Fa'atonu RT-qPCR Kit
Fa'amatalaga o pusa:
◮ Direct RT-qPCR Kit, faʻaaoga Foreasy Reverse Transcriptase ma Foreasy HS Taq DNA Polymerase atiaʻe e Foregene, faʻatasi ai ma faʻalavelave tali faʻapitoa, e faia ai lenei pusa ma malosi malosi ma fetaui, ma e mafai ona suʻeina saʻo le faʻaogaina o le Foregene Lysis system e fai ma faʻataʻitaʻiga.O lenei pusa e mafai ona faʻaoga mo le COVID-19 nucleic acid detection kit ma isi pathogenic bacteria nucleic acid detection kit development kit.
Fa'amatalaga
Direct RT-qPCR Kit e tu'u ese'ese le afifiina o le RT-qPCR pa'u ma le enzyme maualuga leleifaʻafefiloi (Taq, M-MLV, RI), faʻafaigofie mo le fatuina o pusa pito i tua ma faiga.fetuunaiga, i totonu o le faʻamaoniga faigofie.
Fa'amatalaga
500T, 50,000T
Vaega o pusa
| mea i totonu o pusa (20 μL faiga tali) | IM-05111-01 | IM-05112-01 |
| 500 T | 50,000 T | |
| Fa'afefiloi ole Enzyme Direct | 500 μL×1 | 50 ml×1 |
| 2× Direct RT-qPCR Pusa | 1 mL×5 | 500 ml×1 |
| Faatonuga | 1 | 1 |
Fa'agaioiga Laasaga
Aʻo leʻi saunia faʻamalosi, e tatau ona faʻafefe uma mea faʻapipiʻi i totonu o le pusa i le vevela o le potu ma faʻafefiloi malie.
A: Saunia of mamanu ma reagent
1. Saunia RNA template saunia (E fautuaina e faaaoga Foregene Total RNA Isolation Kit series kit e aveese ma faamama RNA template) poʻo le faʻataʻitaʻiga oloa taʻe (E fautuaina le faʻaogaina o le Foregene Lysis system), mea faʻapitoa (10 μM) ma isi mea faʻaaogaina talafeagai, meafaigaluega.
2. Tuu le 2× Direct RT-qPCR Buffer, RNase-Free ddH2O ma le 20× ROX Reference Dye (pe a fai's mana'omia) i totonu o le pusa ma le aisa, fa'afefeteina nei mea, ma fa'afefiloi malie.
B: Saunia of RT-qPCR faiga
Laupapa 1 : Saunia of RT-qPCR system
| Vaega | Volume | Fa'atonuga mulimuli |
| 2× Direct RT-qPCR Pusa | 10 μL | 1× |
| Fa'afefiloi ole Enzyme Direct | 1μL | |
| Fa'amuamua (10 μM) | 0.8 μL | 50-900nM |
| Fa'ata'ita'i Fa'asaga (10 μM) | 0.8 μL | 50-900nM |
| Su'ega(4 μM) | 1μL | 200nM |
| Fa'ata'ita'iga(RNA po'o le Lysate) | X μL | |
| 20×ROX Fa'ailoga Fa'ailoga | - | 1* |
| RNase-FreeddH2O | (6.4-X) μL | |
| Aofa'i Volume | 20μL |
C: Seti o le RT-qPCR tali polokalame
Laupapa 2 : RT-qPCR tali atuon polokalame faatulagaga (mo faataitaiga: lua-laa auala)
| Laa | vevela | Taimi | Ta'amilosaga | Anotusi | |
| 1 | 50℃ | 15 minu | 1* | 1 | Fa'aliliuga fa'aliliuga |
| 2 | 95℃ | 1 minu | 1 | mua'i denaturation | |
| 3 | 95℃ | 10 sec | 40-45 | Denaturation | |
| 60℃ | 30 sec 2* | Anneal/Fa'aopoopoga | |||
Teuina ma le ola fa'asao
-O le pusa e tatau ona teuina ile -20± 5℃.Ole vaitaimi ole aoga ole 12 masina.
- Aloese mai ta'amilosaga fa'a'aisa fa'aisa (<5 ta'amilosaga).
