O le falegaosimea e fa'afaigofie ma vave le taimi moni PCR Kit ma High Rox+ Hotstart Taq DNA Polymerase Power Green Qpcr Mix
O a matou mea e masani ona faʻamaonia ma faʻatuatuaina e tagata faʻatau ma e mafai ona faʻataunuʻuina le faʻaauauina o suiga o le tamaoaiga ma agafesootai manaʻoga o Fale gaosi Faʻafaigofie ma Vave Real Time PCR Kit ma High Rox + Hotstart Taq DNA Polymerase Power Green Qpcr Mix, Talu ona faavaeina le fale gaosi oloa, ua matou tautino nei i le alualu i luma o oloa fou.A o faʻaaogaina le saoasaoa o agafesootai ma le tamaoaiga, o le a tatou faʻaauau pea ona faʻaauau le agaga o le "maualuga maualuga, lelei, faʻafouga, amio saʻo", ma faʻaauau pea i le faʻaogaina o le "faʻatagaga e amata i, tagata faʻatau muamua, sili ona lelei".O le a matou faia se ta'amilosaga umi ma le mata'ina i laulu ma a matou soa.
O a matou mea e masani ona iloa ma fa'atuatuaina e tagata fa'atau ma e mafai ona fa'ataunu'uina le fa'aauauina o suiga o mana'oga tau tamaoaiga ma agafesootaiSaina Taq DNA Polymerase ma Qpcr, Ua pasia le aitema e ala i le faʻamaoniaga faʻamaonia a le atunuʻu ma ua talia lelei i la tatou pisinisi autu.O la matou 'au fa'ainisinia fa'apitoa e masani ona sauni e tautuaina oe mo fa'atalanoaga ma manatu fa'aalia.Ua mafai fo'i ona matou tu'uina atu ia te oe fa'ata'ita'iga e leai se totogi e fetaui ma au fa'amatalaga.O taumafaiga lelei atonu o le a faia e tu'uina atu ia te oe auaunaga sili ona aoga ma fofo.E tatau ona e fiafia i la matou kamupani ma fofo, ia mautinoa e te faʻafesoʻotaʻi ma matou e ala i le lafoina mai o imeli pe valaʻau vave mai.Ina ia mafai ona iloa a matou fofo ma atinaʻe.a sili atu, e mafai ona e sau i le matou falegaosimea e matamata ai.O le a matou faʻafeiloaʻi i taimi uma malo mai le lalolagi atoa i la matou kamupani.o fausia pisinisi pisinisi.fa'atasi ma i tatou.Faamolemole lagona le saoloto e talanoa mai ia i matou mo le faʻalapotopotoga.nd matou te talitonu o le a matou faʻasoa faʻamatalaga sili ona lelei fefaʻatauaʻiga faʻatasi ma a matou tagata faʻatau uma.
Fa'amatalaga Pusa
O le 2 × Real PCR FaigofieTMMix-SYBR saunia e le Real Time PCR EasyTM-SYBR Green I kit o se faiga faʻapipiʻi fou lea e faʻaogaina ai le SYBR Green I mo taimi moni PCR faʻateleina tali, lea e mafai ona faʻaleleia atili ai le faʻapitoa o oloa ma le tali atu.O lo'o tu'uina atu le ROX e fai ma vali fa'atonutonu totonu.O le malosi o le fluorescence o lenei pusa e 3-5 taimi e sili atu i oloa tutusa, e mafai ona sili atu ona maaleale ma faʻaalia manino le faʻaogaina o DNA mamanu.
2 × Moni PCR FaigofieTMFa'afefiloi-SYBR o lo'o iai le Taq DNA Polymerase fa'apitoa a Foregene.Pe a faatusatusa i enzymes masani Taq, o loʻo i ai le lelei o le maualuga o le faʻaleleia lelei, malosi faʻapitoa faʻapitoa ma le maualalo o le mismatch rate.E mafai ona faʻaitiitia le faʻalauteleina le faʻapitoa ma faʻaleleia le saʻo o le PCR.
