O lo matou pipii mau i le mataupu faavae o le "O le tulaga lelei e mafai ona avea ma olaga o lau faalapotopotoga, ma o le igoa e mafai ona avea ma agaga o ia" moaveese le RNA atoa, Faafetai mo le faʻaaluina o lou taimi aoga e te alu ai ia i matou ma nofo i luga mo le faia o se felagolagomai manaia faʻatasi ma oe.
O lo matou pipii mau i le mataupu faavae o le "O le tulaga lelei e mafai ona avea ma olaga o lau faalapotopotoga, ma o le igoa e mafai ona avea ma agaga o ia" moaveese le RNA atoa, O loʻo folafola mai e le matou kamupani: tau talafeagai, taimi puʻupuʻu gaosiga ma faʻamalieina pe a uma le faʻatau atu, matou te faʻafeiloaia foi oe e asiasi i la matou fale gaosimea i soo se taimi e te manaʻo ai.Maimau pe ana maua se pisinisi manaia ma umi fa'atasi!!!

Fa'amatalaga

50 Sauniuniga, 200 Sauniuniga

Vaega o pusa

Fa'apitoa & itu lelei

■ Leai se mea e popole ai e uiga i le faaleagaina o le RNA;ole faiga atoa ole RNase-Free
■ Aveese lelei le DNA-faʻaaogaina le DNA-Cleaning Column
■ Aveese le DNA e aunoa ma le faaopoopoina o le DNase
■ Faigofie-o faagaioiga uma e faamaeaina i le vevela o le potu
■ Saosaoa -e mafai ona faamaeaina i le 30 minute
■ Saogalemu-e le mana'omia se reagent organic
■ maualuga mama -OD260/280≈1.8-2.1

Fa'aoga pusa

E talafeagai mo le aveeseina ma le faʻamamaina o le RNA atoa mai le tele o mea fou pe faʻaisa manu aano poʻo sela faʻaleaganuʻu.

Fa'atauga o oloa

■ Talosaga i lalo: fa'asologa muamua o le cDNA, RT-PCR, mole mole, Blot i Matu, ma isi.
■ Fa'ata'ita'iga: aano o manu, sela fa'atupuina
■ Fuafuaga: Tissue 10-20mg, Cells(2-5)×10⁶
■ Ole maualuga ole gafatia ole fusifusia ole DNA ole koluma fa'amamaina: 80 μg
■ Elution voluma: 50-200 μl

Fa'agasologa o galuega

Ata

Manu Aofa'i RNA Isolation Kit togafitia 20mg
Fa'ata'ita'iga o isumu fou, ave 5% fa'amama atoa RNA 1% agar

Glycogel electrophoresis
1: Spleen 2: Fatugao
3: Ate 4: Loto

Teuina ma le ola fa'asa

E mafai ona teu le pusa mo le 24 masina i le vevela o le potu (15-25 ℃) poʻo le 2-8 ℃ mo se taimi umi.E mafai ona teuina le Buffer RL1 i le 4 ℃ mo le 1 masina pe a uma ona fa'aopoopo le β-mercaptoethanol (filifiliga).

O la matou mau e pipii atu i le mataupu faavae o le "Uiga e mafai ona avea ma ola o lau faʻalapotopotoga, ma o le igoa e mafai ona avea ma agaga o ia" mo le Tau Sili i Saina Taʻaloga ma Talosaga Faʻafomaʻi Faʻamalolo Ponaivi Paʻu ma Muscle Tissue L-Glutamine Amino Acid, Faʻafetai mo le faʻaaluina o lou taimi aoga e te alu ai ia i matou ma nofo i luga mo le faia o se mafutaga lelei ma oe.
Tau sili ona lelei i luga o Saina Food Additive, Athletic, Ua folafola mai e le matou kamupani: tau talafeagai, taimi puʻupuʻu ma faʻamalieina pe a uma ona faʻatau atu auaunaga, matou te faʻafeiloaia foi oe e asiasi i la matou fale gaosimea i soo se taimi e te manaʻo ai.Maimau pe ana maua se pisinisi manaia ma umi fa'atasi!!!