O lo'o iai le matou tagata fa'apitoa, mataalia e tu'uina atu auaunaga maualuga mo le matou fa'atau.Matou te mulimuli i taimi uma i le faʻatonuga o tagata faʻatau, faʻamatalaga-taulai mo Fale Faʻatau Saina CE Faʻamaonia Nucleic Acid 64 Well Plate DNA Rna Extraction Isolation Kit mo PCR Virus Rapid Diagnostic, Matou te faʻafeiloaia oe e te faʻatasi ma i matou i lenei ala o le fatuina o se pisinisi manuia ma lelei faʻatasi.
O lo'o iai le matou tagata fa'apitoa, mataalia e tu'uina atu auaunaga maualuga mo le matou fa'atau.Matou te mulimuli i taimi uma i le aʻoaʻoga o tagata faʻatau, faʻamatalaga-taulai moSaina Viral Nucleic Acid Isolation, Su'esu'ega PCR Taimi Moni, “Ia sili atu ona aulelei tamaitai” o la matou filosofia faatau."O le avea ma tagata fa'atau oloa fa'atuatuaina ma fa'atauaina fa'atau oloa" o le sini lea a le matou kamupani.Matou te faʻamaoni i vaega uma o la matou galuega.Matou te faʻafeiloaʻi ma le faʻamaoni uo e faʻatalanoa pisinisi ma amata galulue faʻatasi.Matou te faʻamoemoe e faʻatasi lima ma uo i pisinisi eseese e fausia ai se lumanaʻi manuia.

Fa'amatalaga Pusa

50 Sauniuniga, 200 Sauniuniga

O lenei pusa o loʻo faʻaaogaina le koluma vili ma le fua faʻavae e fausia e la matou kamupani, lea e mafai ona maua mai ai le maualuga o le mama ma le maualuga o le RNA atoa mai meaola eseese o manu ma le maualuga maualuga. E maua ai se DNA-Cleaning Column lelei, lea e mafai ona faigofie ona vavae ese ma adsorb genomic DNA mai le supernatant ma le lysate tissue, faigofie ma sefe taimi;RNA-na'o le Column e mafai ona fusifusia lelei le RNA ma e mafai ona fa'agasolo fa'atasi ma se fua fa'apitoa Tele fa'ata'ita'iga.

O le faiga atoa e RNase-Free, ina ia le faʻaleagaina le RNA na maua mai;Buffer RW1, Buffer RW2 faʻapipiʻi fufulu faiga, ina ia maua le RNA e saʻoloto mai le polotini, DNA, ion, ma le faʻaleagaina o mea faʻalaʻau.

Vaega o pusa:

Fa'apitoa & itu lelei

■ Leai se mea e popole ai e uiga i le faaleagaina o le RNA;ole faiga atoa ole RNase-Free
■ Aveese lelei le DNA-faʻaaogaina le DNA-Cleaning Column
■ Aveese le DNA e aunoa ma le faaopoopoina o le DNase
■ Faigofie-o faagaioiga uma e faamaeaina i le vevela o le potu
■ Saosaoa -e mafai ona faamaeaina i le 30 minute
■ Saogalemu-e le mana'omia se reagent organic
■ maualuga mama -OD260/280≈1.8-2.1

Fa'aoga pusa

E talafeagai mo le aveeseina ma le faʻamamaina o le RNA atoa mai le tele o mea fou pe faʻaisa manu aano poʻo sela faʻaleaganuʻu.

Fa'atauga o oloa

■ Talosaga i lalo: fa'asologa muamua o le cDNA, RT-PCR, mole mole, Blot i Matu, ma isi.
■ Fa'ata'ita'iga: aano o manu, sela fa'atupuina
■ Fuafuaga: Tissue 10-20mg, Cells(2-5)×10⁶
■ Ole maualuga ole gafatia ole fusifusia ole DNA ole koluma fa'amamaina: 80 μg
■ Elution voluma: 50-200 μl

Fa'agasologa o galuega

Ata

Manu Aofa'i RNA Isolation Kit togafitia 20mg
Fa'ata'ita'iga o isumu fou, ave 5% fa'amama atoa RNA 1% agar

Glycogel electrophoresis
1: Spleen 2: Fatugao
3: Ate 4: Loto

Teuina ma le ola fa'asa

E mafai ona teu le pusa mo le 24 masina i le vevela o le potu (15-25 ℃) poʻo le 2-8 ℃ mo se taimi umi.E mafai ona teuina le Buffer RL1 i le 4 ℃ mo le 1 masina pe a uma ona fa'aopoopo le β-mercaptoethanol (filifiliga).

O lo'o iai le matou tagata fa'apitoa, mataalia e tu'uina atu auaunaga maualuga mo le matou fa'atau.Matou te mulimuli i taimi uma i le faʻatonuga o tagata faʻatau, faʻamatalaga-taulai mo Fale Faʻatau Saina CE Faʻamaonia Nucleic Acid 64 Well Plate DNA Rna Extraction Isolation Kit mo PCR Virus Rapid Diagnostic, Matou te faʻafeiloaia oe e te faʻatasi ma i matou i lenei ala o le fatuina o se pisinisi manuia ma lelei faʻatasi.
Falegaosi Fa'atauSaina Viral Nucleic Acid Isolation, Su'esu'ega PCR Taimi Moni, “Ia sili atu ona aulelei tamaitai” o la matou filosofia faatau."O le avea ma tagata fa'atau oloa fa'atuatuaina ma fa'atauaina fa'atau oloa" o le sini lea a le matou kamupani.Matou te faʻamaoni i vaega uma o la matou galuega.Matou te faʻafeiloaʻi ma le faʻamaoni uo e faʻatalanoa pisinisi ma amata galulue faʻatasi.Matou te faʻamoemoe e faʻatasi lima ma uo i pisinisi eseese e fausia ai se lumanaʻi manuia.