Toto RNA Tu'uesea Pusa
Fa'amatalaga o pusa:
Cat.Leai.RE-04011/04013
Mo le faamamaina atoa o le RNA mai le toto atoa 104 ≤ Selula Pa'epa'e ≤ 107
Vave fa'ate'a ma fa'amama RNA toto mai sela pa'epa'e.
-E leai se mea e popole ai e uiga i le faʻaleagaina o le RNA.Ole pusa atoa ole RNase-Free
-Fa'afaigofie-o fa'agaioiga uma e mae'a ile vevela ole potu
- Anapogi-e mafai ona faʻamaeʻaina le gaioiga i le 20 minute
-O le RNA maualuga: RNA-na'o le Koluma ma le fua fa'atatau e mafai ona fa'amama lelei le RNA
-Saogalemu-e leai se mea fa'aola fa'aoga e fa'aaogaina
-O le tele o faʻataʻitaʻiga gaioiga gafatia-e oʻo atu i le 200μl faʻataʻitaʻiga e mafai ona faʻatautaia i taimi taʻitasi.
- Tulaga maualuga-o le RNA faʻamamaina e sili ona mama, e leai se polotini ma isi mea leaga, ma e mafai ona faʻafeiloaʻi i le tele o faʻataʻitaʻiga faʻataʻitaʻiga.
Fa'amatalaga
O le pusa o loʻo faʻaaogaina le koluma vili ma le fua faʻavae na fausia e la matou kamupani, lea e mafai ona faʻaleleia lelei le RNA maualuga ma le maualuga o le RNA mai le toto atoa.O le pusa e maua ai le lysate toto mumu (Buffer RCL), lea e mafai ona vave ma lelei le lyse toto mumu ma taofi sela paʻepaʻe.O le DNA-Cleaning Colunm e mafai ona faigofie ona vavaeeseina le supernatant ma cell lysates ma faʻafefe ma aveese genomic DNA.O le taotoga e faigofie ma sefe taimi;O le RNA-na'o le Column e mafai ona fusia lelei le RNA, ma fa'atasi ai ma se fua fa'atatau, e mafai ona fa'agasolo le tele o fa'ata'ita'iga i le taimi e tasi.
O le polokalama atoa RNase-Free e le mafai ona fa'aleagaina le RNA ua maua mai;Fa'apolopolo RW1 ma le Buffer RW2 fa'amama fa'amama fa'amama e fa'asala ai le RNA maua mai polotini, DNA, ions ma mea fa'aleaganu'u filogia.
Pusa i totonu
| Toto Aofa'i RNA Isolation Kit | ||
| Fausia pusa | RE-04011 | RE-04013 |
| 50 taimi | 200 taimi | |
| Taofi RCL (10×) | 52.5 mL | 210ml |
| Taofi BRL1* | 30ml | 120ml |
| Puipui BRL2 | 18ml | 66ml |
| Puipui RW1* | 25ml | 100ml |
| Puipui RW2 | 24ml | 96ml |
| RNase-Free ddH2 O | 10ml | 40ml |
| RNA-na'o Koluma | 50 seti | 200 seti |
| DNA-Cleaning Koluma | 50 seti | 200 seti |
| tusi lesona | 1 kopi | 1 kopi |
Fa'apitoa & itu lelei
-E leai se mea e popole ai e uiga i le faʻaleagaina o le RNA.Ole pusa atoa ole RNase-Free.
-Fa'afaigofie-o fa'agaioiga uma e mae'a ile vevela ole potu.
- Anapogi-e mafai ona faʻamaeʻaina le gaioiga i le 20 minute.
-O le RNA maualuga: RNA-na'o le Koluma ma le fua fa'atatau e mafai ona fa'amama lelei le RNA.
-Saogalemu-e leai se mea fa'aola e fa'aaogaina.
-O le tele o faʻataʻitaʻiga gaioiga gafatia-e oʻo atu i le 200μl faʻataʻitaʻiga e mafai ona faʻatautaia i taimi taʻitasi.
- Tulaga maualuga-o le RNA faʻamamaina e sili ona mama, e leai se polotini ma isi mea leaga, ma e mafai ona faʻafeiloaʻi i le tele o faʻataʻitaʻiga faʻataʻitaʻiga.
Fa'asologa o pusa
Fa'aoga pusa:
E talafeagai mo le aveeseina ma le faʻamamaina o le RNA atoa mai le toto atoa mammalian.
Fa'agasologa o galuega
Tulaga teuina
Buffer RCL (10×) e tatau ona teuina i le 2-8 ℃;isi vaega o le pusa e mafai ona teuina i le vevela o le potu (15-25 ℃) i lalo o tulaga mago, ma e mafai ona teuina mo le 12 masina.E mafai ona teuina le Buffer BRL1 i le 4 ℃ mo le 1 masina pe a uma ona tuʻuina atu le β-mercaptoethanol (filifiliga) .
Fa'aaliga: Afai e teu ile vevela maualalo, ole vaifofo e faigofie ile timu.Ia mautinoa e tuʻu le vaifofo i totonu o le pusa i le vevela o le potu mo se vaitaimi aʻo leʻi faʻaaogaina.Afai e mana'omia, mua'i fa'avevela i totonu o le vai taele 37° C mo le 10 minute e fa'amuta ai le sua, ma fa'afefiloi lelei a'o le'i fa'aaogaina.
