O loʻo i ai se uiga lelei ma alualu i luma i le fiafia o tagata faʻatau, o la matou atinaʻe e faʻaleleia atili a matou meataitasi e faʻamalieina manaʻoga o tagata faʻatau ma sili atu ona taulaʻi i le saogalemu, faʻatuatuaina, manaʻoga o le siosiomaga, ma le faʻafouina o le Manufacturer for China Magnetic Beads Nucleic Acid PCR Extraction Kit ma le CE mo Poct PCR Machine, Faʻamoemoe faʻamaoni o loʻo tatou tuputupu aʻe faʻatasi ma lo tatou siosiomaga.
O loʻo i ai se uiga lelei ma agaʻi i luma i le fiafia o tagata faʻatau, o la matou atinaʻe e faʻaleleia atili a matou meataitasi e faʻamalieina manaʻoga o tagata faʻatau ma faʻalautele atili i le saogalemu, faʻamaoni, manaoga o le siosiomaga, ma le faʻafouina oChina DNA Extraction Kit, Nucleic Acid Extraction Kit, Matou te tausisi i le tagata fa'atau 1st, pito i luga tulaga 1st, fa'aleleia atili, manuia fa'atasi ma ta'iala manumalo.Pe a galulue faʻatasi ma le tagata faʻatau, matou te tuʻuina atu tagata faʻatau ma le maualuga maualuga o le auaunaga.Faʻamauina sootaga lelei pisinisi e faʻaaoga ai le tagata faʻatau a Zimbabwe i totonu o le pisinisi, ua matou faʻatuina a matou lava igoa ma igoa.I le taimi lava e tasi, fa'afeiloa'i ma le loto atoa i avanoa fou ma tuai i la matou kamupani e o atu e fa'atalanoa pisinisi laiti.

Fa'amatalaga

O lenei pusa o loʻo faʻaaogaina se faiga faʻapipiʻi lysis faʻapitoa e mafai ona vave faʻasaʻo le RNA mai faʻataʻitaʻiga faʻaleaganuʻu mo le RT-qPCR tali, ma faʻaumatia ai le taimi faʻaalu ma le galue RNA faʻamamaina.E mafai ona maua le mamanu RNA ile na'o le 7 minute.O le 5×Direct RT Mix ma le 2×Direct qPCR Mix-SYBR reagents e saunia e le pusa e mafai ona vave ma lelei ona maua i'uga o le PCR fa'atusatusa i le taimi moni.

5 × Direct RT Mix ma 2 × Direct qPCR Mix-SYBR o loʻo i ai le faʻapalepale malosi o le inhibitor, ma o le lysate o faʻataʻitaʻiga e mafai ona faʻaaogaina e fai ma faʻataʻitaʻiga mo RT-qPCR saʻo.O lenei pusa o loʻo i ai le RNA maualuga-affinity Foregene reverse transcriptase, ma Hot D-Taq DNA polymerase, dNTPs, MgCl₂, tali fa'afefete, PCR optimizer ma fa'amautu.

Fa'amatalaga

200×20μl Rxns, 1000×20μl Rxns

Vaega o pusa

Fa'apitoa & itu lelei

■ Faigofie ma aoga : faʻatasi ai ma le Cell Direct RT tekinolosi, RNA faʻataʻitaʻiga e mafai ona maua i totonu o le 7 minute.

■ O le faʻataʻitaʻiga manaʻoga e laʻititi, e pei o le 10 sela e mafai ona suʻeina.

■ Tulaga maualuga: e mafai ona vave iloa le RNA i sela o loʻo faʻatupuina ile 384, 96, 24, 12, 6-well plates.

■ DNA Eraser e mafai ona vave aveese genomes tatala, faʻaitiitia le aʻafiaga i faʻataʻitaʻiga mulimuli ane.

■ Optimized RT ma qPCR system e sili atu ona lelei le fa'aliliuina o le RT-PCR la'asaga e lua ma sili atu ona patino le PCR, ma sili atu ona fa'asa'o ile fa'ainitaneti tali ole RT-qPCR.

Fa'aoga pusa

Avanoa o le faʻaogaina: sela faʻaleaganuʻu.

- RNA fa'amatuu mai ile fa'ata'ita'iga lysis: na'o le fa'ata'ita'iga RT-qPCR o lenei pusa.

- O le pusa e mafai ona faʻaoga mo faʻamoemoega nei: suʻesuʻega faʻamatalaga o kenera, faʻamaoniga o le siRNA-mediated gene silencing effect, suʻesuʻeina o fualaau, ma isi.

Ata

Teuina ma le ola fa'asao

Vaega I o lenei pusa e tatau ona teuina i le 4 ℃;Vaega II e tatau ona teuina ile -20℃.

Foregene Protease Plus II e tatau ona teuina i le 4℃, aua le fa'aisa ile -20℃.

Reagent 2×Direct qPCR Mix-SYBR e tatau ona teuina i le -20 ℃ i le pogisa;pe a faʻaaoga soo, e mafai foi ona teuina i le 4 ℃ mo le teuina mo taimi pupuu (faʻaaogaina i totonu o le 10 aso). O loʻo i ai se uiga lelei ma le alualu i luma i le fiafia o tagata faʻatau, o la matou atinaʻe e faʻaleleia pea a matou mea e sili ona lelei e faʻamalieina ai manaʻoga o tagata faʻatau ma sili atu ona taulaʻi i le saogalemu, faʻatuatuaina, manaʻoga o le siosiomaga, ma le faʻafouina o le Manufacturer mo China Nucletic Becidad Kit PC ma le faʻamoemoega o le PC Nucletic PC. o loʻo tatou tuputupu aʻe faʻatasi ma o tatou faʻamoemoega i luga o le siosiomaga.
Faumea moChina DNA Extraction Kit, Nucleic Acid Extraction Kit, Matou te tausisi i le tagata fa'atau 1st, pito i luga tulaga 1st, fa'aleleia atili, manuia fa'atasi ma ta'iala manumalo.Pe a galulue faʻatasi ma le tagata faʻatau, matou te tuʻuina atu tagata faʻatau ma le maualuga maualuga o le auaunaga.Faʻamauina sootaga lelei pisinisi e faʻaaoga ai le tagata faʻatau a Zimbabwe i totonu o le pisinisi, ua matou faʻatuina a matou lava igoa ma igoa.I le taimi lava e tasi, fa'afeiloa'i ma le loto atoa i avanoa fou ma tuai i la matou kamupani e o atu e fa'atalanoa pisinisi laiti.