Fa'aSaina Tomai Fa'apolofesa e sili ona lelei ma mautu le umi Taq DNA Polymerase fa'atasi ai ma le fa'apipi'iina o uta ma le dNTP PCR Kit.
Fa'amatalaga o pusa:
Fa'aaoga sa'o le polysaccharide polyphenol seed lysate e fai ma fa'ata'ita'iga mo le fa'alauteleina o le PCR, e aunoa ma le fa'aesea o le DNA, vave ma maualuga le lagona.O le UNG anti-pollution system ua faʻaopoopoina e puipuia ai mea sese.
- Leai se taimi faʻaalu ma taugata faʻamamaina DNA.
- E laʻititi le manaʻoga faʻataʻitaʻi, naʻo le ave o le fatu e tasi.
- E leai ni togafitiga faʻapitoa e pei o le oloina ma le tuʻimomomoina e manaʻomia, ma e faigofie le taʻaloga.
- O le faiga o le PCR sili ona lelei e mafai ai e le PCR ona maua le maualuga maualuga ma le malosi o le faapalepale i le PCR reaction inhibitors.
- Anti-pollution PCR system 2× Seed PCR EasyTM Mix (UNG), lea e mafai ona faʻaumatia lelei le faʻaleagaina e mafua mai i oloa PCR ma faʻamautinoa le faʻapitoa ma le saʻo o le faʻalauteleina.
O lo tatou tuputupu aʻe e faʻalagolago i oloa sili atu, taleni sili ma faʻamalosia malosi faʻatekonolosi mo Saina Tomai Faʻapolofesa Highly Efficient ma Stable Long Taq DNA Polymerase ma Loading Buffer ma dNTP PCR Kit, Faʻatasi ai ma le faʻaogaina o pulega faʻapisinisi, o le pisinisi e masani ona tuuto atu i le lagolagoina o faʻamoemoe e avea ma taʻitaʻi maketi o loʻo iai nei i a latou pisinisi.
O lo tatou tuputupu aʻe e faʻalagolago i oloa sili atu, taleni sili ma faʻamalosia malosi faʻatekonolosi moSaina PCR Amplification ma Qpcr, O loʻo galue la matou kamupani i le faʻatonuga o le "faʻavae faʻamaoni, galulue faʻatasi, faʻavae tagata, manumalo-manumalo galulue faatasi".Matou te faʻamoemoe e mafai ona matou maua se mafutaga faauo ma tagata fai pisinisi mai le lalolagi atoa.
Fa'amatalaga
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Vaega o pusa
| Vaega I | Puipui P1 |
| Puipui P2 | |
| 6 × DNA Loading Buffer | |
| Vaega II | 2× Laulau PCR FaigofieTMFa'afefiloi(UNG) |
Fa'apitoa & itu lelei
■ Leai se taimi e alu ai ma taugata DNA fa'amamāina
■ Laiti meafaitino
■ Faigofie-o le faʻataʻitaʻiga e mafai ona tuʻuina atu i le PCR reaction poʻo le lysis reaction e aunoa ma le tipiina pe olo
■ 2 × Faafefiloi, faʻaitiitia faʻataʻitaʻiga faʻapipiʻiina mea sese ma tali faiga taimi sauniuni
■ E mafai ona fa'amae'a le fa'ata'ita'iga fa'anatinati i le 10 minute, e mafai ona fa'amae'a le tali PCR i totonu ole 50 minute.
■ E mafai ona fa'amae'a le tali atu i le fa'aogaina-lysis maualuga ile 96-well PCR plate
Fa'asologa o pusa
Fa'aaogāina: fa'ailoga fa'avasegaina, fa'a-genotyping, ma isi.
Fa'ata'ita'iga: lau la'au
Fuafuaga: Diameter 2-3mm (auala tuusao), diameter 5-7mm (lysis method)
Va'aiga su'esu'e: vaega fa'atatau ≤1kb
Fa'agasologa o galuega
Electropherogram
E lua vaiaso o le PCR Mix detection results i lalo o le tulaga o le PCR amplification product contamination.Our tuputupu aʻe e faalagolago i oloa sili, taleni sili ma faʻamalosia malosi faʻatekonolosi mo Saina Professional Highly Efficient and Stable Long Taq DNA Polymerase with Loading Buffer and dNTP PCR Kit, Faatasi ai ma le lelei o le pulega o alamanuia, o le pisinisi e masani ona tuuto atu i le lagolagoina o maketi maketi o loʻo i ai nei.
Faapolofesa SainaSaina PCR Amplification ma Qpcr, O loʻo galue la matou kamupani i le faʻatonuga o le "faʻavae faʻamaoni, galulue faʻatasi, faʻavae tagata, manumalo-manumalo galulue faatasi".Matou te faʻamoemoe e mafai ona matou maua se mafutaga faauo ma tagata fai pisinisi mai le lalolagi atoa.
