O la tatou taulaiga e tatau ona faʻamalosia ma faʻaleleia le lelei ma le toe faʻaleleia o oloa o loʻo i ai nei, i le taimi nei ia faʻatutuina pea oloa fou e faʻafetaui ai tagata faʻatau ' manaʻomia mo le lelei lelei China Column Nucleic Acid Rna Isolation Kit, lona tolu o augatupulaga, O manatu taʻitasi ma fuafuaga o le a matua talisapaia!O le felagolagomai lelei e mafai ona faʻaleleia atili ai i maʻua uma i le tuputupu aʻe sili atu!
O la matou taulaiga e tatau ona faʻamalosia ma faʻaleleia le lelei ma le toe faʻaleleia o oloa o loʻo iai nei, i le taimi nei e faʻavae i taimi uma oloa fou e faʻafetaui ai tagata faʻatau tulaga manaʻomia moPusa Faaesea Saina, Pcr Rna Su'ega Su'e, Matou te pipii atu i le faamaoni, lelei, aoga manumalo-manumalo tamoe misiona ma tagata-faatatau pisinisi filosofia.Tulaga lelei, tau talafeagai ma le faamalieina o tagata faatau e tuliloaina pea!Afai e te fiafia i a matou mea, taumafai e faʻafesoʻotaʻi i matou mo nisi faʻamatalaga!

Fa'amatalaga

50 Sauniuniga, 200 Sauniuniga

Vaega o pusa

Fa'apitoa & itu lelei

■ Leai se mea e popole ai e uiga i le faaleagaina o le RNA;ole faiga atoa ole RNase-Free
■ Aveese lelei le DNA-faʻaaogaina le DNA-Cleaning Column
■ Aveese le DNA e aunoa ma le faaopoopoina o le DNase
■ Faigofie-o faagaioiga uma e faamaeaina i le vevela o le potu
■ Saosaoa -e mafai ona faamaeaina i le 30 minute
■ Saogalemu-e le mana'omia se reagent organic
■ maualuga mama -OD260/280≈1.8-2.1

Fa'aoga pusa

E talafeagai mo le aveeseina ma le faʻamamaina o le RNA atoa mai le tele o mea fou pe faʻaisa manu aano poʻo sela faʻaleaganuʻu.

Fa'atauga o oloa

■ Talosaga i lalo: fa'asologa muamua o le cDNA, RT-PCR, mole mole, Blot i Matu, ma isi.
■ Fa'ata'ita'iga: aano o manu, sela fa'atupuina
■ Fuafuaga: Tissue 10-20mg, Cells(2-5)×10⁶
■ Ole maualuga ole gafatia ole fusifusia ole DNA ole koluma fa'amamaina: 80 μg
■ Elution voluma: 50-200 μl

Fa'agasologa o galuega

Ata

Manu Aofa'i RNA Isolation Kit togafitia 20mg
Fa'ata'ita'iga o isumu fou, ave 5% fa'amama atoa RNA 1% agar

Glycogel electrophoresis
1: Spleen 2: Fatugao
3: Ate 4: Loto

Teuina ma le ola fa'asa

E mafai ona teu le pusa mo le 24 masina i le vevela o le potu (15-25 ℃) poʻo le 2-8 ℃ mo se taimi umi.E mafai ona teuina le Buffer RL1 i le 4 ℃ mo le 1 masina pe a uma ona fa'aopoopo le β-mercaptoethanol (filifiliga).

O la tatou taulaiga e tatau ona faʻamalosia ma faʻaleleia le tulaga lelei ma le toe faʻaleleia o oloa o loʻo i ai nei, i le taimi nei e faʻavae i taimi uma ni oloa fou e faʻafeiloaʻi ai manaoga faʻapitoa a tagata faʻatau mo le lelei lelei China Magnetic Particles Nucleic Acid Rna Isolation Kit, O manatu taʻitasi ma fuafuaga o le a matua talisapaia!O le felagolagomai lelei e mafai ona faʻaleleia atili ai i maʻua uma i le tuputupu aʻe sili atu!
Lelei leleiPusa Faaesea Saina, PCR Rna Extraction Reagent, Matou te pipii atu i le faamaoni, lelei, aoga manumalo-manumalo misiona ma filosofia pisinisi faʻatatau i tagata.Tulaga lelei, tau talafeagai ma le faamalieina o tagata faatau e tuliloaina pea!Afai e te fiafia i a matou mea, taumafai e faʻafesoʻotaʻi i matou mo nisi faʻamatalaga!