Faatasi ai ma lenei mautauave i le mafaufau, ua matou liliu atu i se tasi e foliga mai e sili ona faʻatekonolosi fou, taugofie, ma tau-faʻatauvaʻa gaosi oloa mo le Tulaga Maualuga.Saina Nucleic Acid Extraction Reagent, Rna Extraction Test Kits Nucleic Acid Extraction Reagent, Faʻafeiloaʻi oe e mautinoa lava o se vaega o matou faʻatasi ma le tasi e faʻafaigofie lau faʻalapotopotoga.E masani lava ona avea i matou ma au paaga sili pe a e manaʻo e fai sau lava pisinisi laiti.
Faatasi ai ma lenei mautauave i le mafaufau, ua matou liliu atu i se tasi e foliga mai e sili ona faʻatekonolosi, taugofie, ma faʻatauvaʻa gaosi oloa mo.Saina Nucleic Acid Extraction Reagent, Metotia Bead maneta, O le a matou faia le mea sili matou te mafaia e galulue faʻatasi & faamalieina ma oe faʻalagolago i luga o le tulaga maualuga ma le tau faʻatauvaʻa ma sili ona lelei pe a uma le tautua, faʻamoemoe ma le faʻamaoni e galulue faʻatasi ma oe ma faia taunuʻuga i le lumanaʻi!

Fa'amatalaga

50 Sauniuniga, 200 Sauniuniga

O lenei pusa o loʻo faʻaogaina le koluma vili ma fua faʻatatau na fausia e Foregene, lea e mafai ona maua lelei le RNA maualuga-mama ma maualuga maualuga mai sela na faʻatupuina ile 96, 24, 12, ma le 6-well plates.

O le pusa e maua ai se DNA-Cleaning Column lelei, lea e faigofie ona tu'u'ese'ese le supernatant ma le cell lysate, fusifusia ma aveese genomic DNA.O le taotoga e faigofie ma sefe taimi.

O le RNA-na'o le Column e mafai ona fa'amauina lelei le RNA i se fua fa'atatau.O se numera tele o faʻataʻitaʻiga e mafai ona faʻatautaia i le taimi e tasi.

Vaega o pusa

*Fa'amolemole fai totigilima ma fai ni puipuiga i le taimi o le ta'otoga aua o le Buffer cRL1 ma le Buffer RW1 o lo'o i ai masima fa'afefete.

Fa'apitoa & itu lelei

■ O le faagasologa atoa e faagaoioia i le vevela potu (15-25 ℃), e aunoa ma le taele aisa ma le centrifugation maualalo vevela.
■ Ole pusa atoa ole RNase-Free, e leai se mea e popole ai ile faʻaleagaina ole RNA.
■ DNA-Cleaning Column o lo'o fusifusia fa'apitoa ai le DNA, ina ia mafai e le pusa ona aveese fa'ama'i genomic DNA e aunoa ma le fa'aopoopoina o le DNase fa'aopoopo.
■ Mauaina RNA maualuga: RNA-na'o Koluma ma fua fa'atatau e mafai ona fa'amama lelei le RNA.
■ Saosaoa vave: faigofie ona faagaoioia ma mafai ona faamaeaina i le 11 minute.
■ Saogalemu: E le mana'omia se mea fa'aola.
■ Tulaga maualuga: O le RNA faʻamamaina e maualuga le mama, e leai se polotini ma isi mea leaga, ma e mafai ona faʻafeiloaʻi isi suʻega mulimuli ane.

Fa'aoga pusa

E talafeagai mo le aveeseina ma le faʻamamaina o le RNA atoa mai sela faʻaleaganuʻu ile 96, 24, 12, ma le 6-well plates.

Fa'agasologa o galuega

Ata

O le agarose gel ata ma'a o Cell Aofa'i RNA Isolation Kit togafitia le numera eseese o lo'o i luga, 20μl volume elution, ave 2μl faamamaina atoa RNA 1%.

Teuina ma le ola fa'asa

E mafai ona teu le pusa mo le 12 masina i le vevela o le potu (15-25 ℃) poʻo le 2-8 ℃ mo se taimi umi (24 masina).

E mafai ona teuina le Buffer cRL1 i le 4 ℃ mo le 1 masina pe a uma ona faʻaopopo le 2-hydroxy-1-ethanethiol (filifiliga). Faatasi ai ma lenei mautauave i le mafaufau, ua matou liliu atu i se tasi o mea sili ona fou faʻatekonolosi, taugofie, ma tau-faʻatauvaʻa gaosi oloa mo High QualitySaina Nucleic Acid Extraction Reagent, Rna Extraction Test Kits Nucleic Acid Extraction Reagent, Faʻafeiloaʻi oe e mautinoa lava o se vaega o matou faʻatasi ma le tasi e faʻafaigofie lau faʻalapotopotoga.E masani lava ona avea i matou ma au paaga sili pe a e manaʻo e fai sau lava pisinisi laiti.
High Quality China Nucleic Acid Extraction Reagent,Metotia Bead maneta, O le a matou faia le mea sili matou te mafaia e galulue faʻatasi & faamalieina ma oe faʻalagolago i luga o le tulaga maualuga ma le tau faʻatauvaʻa ma sili ona lelei pe a uma le tautua, faʻamoemoe ma le faʻamaoni e galulue faʻatasi ma oe ma faia taunuʻuga i le lumanaʻi!