Fa'amanino le "Fa'atau muamua, Sili muamua" i le mafaufau, matou te galulue vavalalata ma a matou tagata fa'atau ma tu'uina atu ia i latou auaunaga lelei ma fa'apitoa mo Falefa'atau oloa si'iatoa.Saina Nucleic Acid Extraction ReagentRna Extraction Test Kits, O le matou kamupani e tu'ufa'atasia e ofoina atu i tagata fa'atau mea tetele ma malupuipuia i le tau fa'atauva'a, ma maua ai tagata uma e fa'amalieina a matou tautua.
Fa'amanino "Fa'atau muamua, Sili muamua" i lou mafaufau, matou te galulue vavalalata ma a matou tagata fa'atau ma tu'uina atu ia i latou auaunaga lelei ma fa'apitoa moSaina Nucleic Acid Extraction Reagent, O a matou mea e faʻaalia lautele ma faʻatuatuaina e tagata faʻaoga ma e mafai ona faʻafeiloaʻi faʻaauau le atinaʻeina o manaʻoga tau tamaoaiga ma agafesootai.Matou te faʻafeiloaʻi tagata fou ma tagata tuai mai soʻo se ituaiga olaga e faʻafesoʻotaʻi i matou mo mafutaga pisinisi i le lumanaʻi ma ausia le manuia faʻatasi!

Fa'amatalaga

50 Sauniuniga, 200 Sauniuniga

O lenei pusa o loʻo faʻaogaina le koluma vili ma fua faʻatatau na fausia e Foregene, lea e mafai ona maua lelei le RNA maualuga-mama ma maualuga maualuga mai sela na faʻatupuina ile 96, 24, 12, ma le 6-well plates.

O le pusa e maua ai se DNA-Cleaning Column lelei, lea e faigofie ona tu'u'ese'ese le supernatant ma le cell lysate, fusifusia ma aveese genomic DNA.O le taotoga e faigofie ma sefe taimi.

O le RNA-na'o le Column e mafai ona fa'amauina lelei le RNA i se fua fa'atatau.O se numera tele o faʻataʻitaʻiga e mafai ona faʻatautaia i le taimi e tasi.

Vaega o pusa

*Fa'amolemole fai totigilima ma fai ni puipuiga i le taimi o le ta'otoga aua o le Buffer cRL1 ma le Buffer RW1 o lo'o i ai masima fa'afefete.

Fa'apitoa & itu lelei

■ O le faagasologa atoa e faagaoioia i le vevela potu (15-25 ℃), e aunoa ma le taele aisa ma le centrifugation maualalo vevela.
■ Ole pusa atoa ole RNase-Free, e leai se mea e popole ai ile faʻaleagaina ole RNA.
■ DNA-Cleaning Column o lo'o fusifusia fa'apitoa ai le DNA, ina ia mafai e le pusa ona aveese fa'ama'i genomic DNA e aunoa ma le fa'aopoopoina o le DNase fa'aopoopo.
■ Mauaina RNA maualuga: RNA-na'o Koluma ma fua fa'atatau e mafai ona fa'amama lelei le RNA.
■ Saosaoa vave: faigofie ona faagaoioia ma mafai ona faamaeaina i le 11 minute.
■ Saogalemu: E le mana'omia se mea fa'aola.
■ Tulaga maualuga: O le RNA faʻamamaina e maualuga le mama, e leai se polotini ma isi mea leaga, ma e mafai ona faʻafeiloaʻi isi suʻega mulimuli ane.

Fa'aoga pusa

E talafeagai mo le aveeseina ma le faʻamamaina o le RNA atoa mai sela faʻaleaganuʻu ile 96, 24, 12, ma le 6-well plates.

Fa'agasologa o galuega

Ata

O le agarose gel ata ma'a o Cell Aofa'i RNA Isolation Kit togafitia le numera eseese o lo'o i luga, 20μl volume elution, ave 2μl faamamaina atoa RNA 1%.

Teuina ma le ola fa'asa

E mafai ona teu le pusa mo le 12 masina i le vevela o le potu (15-25 ℃) poʻo le 2-8 ℃ mo se taimi umi (24 masina).

Buffer cRL1 e mafai ona teuina i le 4 ℃ mo le 1 masina pe a uma ona faaopoopo 2-hydroxy-1-ethanethiol (filifiliga). Faʻamanino le "Faʻatau muamua, Sili muamua" i le mafaufau, matou te galulue vavalalata ma a matou tagata faʻatau ma tuʻuina atu ia i latou auaunaga lelei ma faʻapitoa mo Factory wholesale China Nucleic Acid Extraction Reagent (Serum / Plasma and Puncture Suʻega o loʻo tuʻuina atu i luga o loʻo tuʻuina atu i luga o le Suʻega, Suʻega faʻapipiʻi ma le Puncture faʻatau) mea lelei i tau fa'atauvaga, maua ai tagata fa'atau uma e fa'amalieina i a tatou tautua.
Fale gaosi oloa siiatoa Saina Nucleic Acid Extraction Reagent, Magnetic Bead Method, O matou mea e lauiloa lautele ma faʻatuatuaina e tagata faʻaoga ma e mafai ona faʻafetaui le faʻaauauina o le atinaʻeina o manaʻoga tau tamaoaiga ma agafesootai.Matou te faʻafeiloaʻi tagata fou ma tagata tuai mai soʻo se ituaiga olaga e faʻafesoʻotaʻi i matou mo mafutaga pisinisi i le lumanaʻi ma ausia le manuia faʻatasi!