Fa'aitiitiga fa'atauga Saina Metotia Rna Nucleic Acid Extraction Kit ma Purification Kit
O a matou aufaigaluega e masani lava i le agaga o le "faʻaauau pea le faʻaleleia ma le lelei", ma aʻo faʻaaogaina mea e sili ona lelei, sili ona lelei ma sili atu auaunaga pe a uma ona faʻatau atu, matou te taumafai e maua le talitonuga o tagata taʻitoʻatasi mo le Faʻatauga Siisii Saina Metotia Rna Nucleic Acid Extraction Kit ma le Purification Kit, e faʻafeiloaʻi ai tagata faʻatau ma meafaigaluega sili ma kamupani, ma faʻaauau le atinaʻeina o masini fou pisinisi o la matou kamupani.Matou te tulimatai atu i la outou galulue faatasi.
O a matou aufaigaluega e masani lava i le agaga o le "faʻaauau pea le faʻaleleia ma le lelei", ma aʻo faʻaaogaina mea e sili ona lelei maualuga, taua lelei ma sili atu le faʻatau atu auaunaga, matou te taumafai e maua uma le talitonuga o tagata faʻatau moSaina Nucleic Acid Extraction, Metotia Bead maneta, Matou te setiina se faiga faʻatonutonu lelei.O loʻo i ai a matou toe faʻafoʻi ma fefaʻatauaʻiga faiga faʻavae, ma e mafai ona e fesuiaʻi i totonu o le 7 aso talu ona maua wigs pe afai o loʻo i totonu o le nofoaga fou ma matou te tautuaina le toe faaleleia e aunoa ma se totogi mo a matou fofo.E tatau ona e lagona le saoloto e faʻafesoʻotaʻi i matou mo nisi faʻamatalaga ma o le a matou tuʻuina atu ia te oe le lisi o tau tauva i lena taimi.
Fa'amatalaga Pusa
50 Sauniuniga, 200 Sauniuniga
O lenei pusa o loʻo faʻaaogaina le koluma vili ma le fua faʻavae e fausia e la matou kamupani, lea e mafai ona maua mai ai le maualuga o le mama ma le maualuga o le RNA atoa mai meaola eseese o manu ma le maualuga maualuga. E maua ai se DNA-Cleaning Column lelei, lea e mafai ona faigofie ona vavae ese ma adsorb genomic DNA mai le supernatant ma le lysate tissue, faigofie ma sefe taimi;RNA-na'o le Column e mafai ona fusifusia lelei le RNA ma e mafai ona fa'agasolo fa'atasi ma se fua fa'apitoa Tele fa'ata'ita'iga.
O le faiga atoa e RNase-Free, ina ia le faʻaleagaina le RNA na maua mai;Buffer RW1, Buffer RW2 faʻapipiʻi fufulu faiga, ina ia maua le RNA e saʻoloto mai le polotini, DNA, ion, ma le faʻaleagaina o mea faʻalaʻau.
Vaega o pusa:
Puipui RL1 , Puipui RL2 |
Puipui RW1, Puipui RW2 |
RNase-Free ddH2O, Koluma Fa'amama DNA |
RNA-Na'o Koluma |
Fa'apitoa & itu lelei
■ Leai se mea e popole ai e uiga i le faaleagaina o le RNA;ole faiga atoa ole RNase-Free
■ Aveese lelei le DNA-faʻaaogaina le DNA-Cleaning Column
■ Aveese le DNA e aunoa ma le faaopoopoina o le DNase
■ Faigofie-o faagaioiga uma e faamaeaina i le vevela o le potu
■ Saosaoa -e mafai ona faamaeaina i le 30 minute
■ Saogalemu-e le mana'omia se reagent organic
■ maualuga mama -OD260/280≈1.8-2.1
Fa'aoga pusa
E talafeagai mo le aveeseina ma le faʻamamaina o le RNA atoa mai le tele o mea fou pe faʻaisa manu aano poʻo sela faʻaleaganuʻu.
Fa'atauga o oloa
■ Talosaga i lalo: fa'asologa muamua o le cDNA, RT-PCR, mole mole, Blot i Matu, ma isi.
■ Fa'ata'ita'iga: aano o manu, sela fa'atupuina
■ Fuafuaga: Tissue 10-20mg, Cells(2-5)×106
■ Ole maualuga ole gafatia ole fusifusia ole DNA ole koluma fa'amamaina: 80 μg
■ Elution voluma: 50-200 μl
Fa'agasologa o galuega
Ata
Manu Aofa'i RNA Isolation Kit togafitia 20mg
Fa'ata'ita'iga o isumu fou, ave 5% fa'amama atoa RNA 1% agar
Glycogel electrophoresis
1: Spleen 2: Fatugao
3: Ate 4: Loto
Teuina ma le ola fa'asa
E mafai ona teu le pusa mo le 24 masina i le vevela o le potu (15-25 ℃) poʻo le 2-8 ℃ mo se taimi umi.E mafai ona teuina le Buffer RL1 i le 4 ℃ mo le 1 masina pe a uma ona fa'aopoopo le β-mercaptoethanol (filifiliga).
O a matou aufaigaluega e masani lava i le agaga o le "faʻaauau pea le faʻaleleia ma le lelei", ma aʻo faʻaaogaina mea e sili ona lelei, sili ona lelei ma sili atu auaunaga pe a uma ona faʻatau atu, matou te taumafai e maua le talitonuga o tagata taʻitoʻatasi mo le Faʻatauga Siisii Saina Metotia Rna Nucleic Acid Extraction Kit ma le Purification Kit, e faʻafeiloaʻi ai tagata faʻatau ma meafaigaluega sili ma kamupani, ma faʻaauau le atinaʻeina o masini fou pisinisi o la matou kamupani.Matou te tulimatai atu i la outou galulue faatasi.
Fa'aitiitiga fa'aitiitigaSaina Nucleic Acid Extraction, Metotia Bead maneta, Matou te setiina se faiga faʻatonutonu lelei.O loʻo i ai a matou toe faʻafoʻi ma fefaʻatauaʻiga faiga faʻavae, ma e mafai ona e fesuiaʻi i totonu o le 7 aso talu ona maua wigs pe afai o loʻo i totonu o le nofoaga fou ma matou te tautuaina le toe faaleleia e aunoa ma se totogi mo a matou fofo.E tatau ona e lagona le saoloto e faʻafesoʻotaʻi i matou mo nisi faʻamatalaga ma o le a matou tuʻuina atu ia te oe le lisi o tau tauva i lena taimi.