Serum(Plasma) miRNA Isolation Kit
Fa'amatalaga o pusa:
◆Seti atoa RNase-Free, e leai se mea e popole ai i le faʻaleagaina o le RNA.
◆DNA-Cleaning Column o lo'o pipii fa'apitoa ile DNA, ina ia mafai e le pusa ona aveese fa'ama'i genomic DNA e aunoa ma le fa'aopoopoina o le DNase fa'aopoopo.
◆RNA maualuga: RNA-na'o le Koluma ma fa'atusa tulaga ese e mafai ona fa'amama lelei le RNA.
◆Saosaoa vave: E faigofie le taotoga ma e mafai ona maeʻa i totonu ole 30 minute.
◆Saogalemu: E le mana'omia se su'ega reagent.
◆Tulaga maualuga: O vaega RNA ua maua mai e maualuga le mama, e leai se polotini ma isi mea leaga, ma e mafai ona faʻafeiloaʻi i le tele o faʻataʻitaʻiga faʻataʻitaʻiga.
Fa'amatalaga
O lenei pusa o loʻo faʻaaogaina le koluma vili ma fua faʻatatau e fausia e la matou kamupani, lea e mafai ona faʻaleleia lelei vaega laiti o le miRNA i lalo ifo o le 100nt mai le serum ma le plasma.O le pusa e tuʻuina atu ai le DNA-Cleaning Column, lea e mafai ona faigofie ona vavae ese le supernatant ma le tissue lysate ma faʻapipiʻi ma aveese le DNA i totonu o le faʻataʻitaʻiga, lea e faigofie ma faʻasaoina taimi;RNA-na'o le Column e mafai ona fusia lelei le RNA, ma fa'atasi ai ma se fua fa'atatau, e mafai ona fa'aoga i le taimi e tasi.Fa'agasolo le tele o fa'ata'ita'iga.
Ole faiga atoa ole RNase-Free, ina ia le fa'aleagaina le miRNA mama;Fa'apolopolo miBRW1 ma Buffer miBRW2 fa'amama fa'amama fa'apolopolo e maua ai le RNA e aunoa ma le polotini, DNA, ion, ma le filogia fa'aola.
Fa'amatalaga
5T, 50T, 100T, 200T
Vaega o pusa
| Serum/Plasma miRNA Isolation Kit | ||||
| Mea i totonu o pusa | RE-0111T | RE-01111 | RE-01112 | RE-01114 |
| 5 T | 50 T | 100T | 200T | |
| Puipui miBRL1* | 2ml | 20ml | 40ml | 80ml |
| Puipui miBRL2* | 1.2ml | 12ml | 24ml | 48ml |
| Puipui miBRW1 | 2ml | 20ml | 40ml | 80ml |
| Puipui miBRW2 | 2.4ml | 24ml | 48ml | 96ml |
| RNase-Free ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| RNA-na'o Koluma | 5 fasi | 50fasi | 100fasi | 200fasi |
| DNA-Cleaning Koluma | 5 fasi | 50fasi | 100fasi | 200fasi |
| IFU | 1 fasi | 1 fasi | 1 fasi | 1 fasi |
Ituaiga & itu lelei
◆Seti atoa RNase-Free, e leai se mea e popole ai i le faʻaleagaina o le RNA.
◆DNA-Cleaning Column o lo'o pipii fa'apitoa ile DNA, ina ia mafai e le pusa ona aveese fa'ama'i genomic DNA e aunoa ma le fa'aopoopoina o le DNase fa'aopoopo.
◆RNA maualuga: RNA-na'o le Koluma ma fa'atusa tulaga ese e mafai ona fa'amama lelei le RNA.
◆Saosaoa vave: E faigofie le taotoga ma e mafai ona maeʻa i totonu ole 30 minute.
◆Saogalemu: E le mana'omia se su'ega reagent.
◆Tulaga maualuga: O vaega RNA ua maua mai e maualuga le mama, e leai se polotini ma isi mea leaga, ma e mafai ona faʻafeiloaʻi i le tele o faʻataʻitaʻiga faʻataʻitaʻiga.
Fa'asologa o pusa
Talosaga Pusa
E talafeagai mo le aveeseina ma le faamamaina o le miRNA mai manu ma tagata serum ma plasma.
Talosaga miRNA
O le miRNA faʻamamāina e le Serum / Plasma miRNA Isolation Kit e mafai ona faʻaaogaina i faʻataʻitaʻiga mole mole i lalo ifo, e pei o le: RT-PCR, Real Time RT-PCR, Northern Blot, suʻesuʻega chip, ma isi.
Fa'agasologa o galuega
Tulaga teuina
O lenei pusa e mafai ona teuina mo le 12 masina i lalo ole tulaga mago ile vevela ole potu(15-25°C).If e tatau ona teuina mo se taimi umi,faʻamolemoleteuina i le 2-8°C.
