Saina Saina Si'osi'omaga Saina Taimi uma Taimi sili ona lelei tau-lelei PCR Gaosi ma Fa'atau |Foregene

Saina Si'osi'omaga Saina I Vaitau atoa Tulaga sili ona lelei tau-lelei PCR

Saina Si'osi'omaga Saina Taimi uma Taimi sili ona lelei tau-lelei PCR Ata Fa'aalia

Fa'amatalaga o pusa:

O lenei pusa o loʻo faʻaaogaina se faiga faʻapipiʻi lysis faʻapitoa e faʻasaʻo vave ai le DNA genomic mai le zebrafish ma isi aano o iʻa iʻa, siʻusiʻu poʻo fuamoa fuamoa faʻataʻitaʻiga mo tali PCR, o lea e fetaui lelei mo suʻega faʻatagata tetele.O le faagasologa o le tuʻuina atu o DNA genomic mai le lysis buffer e maeʻa i totonu ole 10-30 minute ile 65°C.Leai se isi faiga e pei ole polotini ma le RNA aveese e mana'omia, ma o le DNA fa'amatuu e mafai ona fa'aoga e fai ma fa'ata'ita'iga mo le tali atu o le PCR.

2×PCR Easy TM Mix o loʻo i ai le faʻapalepale malosi i le PCR reaction inhibitors, ma e mafai ona faʻaogaina le lysate o le faʻataʻitaʻiga e faʻataʻitaʻiina o se faʻataʻitaʻiga mo le faʻaleleia lelei ma faʻapitoa.O lenei reagent o loʻo i ai ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, tali faʻafefe, PCR optimizer ma stabilizer.Faʻaaogaina faʻatasi ma le lysis buffer e mafai ona vave ma faigofie ona iloa faʻataʻitaʻiga, ma ei ai uiga o le maualuga o le lagona, malosi faʻapitoa, ma le mautu lelei.

O le D-Taq DNA polymerase o se DNA polymerase na fa'apitoa ona atia'e e Foregene mo tali sa'o PCR.O le D-Taq DNA polymerase e malosi lona fa'apalepale i ituaiga PCR fa'alavelave fa'alavelave, ma e mafai ona fa'atupuina lelei le aofa'i o DNA i faiga fa'alavelave lavelave eseese, ma e mafai ona o'o atu i le 2Kb/min le saoasaoa fa'aopoopo, lea e fetaui lelei mo le tali atu o le PCR Direct.

Auina imeli ia matou

Fa'amatalaga Oloa

Faailoga o oloa

O le ki i lo tatou manuia o le "Oloa Lelei Lelei, Taua talafeagai ma Auaunaga Lelei" mo Saina Wholesale Saina atoa Season Best Quality Cost-Effective PCR, "Quality", "faamaoni" ma le "auaunaga" o la tatou mataupu faavae.O lo matou faamaoni ma tautinoga e tumau ma le faaaloalo i lau auaunaga.Talanoa mai ia i matou i le Aso Mo nisi fa'amatalaga, fa'afeso'ota'i mai nei.
O le ki i lo tatou manuia o le "Oloa Lelei Lelei, Taua talafeagai ma Auaunaga Lelei" moTulaga sili ona lelei PCR, Saina All Season PCR, O la matou 'au e iloa lelei manaoga o maketi i atunuu eseese, ma e mafai ona tuʻuina atu mea lelei talafeagai i tau sili ona lelei i maketi eseese.Ua uma ona faʻatūina e le matou kamupani se 'au agavaʻa, fatufatuaʻi ma faʻamoemoe e atiaʻe tagata faʻatau ma le multi-win principle.

Fa'amatalaga

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Vaega o pusa

Vaega I	Puipui FP
	Foregene Protease
	6x DNA Loading Buffer
Vaega II	2×PCR Faigofie^TMFaafefiloi
Faatonuga

Fa'apitoa & itu lelei

■ E le mana'omia le fa'amamāina o le DNA e alu ai le taimi ma taugata.

■ E la'ititi le mana'oga fa'ata'ita'i, e tusa ma le 1mg i'a i'a po'o le 10 fuamoa i'a e mafai ona su'eina.

■ E leai ni togafitiga faʻapitoa e pei o le oloina ma le tuʻimomomoina e manaʻomia, ma o le taotoga e faigofie.

■ Optimized PCR system e maua ai le PCR e maualuga atu le fa'apitoa ma le malosi o le fa'apalepale i PCR tali fa'alavelave.

Fa'asologa o pusa

Fa'aoga: I'a Sebra ma isi i'a vai fou.

DNA fa'amatu'u mai le fa'ata'ita'iga lysis: na'o le fa'aaogaina o se ata PCR.

O le pusa e mafai ona faʻaogaina mo faʻamoemoega nei: faʻamaonia o le transgenosis, faʻasologa o manu, ma isi.

Fa'agasologa o galuega

wk-flw-sebra-fish-direct-PCR

Teuina ma le ola fa'asao

Vaega I o lenei pusa e tatau ona teuina ile 2-8℃.

E mafai ona teuina le Reagent Buffer FP mo le 12 masina i lalo o tulaga mago;e mafai ona teuina i le 2-8 ℃ mo le teuina umi.

v Reagent Foregene Protease ei ai se faiga tulaga ese.Ina ia mautinoa lona gaioiga ma le mautu, faʻamolemole teu i le 4 ℃ mo le 12 masina.

vReagent 6×DNA Loading Buffer e mafai ona teuina i le 4 ℃ poʻo le -20 ℃ mo se taimi umi.

Vaega II o lenei pusa e tatau ona teuina ile -20℃.

vReagent 2×PCR Easy TM Mix, pe a faʻaaoga soo, e mafai foi ona teuina i le 4 ℃ mo le teuina mo taimi pupuu (faʻaaoga i totonu ole 10 aso).

O le ki i lo tatou manuia o le "Oloa Lelei Lelei, Taulaga talafeagai ma Auaunaga Lelei" mo Saina Wholesale China All Season Best Quality Cost-Effective PCR 185 / 70r13, "Quality", "faamaoni" ma le "auaunaga" o la tatou mataupu faavae.O lo matou faamaoni ma tautinoga e tumau ma le faaaloalo i lau auaunaga.Talanoa mai ia i matou i le Aso Mo nisi fa'amatalaga, fa'afeso'ota'i mai nei.
China Wholesale China All Season PCR, Best Quality PCR, E iloa lelei e le matou 'au manaoga maketi i atunuu eseese, ma e mafai ona tuʻuina atu mea lelei i tau sili ona lelei i maketi eseese.Ua uma ona faʻatūina e le matou kamupani se 'au agavaʻa, fatufatuaʻi ma faʻamoemoe e atiaʻe tagata faʻatau ma le multi-win principle.

Muamua: Falegaosimea Tu'u sa'o Saina Rna DNA Nucleic Acid Extraction Reagent

Sosoo ai: Fuafuaga Ta'uta'ua mo Saina Tu'u Fa'ata'ita'i Fa'ata'ita'iga Fa'alava ma Swabs mo Meafaigaluega Su'ega

Tusi lau savali iinei ma lafo mai ia i matou