Ua matou tuuto atu i le ofoina atu faigofie, taimi-sefe ma tupe-sefe tasi-taofi le faatauina mai auaunaga mo tagata faatau moFa'afaigofie ona fa'aoga CELL TOTAL RNA ISOLATION KIT, Matou te fa'afeiloa'ia ma le fiafia soa pisinisi laiti mai so'o se ituaiga olaga, fa'amoemoe e fa'atūina pisinisi faauo ma felagolagoma'i fa'afeso'ota'i ma oe ma maua se fa'amoemoe manumalo.
Ua matou tuuto atu i le ofoina atu faigofie, taimi-sefe ma tupe-sefe tasi-taofi le faatauina mai auaunaga mo tagata faatau moFa'afaigofie ona fa'aoga CELL TOTAL RNA ISOLATION KIT, o loʻo matou tulimatai atu nei i le sili atu le galulue faʻatasi ma tagata faʻatau mai fafo e faʻavae i luga o faʻamanuiaga tutusa.O le a matou galulue ma le loto atoa e faaleleia a matou oloa ma auaunaga.Matou te folafola atu foi e galulue faʻatasi ma paʻaga pisinisi e siitia la matou galulue faʻatasi i se tulaga maualuga ma fefaʻasoaaʻi manuia.Faʻafeiloaʻi faʻafeiloaʻi oe e asiasi i la matou fale gaosi ma le faʻamaoni.

Fa'amatalaga

50 Sauniuniga, 200 Sauniuniga

Vaega o pusa

Fa'apitoa & itu lelei

■ O le faagasologa atoa e faagaoioia i le vevela potu (15-25 ℃), e aunoa ma le taele aisa ma le centrifugation maualalo vevela.
■ Ole pusa atoa ole RNase-Free, e leai se mea e popole ai ile faʻaleagaina ole RNA.
■ DNA-Cleaning Column o lo'o fusifusia fa'apitoa ai le DNA, ina ia mafai e le pusa ona aveese fa'ama'i genomic DNA e aunoa ma le fa'aopoopoina o le DNase fa'aopoopo.
■ Mauaina RNA maualuga: RNA-na'o Koluma ma fua fa'atatau e mafai ona fa'amama lelei le RNA.
■ Saosaoa vave: faigofie ona faagaoioia ma mafai ona faamaeaina i le 11 minute.
■ Saogalemu: E le mana'omia se mea fa'aola.
■ Tulaga maualuga: O le RNA faʻamamaina e maualuga le mama, e leai se polotini ma isi mea leaga, ma e mafai ona faʻafeiloaʻi isi suʻega mulimuli ane.

Fa'aoga pusa

E talafeagai mo le aveeseina ma le faʻamamaina o le RNA atoa mai sela faʻaleaganuʻu ile 96, 24, 12, ma le 6-well plates.

Fa'agasologa o galuega

Ata

O le agarose gel ata ma'a o Cell Aofa'i RNA Isolation Kit togafitia le numera eseese o lo'o i luga, 20μl volume elution, ave 2μl faamamaina atoa RNA 1%.

Teuina ma le ola fa'asa

E mafai ona teu le pusa mo le 12 masina i le vevela o le potu (15–25 ℃) ma le 2–8 ℃ mo se taimi umi (24 masina).

Buffer cRL1 e mafai ona teuina i le 4 ℃ mo le 1 masina ina ua uma ona faaopoopo 2-hydroxy-1-ethanethiol(filifiliga). Matou te tuuto atu i le ofoina atu faigofie, taimi-sefe ma tupe-sefe tasi-taofi le faatauina mai auaunaga a tagata faatau mo Hot sale China Robenidine Hydrochloride Premix Best Selling Feed Ingredient, Matou te faʻafeiloaʻi ma le faʻafeiloaʻi i uo laiti pisinisi ma le faʻamoemoe e faʻafeiloaʻi au pisinisi laiti ma le faʻamoemoe e faʻatautaia se olaga faauo. fa'amoemoega.
Fa'atauga vevela Saina Feed Additive, Feed Grade, o loʻo matou tulimatai atu nei i le sili atu le galulue faʻatasi ma tagata faʻatau mai fafo e faʻavae i luga o faʻamanuiaga tutusa.O le a matou galulue ma le loto atoa e faaleleia a matou oloa ma auaunaga.Matou te folafola atu foi e galulue faʻatasi ma paʻaga pisinisi e siitia la matou galulue faʻatasi i se tulaga maualuga ma fefaʻasoaaʻi manuia.Faʻafeiloaʻi faʻafeiloaʻi oe e asiasi i la matou fale gaosi ma le faʻamaoni.