Ua matou maua poto masani gaosi oloa.O le manumalo i le tele o au tusipasi taua o lana maketi mo le Super Purchasing mo Saina Real-Time PCR, Afai e te fiafia i toetoe lava o soʻo se oloa, ia manatua e matua lagona le saoloto e faʻafesoʻotaʻi ma matou mo nisi faʻamatalaga poʻo ia mautinoa e tuʻuina atu i matou imeli saʻo, matou te tali atu ia te oe i le na o le 24 itula faʻapea foʻi ma le upusii sili o le a tuʻuina atu.
Ua matou maua poto masani gaosi oloa.Manumalo le tele o au tusipasi taua o lana maketi moSaina PCR, Taimi Moni Pcr, Faʻatasi ai ma le agaga faʻamalosi o le "maualuga maualuga, faʻaogagofie, faʻaogaina ma mea fou", ma e tusa ai ma ia taʻiala o le "lelei lelei ae sili atu le tau," ma le "faʻaletupe faʻavaomalo", o loʻo matou taumafai e galulue faʻatasi ma kamupani vaega taʻavale i le lalolagi atoa e faia se paaga manumalo-manumalo.

Fa'amatalaga

O lenei pusa o loʻo faʻaaogaina se faiga faʻapipiʻi lysis faʻapitoa e mafai ona vave faʻasaʻo le RNA mai faʻataʻitaʻiga faʻaleaganuʻu mo le RT-qPCR tali, ma faʻaumatia ai le taimi faʻaalu ma le galue RNA faʻamamaina.E mafai ona maua le mamanu RNA ile na'o le 7 minute.O le 5×Direct RT Mix ma le 2×Direct qPCR Mix-SYBR reagents e saunia e le pusa e mafai ona vave ma lelei ona maua i'uga o le PCR fa'atusatusa i le taimi moni.

5 × Direct RT Mix ma 2 × Direct qPCR Mix-SYBR o loʻo i ai le faʻapalepale malosi o le inhibitor, ma o le lysate o faʻataʻitaʻiga e mafai ona faʻaaogaina e fai ma faʻataʻitaʻiga mo RT-qPCR saʻo.O lenei pusa o loʻo i ai le RNA maualuga-affinity Foregene reverse transcriptase, ma Hot D-Taq DNA polymerase, dNTPs, MgCl₂, tali fa'afefete, PCR optimizer ma fa'amautu.

Fa'amatalaga

200×20μl Rxns, 1000×20μl Rxns

Vaega o pusa

Fa'apitoa & itu lelei

■ Faigofie ma aoga : faʻatasi ai ma le Cell Direct RT tekinolosi, RNA faʻataʻitaʻiga e mafai ona maua i totonu o le 7 minute.

■ O le faʻataʻitaʻiga manaʻoga e laʻititi, e pei o le 10 sela e mafai ona suʻeina.

■ Tulaga maualuga: e mafai ona vave iloa le RNA i sela o loʻo faʻatupuina ile 384, 96, 24, 12, 6-well plates.

■ DNA Eraser e mafai ona vave aveese genomes tatala, faʻaitiitia le aʻafiaga i faʻataʻitaʻiga mulimuli ane.

■ Optimized RT ma qPCR system e sili atu ona lelei le fa'aliliuina o le RT-PCR la'asaga e lua ma sili atu ona patino le PCR, ma sili atu ona fa'asa'o ile fa'ainitaneti tali ole RT-qPCR.

Fa'aoga pusa

Avanoa o le faʻaogaina: sela faʻaleaganuʻu.

- RNA fa'amatuu mai ile fa'ata'ita'iga lysis: na'o le fa'ata'ita'iga RT-qPCR o lenei pusa.

- O le pusa e mafai ona faʻaoga mo faʻamoemoega nei: suʻesuʻega faʻamatalaga o kenera, faʻamaoniga o le siRNA-mediated gene silencing effect, suʻesuʻeina o fualaau, ma isi.

Ata

Teuina ma le ola fa'asao

Vaega I o lenei pusa e tatau ona teuina i le 4 ℃;Vaega II e tatau ona teuina ile -20℃.

Foregene Protease Plus II e tatau ona teuina i le 4℃, aua le fa'aisa ile -20℃.

Reagent 2×Direct qPCR Mix-SYBR e tatau ona teuina i le -20 ℃ i le pogisa;pe a faʻaaoga soo, e mafai foi ona teuina i le 4 ℃ mo le teuina mo taimi pupuu (faʻaaogaina i totonu ole 10 aso).O le manumalo i le tele o au faʻamaoniga taua o lana maketi mo le Super Purchasing mo Saina Q160/Q320 Real-Time PCR, Afai e te fiafia i toetoe lava o soʻo se oloa, ia manatua e matua lagona le saoloto e faʻafesoʻotaʻi ma matou mo nisi faʻamatalaga poʻo ia mautinoa e tuʻuina atu i matou imeli saʻo, matou te tali atu ia te oe i le na o le 24 itula faʻapea foi ma le upusii sili o le a tuʻuina atu.
Super Purchasing moSaina PCR, Real Time PCR, Faʻatasi ai ma le agaga faʻamalosi o le "maualuga maualuga, faigofie, faʻatinoga ma mea fou", ma e tusa ai ma ia taʻiala o le "lelei lelei ae sili atu le tau," ma le "aitalafu i le lalolagi atoa", o loʻo matou taumafai e galulue faʻatasi ma kamupani vaega taʻavale i le lalolagi atoa e faia se paaga manumalo-manumalo.