a matou fa'asoa atu le poto masani fa'atino fefa'ataua'iga ma a matou fa'atau oloa uma.
Tulaga MaualugaSaina DNA Faailoga, Marker, O lea, faatasi ai ma le atinaʻeina o le initaneti, ma le tulaga o faʻasalalauga faʻavaomalo, ua matou filifili e faʻalautele pisinisi i maketi i fafo.Fa'atasi ai ma le fa'atonuga o le fa'asili atu o tupe mama i tagata fa'atau mai fafo e ala i le tu'u sa'o i fafo.O lea ua suia ai lo matou mafaufau, mai le fale i fafo, faʻamoemoe e tuʻuina atu ia matou tagata faʻatau tupe sili atu, ma tulimatai atu i le tele o avanoa e fai ai pisinisi.
