SARS-CoV-2 Pusa Su'esu'ega Nucleic Acid (Metotia Su'esu'ega Fa'alili PCR Teleplex)
Fa'amatalaga o pusa:
◮ Fa'atonu PCR:Leai se mana'omia mo le nucleic acid extaction kit ma System, fa'amae'aina le 96 fa'ata'ita'iga ile 1 itula pe sili atu.
◮ Manaoga meafaigaluega maualalo ma sili ona fetuutuunai le faaaogaina:E manaʻomia naʻo le taimi moni PCR system .
◮ LoD ma maualuga lagona:E mafai ona o'o atu ile tulaga pito i lalo ole 500 kopi/ml le fa'alogoina ole su'esu'ega.
Fa'aaogāina Fa'amoemoe
O le pusa lenei e fa'aogaina le tekinolosi RT PCR taimi moni (rRT-PCR) mo le su'esu'eina lelei o le SARS-CoV-2 nucleic acids i fa'ata'ita'iga swab o le nasopharyngeal po'o le oropharyngeal.
Fa'avae ole su'ega
O lenei seti ma su'esu'ega ua mamanuina mo le su'esu'eina o fa'asologa fa'asao o le SARS-CoV-2 (ORF1ab gene ma le N gene).O le pusa o lo'o i ai se Fa'atonuga Fa'alotoifale e fa'aoga e iloilo ai le lelei o fa'ata'ita'iga.
O tekonolosi PCR taimi moni e faʻaogaina ai le polymerase chain reaction (PCR) mo le faʻalauteleina o le faʻamoemoega patino
fa'asologa ma fa'atatau i su'esu'ega fa'apitoa mo le su'eina o le RNA fa'ateleina.Ua fa'ailogaina su'esu'ega
tusitala felanulanua'i ma vali tineia.
Fa'amatalaga vave
◮Fa'amatalaga:48 Rxns/kit, 96 Rxns/kit
◮ Fa'apitoa:96.72%
Ma'ale'ale:97.92%
◮Ituaiga fa'ata'ita'iga talafeagai
Nasopharyngeal Swab poo le Oropharyngeal Swab
◮Mea faigaluega talafeagai
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
Vaega
| Leai. | Vaega | Aofaiga | Vaega autu | |||
| 48 Rxns | 96 Rxns | |||||
| 1 | Nucleic acid fa'asa'olotoga | 1.4mL/fa'apa'u | 2 paipa | 5.3 mL/fagu | 1 fagu | Surfactant |
| 2 | RNA puipuia | 27 μL/tulaga | 1 paipa | 53 μL/tulaga | 1 paipa | RNase inhibitor |
| 3 | SARS-CoV-2 fofo tali | 800 μL / paipa | 1 paipa | 1600 μL/tulaga | 1 paipa | Primer, su'esu'e, tali fa'apolopolo, dNTP |
| 4 | SARS-CoV-2 fa'afefiloi enzyme | 80 μL/tulaga | 1 paipa | 160 μL/tulaga | 1 paipa | Amata vevela Taq enzyme, M-MLV enzyme |
| 5 | SARS-CoV-2 pulea lelei | 100 μL/tulaga | 1 paipa | 100 μL/tulaga | 1 paipa | Recombinant plasmid o loʻo i ai vaega faʻatatau, RNA |
| 6 | SARS-CoV-2 pulea le lelei | 1200 μL / paipa | 1 paipa | 1200 μL / paipa | 1 paipa | TE puipui |
Fa'apitoa & itu lelei
◮Fa'atonu PCR
Leai se mana'omia mo le nucleic acid extaction kit ma System, fa'amae'aina le 96 fa'ata'ita'iga ile 1 itula pe sili atu.
◮E maualalo mea e mana'omia ma fa'aogaina tele
E mana'omia na'o le taimi moni PCR system.
◮LoD ma maualuga lagona
E mafai ona o'o atu ile tulaga pito i lalo ole 500 kopi/ml le fa'alogoina ole su'esu'ega.
Fa'afefea ona fa'aoga
Metotia 1: Fa'atonu PCR auala
Metotia 2: Viral RNA Isolation + PCR
Metotia 1: Fa'aoga auala PCR tuusaoSui Fa'ata'ita'i Fa'ata'ita'i
Fa'asologa o le su'eina
Fe'avea'i ma Fa'aputuga
Faʻamaufaʻailogaina mai le malamalama ma teuina ile -20±5℃;
Ola fa'asa
1 tausaga
Fa'amatalaga Fa'atonu
Vitio Faagaoioi vave
Tusi lau savali iinei ma lafo mai ia i matou
