Sapalai Falegaosi Saina 2 X Vave Pfus PCR Master Mix ma le Pfu DNA Polymerase Dntps PCR Buffer Manufacturer and Supplier |Foregene

Sapalai Falegaosi 2 X Vave Pfus PCR Master Mix ma le Pfu DNA Polymerase Dntps PCR Buffer

Fa'amatalaga o pusa:

Fa'aaoga sa'o le polysaccharide ma le polyphenol leaf lysate e fai ma fa'ata'ita'iga mo le fa'alauteleina o le PCR, e aunoa ma le fa'aesea o le DNA, vave ma maualuga le lagona.

◮Leai se taimi faʻaalu ma taugata faʻamamaina DNA (Taimi, galuega ma tau faʻasaoina!)

◮itiiti meafaitino

◮Faigofie-o le faʻataʻitaʻiga e mafai ona tuʻuina atu i le PCR reaction poʻo le lysis reaction e aunoa ma le tipiina pe olo

◮2 X Faafefiloi—faaitiitia o mea sese o le utaina o faataitaiga ma le taimi o sauniuniga faiga faiga

◮Anapogi—o le tapenaga o ata e mafai ona mae'a ile 10 minute, ole PCR e mafai ona mae'a ile 50 minute

◮E mafai ona fa'amae'aina le fa'aogaina o le lysis i le 96-well PCR plate

Auina imeli ia matou La'u mai ile PDF

Fa'amatalaga Oloa

Faailoga o oloa

Matou te faʻamamafa le alualu i luma ma faʻafeiloaʻi oloa fou i le maketi i tausaga taʻitasi mo Factory Supply 2 X Fast Pfus PCR Master Mix with Pfu DNA Polymerase Dntps PCR Buffer, Aʻo matou agai i luma, matou te vaʻavaʻai atu i a matou oloa faʻalauteleina ma faʻaleleia atili a matou kamupani.
Matou te faʻamamafa le alualu i luma ma faʻalauiloa oloa fou i le maketi i tausaga taʻitasi moSaina PCR Master Mix ma PCR Reagent, Matou te faʻamoemoe e avea ma atinaʻe faʻaonaponei ma le tulaga faʻapisinisi o le "Faʻamaoni ma le mautinoa" ma le faʻamoemoe o le "Ofo atu i tagata faʻatau auaunaga sili ona faamaoni ma oloa sili ona lelei".Matou te talosaga atu ma le faamaoni mo lau lagolago e le suia ma talisapaia lau fautuaga agalelei ma le taitaiga.

Fa'amatalaga

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Vaega o pusa

Vaega I	Puipui PS1
	Puipui P2
	6 × DNA Loading Buffer
Vaega II	Puipui PS2
Vaega II	2× Laulau PCR Faigofie^TMFaafefiloi

Fa'apitoa & itu lelei

■ Leai se taimi e alu ai ma taugata DNA fa'amamāina
■ Laiti meafaitino
■ Faigofie-o le faʻataʻitaʻiga e mafai ona tuʻuina atu i le PCR reaction poʻo le lysis reaction e aunoa ma le tipiina pe olo
■ 2 × Faafefiloi, faʻaitiitia faʻataʻitaʻiga faʻapipiʻiina mea sese ma tali faiga taimi sauniuni
■ E mafai ona fa'amae'a le fa'ata'ita'iga fa'anatinati i le 10 minute, e mafai ona fa'amae'a le tali PCR i totonu ole 50 minute.
■ E mafai ona fa'amae'a le tali atu i le fa'aogaina-lysis maualuga ile 96-well PCR plate

Fa'asologa o pusa

Fa'aoga: Fa'ailoaina o la'au transgenic, genotyping, etc.

Fa'ata'ita'iga: lau la'au e mauoa i le polysaccharide po'o le polyphenol

Fa'ata'ita'iga tele: Diameter 2-3mm (auala tuusao), diameter 5-7mm (lysis method)

Su'esu'ega va'aiga: PCR oloa ≤1kb

Fa'agasologa o galuega

la'au la'au sa'o PCR fa'aopoopo-faigaluega

Electropherogram

lau la'au sa'o PCR fa'aopoopo Matou te faʻamamafa le alualu i luma ma faʻafeiloaʻi oloa fou i le maketi i tausaga taʻitasi mo Factory Supply 2 X Fast Pfus PCR Master Mix with Pfu DNA Polymerase Dntps PCR Buffer, Aʻo matou agai i luma, matou te vaʻavaʻai atu i a matou oloa faʻalauteleina ma faʻaleleia atili a matou kamupani.
Sapalai FalegaosiSaina PCR Master Mix ma PCR Reagent, Matou te faʻamoemoe e avea ma atinaʻe faʻaonaponei ma le tulaga faʻapisinisi o le "Faʻamaoni ma le mautinoa" ma le faʻamoemoe o le "Ofo atu i tagata faʻatau auaunaga sili ona faamaoni ma oloa sili ona lelei".Matou te talosaga atu ma le faamaoni mo lau lagolago e le suia ma talisapaia lau fautuaga agalelei ma le taitaiga.