CDKN2A/CSP9 Su'esu'ega Lanu Lua
Fa'amatalaga o pusa:
O le fa'afefeteina i totonu o le nofoaga (FISH) e fa'avae i luga o le fa'avae o le fa'aopoopoina fa'atasi o fa'avae DNA, ma le va'aiga o fa'ailo fa'ameaalofa o su'esu'ega DNA fa'aigoa fa'amalama fa'atasi ai ma ona fa'asologa fa'asologa o le DNA i le nucleus cell i lalo ole microscope fluorescence.
Fa'amatalaga
O le fa'afefeteina i totonu o le nofoaga (FISH) e fa'avae i luga o le fa'avae o le fa'aopoopoina fa'atasi o fa'avae DNA, ma le va'aiga o fa'ailo fa'ameaalofa o su'esu'ega DNA fa'aigoa fa'amalama fa'atasi ai ma ona fa'asologa fa'asologa o le DNA i le nucleus cell i lalo ole microscope fluorescence.
Vaega o pusa
| Vaega | Fa'amatalaga | ||
| 5Su'ega | 10Su'ega | 20Su'ega | |
| CDKN2A/CSP9 Su'esu'ega Lanu Lua | 50μl | 100μl | 200μl |
Fa'aoga pusa
O lenei pusa o loʻo faʻaaogaina e suʻesuʻe lelei ai le faʻalavelave o le CDKN2A tagata i faʻataʻitaʻiga cytological poʻo faʻataʻitaʻiga o mea faʻapipiʻi paraffin in vitro.
Fa'ata'ita'iga mana'oga
- Tisu fa'ata'ita'iga
O fa'ata'ita'iga o mea'ai fa'apipi'i paraffin na fa'amauina i le 10% fa'apolopolo fa'apolopolo, ma o le mafiafia o le fasi i le va o le 3 ma le 5 µm.
- Fa'ata'ita'iga sela
Mo su'i ponaivi po'o fa'ata'ita'iga toto pito i lalo, e tatau ona teu i le 4°C mo le sili atu i le 24 itula, ma teu le sela tumau i le -20°C.
Teuina ma le ola fa'asao
Taofi ile -20℃±5℃ ile pogisa, aoga mo le 12 masina.Felauaiga i lalo ole 8 ℃.
