SARS-CoV-2 Pusa Su'esu'ega Nucleic Acid (Metotia Su'esu'ega Fa'alili PCR Teleplex)
Fa'amatalaga o pusa:
◮ Fa'atonu PCR:Leai se mana'omia mo le nucleic acid extaction kit ma System, fa'amae'aina le 96 fa'ata'ita'iga ile 1 itula pe sili atu.
◮ Manaoga meafaigaluega maualalo ma sili ona fetuutuunai le faaaogaina:E manaʻomia naʻo le taimi moni PCR system .
◮ LoD ma maualuga lagona:E mafai ona o'o atu ile tulaga pito i lalo ole 500 kopi/ml le fa'alogoina ole su'esu'ega.
Fa'aaogāina Fa'amoemoe
Fa'avae ole su'ega
O lenei seti ma su'esu'ega ua fuafuaina mo le su'eina o fa'asologa fa'asao o le SARS-CoV-2 (ORF1ab gene, N gene ma E gene).O le pusa o lo'o i ai se Fa'atonuga Fa'alotoifale e fa'aoga e iloilo ai le lelei o fa'ata'ita'iga.O tekonolosi PCR taimi moni e fa'aogaina ai le polymerase chain reaction (PCR) mo le fa'alauteleina o fa'asologa o fa'asologa fa'apitoa ma su'esu'ega fa'apitoa mo le su'eina o le RNA fa'ateleina.O su'esu'ega o lo'o fa'aigoaina i le fa'aulutalatala fa'amalama ma le tineia vali.
Fa'amatalaga vave
◮Fa'amatalaga:48 Rxns/kit, 96 Rxns/kit
◮ Fa'apitoa:96.72%
Ma'ale'ale:97.92%
Vaega
| Leai. | Vaega | Aofaiga | Autu autu | |||
| 48 Rxns | 96 Rxns | |||||
| 1 | Fa'asa'olotoga o le nucleic acidsooupu | 1.5 mL/tulaga | 1 paipa | 1.5 mL/tulaga | 1 paipa | Surfactant |
| 2 | RNA puipuia | 27 μL/tulaga | 1 paipa | 53 μL/tulaga | 1 paipa | RNase inhibitor |
| 3 | SARS-CoV-2fofo tali | 800 μL / paipa | 1 paipa | 1600 μL/tulaga | 1 paipa | Primer, su'esu'e, tali fa'apolopolo, dNTP |
| 4 | SARS-CoV-2fa'afefiloi enzyme | 80 μL/tulaga | 1 paipa | 160 μL/tulaga | 1 paipa | Amata vevela Taq enzyme,M-MLV enzyme |
| 5 | SARS-CoV-2pulea lelei | 100 μL/tulaga | 1 paipa | 100 μL/tulaga | 1 paipa | Recombinant plasmid o lo'o iai le taula'igavaega, RNA |
| 6 | SARS-CoV-2pulea leaga | 1200 μL / paipa | 1 paipa | 1200 μL / paipa | 1 paipa | TE puipui |
Fa'apitoa & itu lelei
Fa'afefea ona fa'aoga
Metotia 1: Fa'atonu PCR auala
Metotia 2: Viral RNA Isolation + PCR
Metotia 1: Fa'aoga auala PCR tuusaoSui Fa'ata'ita'i Fa'ata'ita'i
Fa'asologa o le su'eina
Fe'avea'i ma Fa'aputuga
Faʻamaufaʻailogaina mai le malamalama ma teuina ile -20±5℃;
Ola fa'asa
1 tausaga
Fa'amatalaga Fa'atonu
