Matou te talitonu i: O mea fou o lo matou agaga ma le agaga.O le tulaga maualuga o lo tatou olaga.E mana'omia e le tagata fa'atau o lo tatou Atua mo Fa'ata'ita'iga fua fua mo Saina High Sensitivity DNA/Rna One-Step Probe Rt-QpcrKit V2, I totonu oa tatou fuafuaga, ua uma ona i ai le tele o faleoloa i Saina ma oa tatou fofo ua maua ai le viia mai tagata faatau i le lalolagi atoa.Faʻafeiloaʻi tagata faʻatau fou ma tuai e faʻafesoʻotaʻi i matou i le umi o le umi o mafutaga faʻapisinisi.
Matou te talitonu i: O mea fou o lo matou agaga ma le agaga.O le tulaga maualuga o lo tatou olaga.Tagata fa'atau mana'omia ona maua o lo tatou Atua moSaina Taq DNA Polymerase, Qpcr, Galulue malosi e faʻaauau pea le alualu i luma, faʻafouina i le alamanuia, faia taumafaiga uma i le atinaʻe muamua.Matou te taumafai i le mea sili matou te mafaia e fausia ai le faʻataʻitaʻiga faʻasaienisi, ia aʻoaʻoina le tele o tomai faʻapolofesa, ia atiaʻe le gaosiga o masini gaosiga ma le gaosiga o gaosiga, ia fatuina oloa muamua-valaau lelei, tau talafeagai, maualuga o le auaunaga, vave tuʻuina atu, e tuʻuina atu ia te oe le fatuina o tau fou.

Fa'amatalaga

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Vaega o pusa

Fa'apitoa & itu lelei

■ Leai se taimi e alu ai ma taugata DNA fa'amamāina
■ Laiti meafaitino
■ Faigofie-o le faʻataʻitaʻiga e mafai ona tuʻuina atu i le PCR reaction poʻo le lysis reaction e aunoa ma le tipiina pe olo
■ 2 × Faafefiloi, faʻaitiitia faʻataʻitaʻiga faʻapipiʻiina mea sese ma tali faiga taimi sauniuni
■ E mafai ona fa'amae'a le fa'ata'ita'iga fa'anatinati i le 10 minute, e mafai ona fa'amae'a le tali PCR i totonu ole 50 minute.
■ E mafai ona fa'amae'a le tali atu i le fa'aogaina-lysis maualuga ile 96-well PCR plate

Fa'asologa o pusa

Fa'aaogāina: fa'ailoga fa'avasegaina, fa'a-genotyping, ma isi.
Fa'ata'ita'iga: lau la'au
Fuafuaga: Diameter 2-3mm (auala tuusao), diameter 5-7mm (lysis method)
Va'aiga su'esu'e: vaega fa'atatau ≤1kb

Fa'agasologa o galuega

Electropherogram

E lua vaiaso ole su'esu'eina ole PCR Mix i'uga i lalo ole tulaga ole fa'aleagaina ole oloa fa'ateleina PCR.Matou te talitonu i le: Fa'afouina o lo tatou agaga ma le agaga.O le tulaga maualuga o lo tatou olaga.Tagata fa'atau mana'omia le maua o lo tatou Atua mo le Sa'olotoga fa'ata'ita'iga mo Saina High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2, I totonu oa tatou fuafuaga, ua uma ona i ai le tele o faleoloa i Saina ma oa tatou fofo ua maua ai le viia mai tagata faatau i le lalolagi atoa.Faʻafeiloaʻi tagata faʻatau fou ma tuai e faʻafesoʻotaʻi i matou i le umi o le umi o mafutaga faʻapisinisi.
Fa'ata'ita'iga fua moSaina Taq DNA Polymerase, Qpcr, Galulue malosi e faʻaauau pea le alualu i luma, faʻafouina i le alamanuia, faia taumafaiga uma i pisinisi muamua-vasega.Matou te taumafai i le mea sili matou te mafaia e fausia ai le faʻataʻitaʻiga faʻasaienisi, ia aʻoaʻoina le tele o tomai faʻapolofesa, ia atiaʻe le gaosiga o masini gaosiga ma le gaosiga o gaosiga, ia fatuina oloa muamua-valaau lelei, tau talafeagai, maualuga o le auaunaga, vave tuʻuina atu, e tuʻuina atu ia te oe le fatuina o tau fou.