Falegaosi mea'ai 0.2ml Flat Cap Transparent PCR Tube
Fa'amatalaga o pusa:
◮O se inhibitor RNase fou e maua mai i le isumu o lo'o fa'aalia i siama fa'ainisinia E. coli e fa'aaoga ai le fa'aogaina o le fa'aogaina o le gene.O le inhibitor e taofia ai le gaioiga a le RNase e ala i le le faʻatauvaʻa tuʻufaʻatasia ma le RNase 1: 1, ma puipuia ai.RNAamiosa'o , ma e mafai ona fa'alavelave leleio leRNase A, B, C gaoioiga, ae le o RNase T1, T2, H, ma isi; E mafai ona toe fa'aliliuina o le fusifusia o RNase Inhibitor ma RNase, ma le lavelave e mafai ona vavaeeseina e le urea ma le sulfhydryl reagents, ina ia toe faʻafouina le RNase ma le inhibitor e le mafai ona faʻaaogaina.
Matou te faʻalagolago i mafaufauga faʻapitoa, faʻaonaponei faifaipea i vaega uma, alualu i luma tekinolosi ma o le mea moni i luga o matou tagata faigaluega o loʻo auai saʻo i la matou manuia mo Factory tuʻuina atu 0.2ml Flat Cap Transparent PCR Tube, Matou te faʻafeiloaia ma le fiafia lou auai e faʻalagolago i faʻamanuiaga faʻaopoopo i totonu o le latalata i le lumanaʻi.
Matou te faʻalagolago i mafaufauga faʻapitoa, faʻaonaponei faifaipea i vaega uma, alualu i luma tekinolosi ma o le mea moni i luga o matou tagata faigaluega e auai saʻo i lo matou manuia moSaina PCR Tube ma 0.2ml PCR Tube, O a tatou fofo e masani lava ona auina atu i fafo i saute-sasaʻe Asia Euro-Amerika, ma faʻatau atu i lo tatou atunuu uma.Ma faʻalagolago i tulaga sili ona lelei, tau talafeagai, auaunaga sili ona lelei, matou maua tali lelei mai tagata faʻatau i fafo.Fa'afeiloa'i oe e fa'atasi ma matou mo nisi avanoa ma fa'amanuiaga.Matou te faʻafeiloaʻi tagata faʻatau, faʻalapotopotoga faʻapisinisi ma uo mai itu uma o le lalolagi e faʻafesoʻotaʻi i matou ma saili le felagolagomaʻi mo manuia tutusa.
Fa'amatalaga
5ml, 10ml, 40ml
O le PCR Hero (Fa'atasi ma le Fa'a) Pusa fa'aaogā e aofia ai le tupulaga fou tulaga ese a Foregene o Taq—Foregene Taq Hero, dNTPs sili ona lelei, MgCl2, fa'afefe tali, fa'aleleia o tali PCR, fa'asili ma fa'amautu.O le 2 × PCR HeroTM Mix (lanu) faiga e maualuga atu le faapalepale i PCR inhibitors nai lo le PCR Mix masani faiga, ma e mafai ona faigofie ona taulimaina PCR amplification o mamanu lavelave eseese.O le faiga faʻapitoa faʻaalia ma le maualuga o le Taq Hero e faʻaalia ai le tali a le PCR e sili atu le faʻaleleia atili, faʻapitoa ma le maaleale.
Vaega o pusa
| 0.1U Taq Hero/μl |
| 400μM dNTPs |
| 20mM Tris-HCl(pH8.8) |
| 100mM KCl |
| 5mM MgCl2 |
| O isi fa'amautu ma fa'aleleia |
| DNA Loading Buffer |
Fa'apitoa & itu lelei
■ Faʻamaoni maualuga: 6 taimi nai lo le enzyme masani Taq;
■ Faʻateleina faʻavavevave faʻavavevave : o le saoasaoa faʻateleina o le 5-10sec / kb, lea e 6-12 taimi o le enzyme masani Taq;
■ E sili atu le fetuutuuna'i o mamanu: E mafai ona fa'atuputeleina fa'ata'ita'iga DNA lavelave eseese ma maualugaGC anotusi;
■ E sili atu le lelei o le fa'atuputeleina: ta'amilosaga fa'aola maualalo ifo nai lo le taq enzyme masani;
■ E sili atu le malosi o le faʻapalepale o le siosiomaga: tuʻu i le 37 ° C mo le vaiaso, faʻatumauina le sili atu i le 90% gaioiga;
■ E iai le 5′ →3′ DNA polymerase activity ma le 5′→3′ exonuclease activity, e aunoa ma le 3′ →5′ exonuclease activity.
■ O le pusa o loʻo muamua faʻafefiloi ma le Electrophoresis Loading Buffer, pe a maeʻa le PCR, e mafai ona uta saʻo le faʻataʻitaʻiga mo le electrophoresis;e faigofie ma faigofie le taotoga.
Fa'aoga pusa
PCR fa'ateleina o vaega DNA, fa'ailoga DNA, fa'asologa o DNA, PCR fa'atasi ma le si'usi'u
Teuina ma le ola fa'asao
E mafai ona teuina mo se taimi umi ile -20°C;mo le faʻaaogaina faifaipea, e mafai ona teuina i le 4 ° C mo sina taimi puupuu. Matou te faʻalagolago i mafaufauga faʻapitoa, faʻaonaponei faifaipea i vaega uma, alualu i luma tekinolosi ma o le mea moni i luga o matou tagata faigaluega o loʻo auai saʻo i lo matou manuia mo Factory tuʻuina atu 0.2ml Flat Cap Transparent PCR Tube, Matou te faʻafeiloaia ma le fiafia lou auai faʻalagolago i faʻamanuiaga faʻaopoopo i totonu o le lata mai.
Falegaosimea sapalaiSaina PCR Tube ma 0.2ml PCR Tube, O a tatou fofo e masani lava ona auina atu i fafo i saute-sasaʻe Asia Euro-Amerika, ma faʻatau atu i lo tatou atunuu uma.Ma faʻalagolago i tulaga sili ona lelei, tau talafeagai, auaunaga sili ona lelei, matou maua tali lelei mai tagata faʻatau i fafo.Fa'afeiloa'i oe e fa'atasi ma matou mo nisi avanoa ma fa'amanuiaga.Matou te faʻafeiloaʻi tagata faʻatau, faʻalapotopotoga faʻapisinisi ma uo mai itu uma o le lalolagi e faʻafesoʻotaʻi i matou ma saili le felagolagomaʻi mo manuia tutusa.
