Fa'aSaina Tomai Fa'apolofesa Fa'agata Fofo Fa'asaoina Fa'ama'i Lelei ma Rnase Inhibitors
Fa'amatalaga o pusa:
◮O se inhibitor RNase fou e maua mai i le isumu o lo'o fa'aalia i siama fa'ainisinia E. coli e fa'aaoga ai le fa'aogaina o le fa'aogaina o le gene.O le inhibitor e taofia ai le gaioiga a le RNase e ala i le le faʻatauvaʻa tuʻufaʻatasia ma le RNase 1: 1, ma puipuia ai.RNAamiosa'o , ma e mafai ona fa'alavelave leleio leRNase A, B, C gaoioiga, ae le o RNase T1, T2, H, ma isi; E mafai ona toe fa'aliliuina o le fusifusia o RNase Inhibitor ma RNase, ma le lavelave e mafai ona vavaeeseina e le urea ma le sulfhydryl reagents, ina ia toe faʻafouina le RNase ma le inhibitor e le mafai ona faʻaaogaina.
Faʻatasi ai ma le faʻatuatuaina o auala lelei, faʻamaumauga lelei ma le auʻaunaga lelei atoatoa, o le faʻasologa o fofo e gaosia e la matou atinaʻe e faʻatau atu i le tele o atunuʻu ma itulagi mo Chinese Professional Inactivated Efficient Virus Preservation Solution ma Rnase Inhibitors, Ma e mafai ona matou fesoasoani e suʻe soʻo se oloa o manaʻoga o tagata faʻatau.Ia mautinoa e tuʻuina atu le Auaunaga sili, le Tulaga Sili, Le Tiliva vave.
Fa'atasi ai ma auala lelei fa'atuatuaina, fa'amaumauga mata'ina ma le 'au'aunaga atoatoa mo tagata fa'atau, o le fa'asologa o fofo e gaosia e la tatou atina'e e fa'atau atu i le tele o atunu'u ma itulagi moFa'asao Fa'ama'i a Saina fa'atasi ai ma Fa'agata Rnase ma Reagent Fa'asaoina Fa'ama'i Fa'aletonu, Mo soʻo se tasi e naunau i soʻo se a matou oloa pe a uma ona e vaʻai i la matou lisi o oloa, faʻamolemole lagona le matua saoloto e faʻafesoʻotaʻi ma matou mo faʻamatalaga.E mafai ona e auina mai ia i matou imeli ma faʻafesoʻotaʻi matou mo faʻatalanoaga ma o le a matou tali atu ia te oe i se taimi vave e mafai ai.Afai e faigofie, e mafai ona e suʻeina le matou tuatusi i la matou upega tafaʻilagi ma sau i la matou pisinisi mo nisi faʻamatalaga sili atu o matou fofo e oe lava ia.Matou te sauni i taimi uma e fausia sootaga faʻalauteleina ma tumau ma soʻo se tagata faʻatau i totonu o matata faʻapitoa.
Fa'amatalaga
5ml, 10ml, 40ml
O le PCR Hero (Fa'atasi ma le Fa'a) Pusa fa'aaogā e aofia ai le tupulaga fou tulaga ese a Foregene o Taq—Foregene Taq Hero, dNTPs sili ona lelei, MgCl2, fa'afefe tali, fa'aleleia o tali PCR, fa'asili ma fa'amautu.O le 2 × PCR HeroTM Mix (lanu) faiga e maualuga atu le faapalepale i PCR inhibitors nai lo le PCR Mix masani faiga, ma e mafai ona faigofie ona taulimaina PCR amplification o mamanu lavelave eseese.O le faiga faʻapitoa faʻaalia ma le maualuga o le Taq Hero e faʻaalia ai le tali a le PCR e sili atu le faʻaleleia atili, faʻapitoa ma le maaleale.
Vaega o pusa
| 0.1U Taq Hero/μl |
| 400μM dNTPs |
| 20mM Tris-HCl(pH8.8) |
| 100mM KCl |
| 5mM MgCl2 |
| O isi fa'amautu ma fa'aleleia |
| DNA Loading Buffer |
Fa'apitoa & itu lelei
■ Faʻamaoni maualuga: 6 taimi nai lo le enzyme masani Taq;
■ Faʻateleina faʻavavevave faʻavavevave : o le saoasaoa faʻateleina o le 5-10sec / kb, lea e 6-12 taimi o le enzyme masani Taq;
■ E sili atu le fetuutuuna'i o mamanu: E mafai ona fa'atuputeleina fa'ata'ita'iga DNA lavelave eseese ma maualugaGC anotusi;
■ E sili atu le lelei o le fa'atuputeleina: ta'amilosaga fa'aola maualalo ifo nai lo le taq enzyme masani;
■ E sili atu le malosi o le faʻapalepale o le siosiomaga: tuʻu i le 37 ° C mo le vaiaso, faʻatumauina le sili atu i le 90% gaioiga;
■ E iai le 5′ →3′ DNA polymerase activity ma le 5′→3′ exonuclease activity, e aunoa ma le 3′ →5′ exonuclease activity.
■ O le pusa o loʻo muamua faʻafefiloi ma le Electrophoresis Loading Buffer, pe a maeʻa le PCR, e mafai ona uta saʻo le faʻataʻitaʻiga mo le electrophoresis;e faigofie ma faigofie le taotoga.
Fa'aoga pusa
PCR fa'ateleina o vaega DNA, fa'ailoga DNA, fa'asologa o DNA, PCR fa'atasi ma le si'usi'u
Teuina ma le ola fa'asao
E mafai ona teuina mo se taimi umi ile -20°C;mo le faʻaaogaina faifaipea, e mafai ona teuina i le 4 ° C mo sina taimi puupuu. Faatasi ai ma le faʻatuatuaina o auala lelei, faʻamaumauga lelei ma le auʻaunaga lelei atoatoa, o le faʻasologa o fofo e gaosia e la matou atinaʻe e faʻatau atu i le tele o atunuu ma itulagi mo Saina Faʻapolofesa Inactivated Efficient Virus Preservation Solution ma Rnase Inhibitors, Ma e mafai ona matou fesoasoani e suʻe soʻo se oloa o manaʻoga o tagata faʻatau.Ia mautinoa e tuʻuina atu le Auaunaga sili, le Tulaga Sili, Le Tiliva vave.
Faapolofesa SainaFa'asao Fa'ama'i a Saina fa'atasi ai ma Fa'agata Rnase ma Reagent Fa'asaoina Fa'ama'i Fa'aletonu, Mo soʻo se tasi e naunau i soʻo se a matou oloa pe a uma ona e vaʻai i la matou lisi o oloa, faʻamolemole lagona le matua saoloto e faʻafesoʻotaʻi ma matou mo faʻamatalaga.E mafai ona e auina mai ia i matou imeli ma faʻafesoʻotaʻi matou mo faʻatalanoaga ma o le a matou tali atu ia te oe i se taimi vave e mafai ai.Afai e faigofie, e mafai ona e suʻeina le matou tuatusi i la matou upega tafaʻilagi ma sau i la matou pisinisi mo nisi faʻamatalaga sili atu o matou fofo e oe lava ia.Matou te sauni i taimi uma e fausia sootaga faʻalauteleina ma tumau ma soʻo se tagata faʻatau i totonu o matata faʻapitoa.
