Matou te pipii pea i le mataupu faavae "Uiga Muamua, Faʻailoga Sili".Matou te tuuto atoatoa i le tuʻuina atu ia matou tagata faʻatau oloa faʻatauvaʻa lelei, tuʻuina vave ma auaunaga faʻapolofesa mo Pa'u masani.Saina Nucleic Acid Extraction Reagent(Serum/ Plasma ma Puncture solution) , Rna Extraction Test Kits, Matou te taulaʻi i le fausiaina o oe lava faʻailoga ma faʻatasi ma le tele o faʻamatalaga faʻapitoa ma meafaigaluega muamua.O a matou oloa e te taua ua ia te oe.
Matou te pipii pea i le mataupu faavae "Uiga Muamua, Faʻailoga Sili".Matou te tuuto atoatoa i le tuʻuina atu ia matou tagata faʻatau oloa faʻatauvaʻa lelei, vave tuʻuina atu ma auaunaga faʻapitoa moSaina Nucleic Acid Extraction Reagent, Metotia Bead maneta, O le tatou Kamupani faiga fa'avae o le "lelei muamua, ia sili atu ma malosi, atina'e gafataulimaina" .O a tatou sini tuliloaina o le "mo sosaiete, tagata faʻatau, tagata faigaluega, paaga ma atinaʻe e saili manuia talafeagai".Matou te faʻamoemoe e galulue faʻatasi ma mea eseese uma e gaosia vaega taʻavale, fale faʻaleleia, taʻavale taʻavale, ona fatuina lea o se lumanaʻi matagofie!Faafetai mo le faʻaaluina o le taimi e suʻesuʻe ai la matou upega tafaʻilagi ma matou te faʻafeiloaia soʻo se fautuaga e te maua e mafai ona fesoasoani ia i matou e faʻaleleia atili la matou upega tafaʻilagi.

Fa'amatalaga Pusa

50 Sauniuniga, 200 Sauniuniga

O lenei pusa o loʻo faʻaaogaina le koluma vili ma le fua faʻavae e fausia e la matou kamupani, lea e mafai ona maua mai ai le maualuga o le mama ma le maualuga o le RNA atoa mai meaola eseese o manu ma le maualuga maualuga. E maua ai se DNA-Cleaning Column lelei, lea e mafai ona faigofie ona vavae ese ma adsorb genomic DNA mai le supernatant ma le lysate tissue, faigofie ma sefe taimi;RNA-na'o le Column e mafai ona fusifusia lelei le RNA ma e mafai ona fa'agasolo fa'atasi ma se fua fa'apitoa Tele fa'ata'ita'iga.

O le faiga atoa e RNase-Free, ina ia le faʻaleagaina le RNA na maua mai;Buffer RW1, Buffer RW2 faʻapipiʻi fufulu faiga, ina ia maua le RNA e saʻoloto mai le polotini, DNA, ion, ma le faʻaleagaina o mea faʻalaʻau.

Vaega o pusa:

Fa'apitoa & itu lelei

■ Leai se mea e popole ai e uiga i le faaleagaina o le RNA;ole faiga atoa ole RNase-Free
■ Aveese lelei le DNA-faʻaaogaina le DNA-Cleaning Column
■ Aveese le DNA e aunoa ma le faaopoopoina o le DNase
■ Faigofie-o faagaioiga uma e faamaeaina i le vevela o le potu
■ Saosaoa -e mafai ona faamaeaina i le 30 minute
■ Saogalemu-e le mana'omia se reagent organic
■ maualuga mama -OD260/280≈1.8-2.1

Fa'aoga pusa

E talafeagai mo le aveeseina ma le faʻamamaina o le RNA atoa mai le tele o mea fou pe faʻaisa manu aano poʻo sela faʻaleaganuʻu.

Fa'atauga o oloa

■ Talosaga i lalo: fa'asologa muamua o le cDNA, RT-PCR, mole mole, Blot i Matu, ma isi.
■ Fa'ata'ita'iga: aano o manu, sela fa'atupuina
■ Fuafuaga: Tissue 10-20mg, Cells(2-5)×10⁶
■ Ole maualuga ole gafatia ole fusifusia ole DNA ole koluma fa'amamaina: 80 μg
■ Elution voluma: 50-200 μl

Fa'agasologa o galuega

Ata

Manu Aofa'i RNA Isolation Kit togafitia 20mg
Fa'ata'ita'iga o isumu fou, ave 5% fa'amama atoa RNA 1% agar

Glycogel electrophoresis
1: Spleen 2: Fatugao
3: Ate 4: Loto

Teuina ma le ola fa'asa

E mafai ona teu le pusa mo le 24 masina i le vevela o le potu (15-25 ℃) poʻo le 2-8 ℃ mo se taimi umi.E mafai ona teuina le Buffer RL1 i le 4 ℃ mo le 1 masina pe a uma ona fa'aopoopo le β-mercaptoethanol (filifiliga).

Matou te pipii pea i le mataupu faavae "Uiga Muamua, Faʻailoga Sili".Matou te tuuto atoatoa i le tuʻuina atu ia matou tagata faʻatau oloa faʻatauvaʻa, tuʻuina atu vave ma auaunaga faʻapolofesa mo Ordinary Discount China Nucleic Acid Extraction Reagent (Serum / Plasma ma Puncture solution), Rna Extraction Test Kits, Matou te taulaʻi i le fausiaina o latou lava faʻailoga ma faʻatasi ma le tele o faʻamatalaga poto masani ma meafaigaluega muamua.O a matou oloa e te taua ua ia te oe.
Pa'u masani Saina Nucleic Acid Extraction Reagent,Metotia Bead maneta, O le tatou Kamupani faiga fa'avae o le "lelei muamua, ia sili atu ma malosi, atina'e gafataulimaina" .O a tatou sini tuliloaina o le "mo sosaiete, tagata faʻatau, tagata faigaluega, paaga ma atinaʻe e saili manuia talafeagai".Matou te faʻamoemoe e galulue faʻatasi ma mea eseese uma e gaosia vaega taʻavale, fale faʻaleleia, taʻavale taʻavale, ona fatuina lea o se lumanaʻi matagofie!Faafetai mo le faʻaaluina o le taimi e suʻesuʻe ai la matou upega tafaʻilagi ma matou te faʻafeiloaia soʻo se fautuaga e te maua e mafai ona fesoasoani ia i matou e faʻaleleia atili la matou upega tafaʻilagi.