Falegaosimea tu'uina atu Saina Fa'apolofesa Gaosi Fa'afoma'i PCR Su'ega Tau Taimi moni PCR masini mo Fa'atau
O la matou faʻalapotopotoga o loʻo pipii atu i lau mataupu faavae o le "Uiga e mafai ona avea ma ola o lau faʻalapotopotoga, ma o le igoa o le a avea ma agaga o ia mea" mo Fale gaosi oloa faʻapolofesa Saina Manufacturer Fomai PCR Suʻega Tau Taimi Moni PCR Machine mo Faʻatau, Faatasi ai ma se lautele lautele, tulaga maualuga, totogi taliaina ma fesoasoani tele, o le a avea i matou ma au paaga pisinisi sili.Matou te faʻafeiloaʻi faʻamoemoega fou ma muamua mai soʻo se ituaiga olaga e faʻafesoʻotaʻi ma i matou mo fegalegaleaiga pisinisi laiti o loʻo lumanaʻi ma faʻataunuʻuina ausiga tutusa!
O la matou faʻalapotopotoga o loʻo pipiimau i lau mataupu faavae o le "O le tulaga lelei atonu o le olaga o lau faʻalapotopotoga, ma o le igoa o le a avea ma agaga o ia" moOtometi Su'esu'ega Nucleic Acid, Saina Rt PCR Meafaigaluega Su'esu'e, O le peresetene ma sui uma o le kamupani e manaʻo e tuʻuina atu oloa faʻapitoa ma auaunaga mo tagata faʻatau ma faʻafeiloaʻi ma le faʻamaoni ma galulue faʻatasi ma tagata faʻatau uma ma fafo mo se lumanaʻi lumanaʻi.
Fa'amatalaga Pusa
50 Sauniuniga, 200 Sauniuniga
O lenei pusa o loʻo faʻaaogaina le koluma vili ma le fua faʻavae e fausia e la matou kamupani, lea e mafai ona maua mai ai le maualuga o le mama ma le maualuga o le RNA atoa mai meaola eseese o manu ma le maualuga maualuga. E maua ai se DNA-Cleaning Column lelei, lea e mafai ona faigofie ona vavae ese ma adsorb genomic DNA mai le supernatant ma le lysate tissue, faigofie ma sefe taimi;RNA-na'o le Column e mafai ona fusifusia lelei le RNA ma e mafai ona fa'agasolo fa'atasi ma se fua fa'apitoa Tele fa'ata'ita'iga.
O le faiga atoa e RNase-Free, ina ia le faʻaleagaina le RNA na maua mai;Buffer RW1, Buffer RW2 faʻapipiʻi fufulu faiga, ina ia maua le RNA e saʻoloto mai le polotini, DNA, ion, ma le faʻaleagaina o mea faʻalaʻau.
Vaega o pusa:
Puipui RL1 , Puipui RL2 |
Puipui RW1, Puipui RW2 |
RNase-Free ddH2O, Koluma Fa'amama DNA |
RNA-Na'o Koluma |
Fa'apitoa & itu lelei
■ Leai se mea e popole ai e uiga i le faaleagaina o le RNA;ole faiga atoa ole RNase-Free
■ Aveese lelei le DNA-faʻaaogaina le DNA-Cleaning Column
■ Aveese le DNA e aunoa ma le faaopoopoina o le DNase
■ Faigofie-o faagaioiga uma e faamaeaina i le vevela o le potu
■ Saosaoa -e mafai ona faamaeaina i le 30 minute
■ Saogalemu-e le mana'omia se reagent organic
■ maualuga mama -OD260/280≈1.8-2.1
Fa'aoga pusa
E talafeagai mo le aveeseina ma le faʻamamaina o le RNA atoa mai le tele o mea fou pe faʻaisa manu aano poʻo sela faʻaleaganuʻu.
Fa'atauga o oloa
■ Talosaga i lalo: fa'asologa muamua o le cDNA, RT-PCR, mole mole, Blot i Matu, ma isi.
■ Fa'ata'ita'iga: aano o manu, sela fa'atupuina
■ Fuafuaga: Tissue 10-20mg, Cells(2-5)×106
■ Ole maualuga ole gafatia ole fusifusia ole DNA ole koluma fa'amamaina: 80 μg
■ Elution voluma: 50-200 μl
Fa'agasologa o galuega
Ata
Manu Aofa'i RNA Isolation Kit togafitia 20mg
Fa'ata'ita'iga o isumu fou, ave 5% fa'amama atoa RNA 1% agar
Glycogel electrophoresis
1: Spleen 2: Fatugao
3: Ate 4: Loto
Teuina ma le ola fa'asa
E mafai ona teu le pusa mo le 24 masina i le vevela o le potu (15-25 ℃) poʻo le 2-8 ℃ mo se taimi umi.E mafai ona teuina le Buffer RL1 i le 4 ℃ mo le 1 masina pe a uma ona fa'aopoopo le β-mercaptoethanol (filifiliga).
O la matou faʻalapotopotoga o loʻo pipii atu i lau mataupu faavae o le "Uiga e mafai ona avea ma ola o lau faʻalapotopotoga, ma o le igoa o le a avea ma agaga o ia mea" mo Fale gaosi oloa faʻapolofesa Saina Manufacturer Fomai PCR Suʻega Tau Taimi Moni PCR Machine mo Faʻatau, Faatasi ai ma se lautele lautele, tulaga maualuga, totogi taliaina ma fesoasoani tele, o le a avea i matou ma au paaga pisinisi sili.Matou te faʻafeiloaʻi faʻamoemoega fou ma muamua mai soʻo se ituaiga olaga e faʻafesoʻotaʻi ma i matou mo fegalegaleaiga pisinisi laiti o loʻo lumanaʻi ma faʻataunuʻuina ausiga tutusa!
Falegaosimea sapalaiSaina Rt PCR Meafaigaluega Su'esu'e, Otometi Su'esu'ega Nucleic Acid, O le peresetene ma sui uma o le kamupani e manaʻo e tuʻuina atu oloa faʻapitoa ma auaunaga mo tagata faʻatau ma faʻafeiloaʻi ma le faʻamaoni ma galulue faʻatasi ma tagata faʻatau uma ma fafo mo se lumanaʻi lumanaʻi.