Fa'amatalaga
Taimi Moni PCR Easyᵀᴹ-SYBR Green I Kit | ||||
Fua'iga pusa(20μl faiga) | QP-01011 | QP-01012 | QP-01013 | QP-01014 |
200T | 500T | 1000T | 2000T | |
2× Moni PCR FaigofieTMFaafefiloi-SYBR | 1 ml × 2 | 1.7 ml × 3 | 1.7 ml × 6 | 1.7 ml × 12 |
50× ROX Fa'ailoga Fa'ailoga | 400 μl | 1 ml | 1 ml × 2 | 1 ml × 4 |
Dnase-Free ddH2O | 1.7 ml | 1.7 ml × 2 | 10 ml | 20 ml |
Faatonuga | 1 | 1 | 1 | 1 |
Fa'apitoa & itu lelei
■ Faigofie—2X PCR Mix e faʻaitiitia ai faʻataʻitaʻiga sese ma le taimi faʻatino
■ Fa'apitoa—fa'alelei fa'alelei ma le vevela-amata Taq enzyme e mafai ona taofia le fa'ateleina le fa'apitoa ma le fa'avaeina muamua o le dimer.
■ Ma'ale'ale maualuga—e mafai ona iloa ni kopi maualalo o le mamanu
■ Lelei felagolagomai—fesoasoani ma le tele o taimi moni meafaifaaili PCR quantitative
Fa'aoga pusa
su'esu'ega qPCR
Fa'agasologa o galuega
Ata fa'atusa
Teuina ma le ola fa'asa
O lenei pusa e tatau ona teu ese mai le malamalama ma e tatau ona teuina i le -20℃.Afai e faʻaaoga soo, e mafai foi ona teuina i le 4 ℃ mo sina taimi puupuu (10 aso).
O a matou mea e masani ona faʻamaonia ma faʻatuatuaina e tagata faʻatau ma e mafai ona faʻataunuʻuina le faʻaauauina o suiga o le tamaoaiga ma agafesootai manaʻoga o Fale gaosi Faʻafaigofie ma Vave Real Time PCR Kit ma High Rox + Hotstart Taq DNA Polymerase Power Green Qpcr Mix, Talu ona faavaeina le fale gaosi oloa, ua matou tautino nei i le alualu i luma o oloa fou.A o faʻaaogaina le saoasaoa o agafesootai ma le tamaoaiga, o le a tatou faʻaauau pea ona faʻaauau le agaga o le "maualuga maualuga, lelei, faʻafouga, amio saʻo", ma faʻaauau pea i le faʻaogaina o le "faʻatagaga e amata i, tagata faʻatau muamua, sili ona lelei".O le a matou faia se ta'amilosaga umi ma le mata'ina i laulu ma a matou soa.
Faiga falegaosimeaSaina Taq DNA Polymerase ma Qpcr, Ua pasia le aitema e ala i le faʻamaoniaga faʻamaonia a le atunuʻu ma ua talia lelei i la tatou pisinisi autu.O la matou 'au fa'ainisinia fa'apitoa e masani ona sauni e tautuaina oe mo fa'atalanoaga ma manatu fa'aalia.Ua mafai fo'i ona matou tu'uina atu ia te oe fa'ata'ita'iga e leai se totogi e fetaui ma au fa'amatalaga.O taumafaiga lelei atonu o le a faia e tu'uina atu ia te oe auaunaga sili ona aoga ma fofo.E tatau ona e fiafia i la matou kamupani ma fofo, ia mautinoa e te faʻafesoʻotaʻi ma matou e ala i le lafoina mai o imeli pe valaʻau vave mai.Ina ia mafai ona iloa a matou fofo ma atinaʻe.a sili atu, e mafai ona e sau i le matou falegaosimea e matamata ai.O le a matou faʻafeiloaʻi i taimi uma malo mai le lalolagi atoa i la matou kamupani.o fausia pisinisi pisinisi.fa'atasi ma i tatou.Faamolemole lagona le saoloto e talanoa mai ia i matou mo le faʻalapotopotoga.nd matou te talitonu o le a matou faʻasoa faʻamatalaga sili ona lelei fefaʻatauaʻiga faʻatasi ma a matou tagata faʻatau uma.