Faʻaaogaina se polokalame faʻasaienisi maualuga maualuga pulega, maualuga maualuga maualuga ma faʻatuatua maualuga, matou te mauaina le igoa lelei ma nofoia lenei pisinisi moTulaga maualuga RNA, O la matou mataupu o le "Tau talafeagai, taimi gaosiga taugofie ma auaunaga sili ona lelei" Matou te faʻamoemoe e galulue faʻatasi ma le tele o tagata faʻatau mo le faʻaleleia atili ma faʻamanuiaga.
Faʻaaogaina se polokalame faʻasaienisi maualuga maualuga pulega, maualuga maualuga maualuga ma faʻatuatua maualuga, matou te mauaina le igoa lelei ma nofoia lenei pisinisi moTulaga maualuga RNA, O la matou kamupani e faʻatatau i "tau talafeagai, maualuga maualuga, taimi gaosiga lelei ma le lelei pe a uma ona faʻatau atu auaunaga" e pei o la matou mataupu.Matou te faʻamoemoe e galulue faʻatasi ma le tele o tagata faʻatau mo le atinaʻeina ma faʻamanuiaga i le lumanaʻi.Fa'afeiloa'i e fa'afeso'ota'i i matou.

Fa'amatalaga

50 Sauniuniga, 200 Sauniuniga

Vaega o pusa

Fa'apitoa & itu lelei

■ O le faagasologa atoa e faagaoioia i le vevela potu (15-25 ℃), e aunoa ma le taele aisa ma le centrifugation maualalo vevela.
■ Ole pusa atoa ole RNase-Free, e leai se mea e popole ai ile faʻaleagaina ole RNA.
■ DNA-Cleaning Column o lo'o fusifusia fa'apitoa ai le DNA, ina ia mafai e le pusa ona aveese fa'ama'i genomic DNA e aunoa ma le fa'aopoopoina o le DNase fa'aopoopo.
■ Mauaina RNA maualuga: RNA-na'o Koluma ma fua fa'atatau e mafai ona fa'amama lelei le RNA.
■ Saosaoa vave: faigofie ona faagaoioia ma mafai ona faamaeaina i le 11 minute.
■ Saogalemu: E le mana'omia se mea fa'aola.
■ Tulaga maualuga: O le RNA faʻamamaina e maualuga le mama, e leai se polotini ma isi mea leaga, ma e mafai ona faʻafeiloaʻi isi suʻega mulimuli ane.

Fa'aoga pusa

E talafeagai mo le aveeseina ma le faʻamamaina o le RNA atoa mai sela faʻaleaganuʻu ile 96, 24, 12, ma le 6-well plates.

Fa'agasologa o galuega

Ata

O le agarose gel ata ma'a o Cell Aofa'i RNA Isolation Kit togafitia le numera eseese o lo'o i luga, 20μl volume elution, ave 2μl faamamaina atoa RNA 1%.

Teuina ma le ola fa'asa

E mafai ona teu le pusa mo le 12 masina i le vevela o le potu (15–25 ℃) ma le 2–8 ℃ mo se taimi umi (24 masina).

Buffer crl1 mafai ona teuina i le 4 ℃ mo le 1 masina pe a uma ona faʻaopopoina le 2-hydroxy-1-ethanethom i luga o le sili atu o faʻasaopeʻaina ma penefiti.
Tulaga Maualuga mo Saina Nomilin, Solvent Extraction, O la matou kamupani e faʻatatau i "tau talafeagai, maualuga maualuga, taimi lelei gaosiga ma le lelei pe a uma ona faʻatau atu auaunaga" e pei o la matou mataupu.Matou te faʻamoemoe e galulue faʻatasi ma le tele o tagata faʻatau mo le atinaʻeina ma faʻamanuiaga i le lumanaʻi.Fa'afeiloa'i e fa'afeso'ota'i i matou.