Ta'iala mo su'esu'ega fa'afitauli
The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail：Tech@foregene.com。
E leai se RNA e mafai ona maua mai pe maualalo le gaosiga o le nucleic acid
E masani ona tele mea e aʻafia ai le toe faʻaleleia lelei, e pei o le: faʻataʻitaʻiga RNA anotusi, auala o le faʻagaioiga, elution volume, etc.
Iloiloga o mafua'aga masani:
1. Ta'ele aisa po'o le maualalo o le vevela (4 ° C) centrifugation i le taimi o le taotoga.
Fautuaga: O le vevela o le potu (15-25 ° C) gaioiga, aua le ta'ele aisa ma le centrifuge maualalo.
2. Le lelei le teuina o faʻataʻitaʻiga poʻo le teuina o faʻataʻitaʻiga mo se taimi umi.
Fautuaga: Teu fa'ata'ita'iga ile -80°C po'o le fa'a'aisa ile fa'a'aisa sua, ma 'alofia le fa'aaogaina fa'a'aisa-liu;taumafai e faʻaaoga faʻataʻitaʻiga fou na aoina mo le faʻaaogaina o le RNA.
3. Le lava faʻataʻitaʻiga lysis
Fautuaga: Faʻamolemole faʻamautinoa o le faʻataʻitaʻiga ma le fofo galue (Linear Acrylamide) ua maeʻa faʻafefiloi ma faʻafefe mo le 10 min i le vevela o le potu (15-25 ° C)
4. Ua le sa'o le fa'aopoopoina o le eluent
Fautuaga: Ia mautinoa ua faaopoopo le RNase-Free ddH2O i le ogatotonu o le membrane o le koluma faʻamamaina.
5. Le talafeagai voluma o anhydrous ethanol i Buffer viRW2
Fautuaga: Fa'amolemole mulimuli i fa'atonuga, fa'aopoopo le voluma sa'o o le ethanol anhydrous ile Buffer viRW2 ma fa'afefiloi lelei a'o le'i fa'aogaina le pusa.
6. Fa'aoga fa'ata'ita'iga le talafeagai.
Fautuaga: 200μl o fa'ata'ita'iga ile 500μl ole Buffer viRL.O le tele o fa'ata'ita'iga voluma o le a fa'aitiitia ai le fua fa'atatau o le RNA.
7. le lelei o le elution volume po'o le le atoatoa o le elution.
Fautuaga: O le voluma eluent o le koluma faʻamamaina o le 30-50μl;afai e le faamalieina le aafiaga o le elution, e fautuaina e faʻaopoopo i ai le RNase-Free ddH muamua faʻavevela.2O ma faʻalautele le taimi tuʻu i le vevela o le potu, pei ole 5-10min
8. Fa'amama koluma ei ai ethanol toega ina ua uma ona fufulu i Buffer viRW2.
Fautuaga: Afai e tumau pea le ethanol pe a uma ona fufulu ile Buffer viRW2 ma centrifugation gaogao-tube mo le 2min, e mafai ona tuʻu le koluma faʻamamaina i le vevela o le potu mo le 5min pe a uma le centrifugation gaogao-tube e aveese atoa ai le ethanol totoe.
Le faʻaleagaina o mole RNA mama
O le lelei o le RNA faʻamamaina e fesoʻotaʻi ma mea taua e pei o le teuina o faʻataʻitaʻiga, faʻaleagaina o le RNase, ma le gaioiga.
Iloiloga o mafua'aga masani:
1. O faʻataʻitaʻiga na aoina e leʻi faʻasaoina i le taimi.
Fautuaga: Afai e le faʻaaogaina le faʻataʻitaʻiga i le taimi pe a maeʻa le aoina, faʻamolemole teu i le -80 ℃ poʻo le vai faʻamaʻi vave.Mo le su'eina o molela'au RNA, taumafai e fa'aoga fa'ata'ita'iga fou na aoina pe a mafai.
2. O fa'ata'ita'iga fa'aputuina sa fa'aaisa ma liusua fa'afia.
Fautuaga: Aloese mai le fa'aaisa ma fa'alili (ia le sili atu ma le tasi) i le taimi o le aoina ma le teuina o fa'ata'ita'iga, a leai o le a fa'aitiitia le fua o le nucleic acid.
3. Na fa'afeiloa'i le RNase i totonu o le potu tipitipi pe leai ni totini lima, matapulepule, ma isi mea na ofuina.
Fautuaga: Ole su'ega ole su'ega mole ole RNA e sili ona faia ile potu ta'i RNA, ma fa'amama le laulau fa'ata'ita'i a'o le'i faia le su'ega.Fa'aofu totigilima ma matapulepule i le taimi o le fa'ata'ita'iga e aloese ai mai le fa'aleagaina o le RNA e mafua mai i le fa'aofiina o le RNase.
4.O le reagent e faʻaleagaina e RNase i le taimi o le faʻaaogaina.
Fautuaga: Sui ile Viral RNA Isolation Kit mo su'ega fa'atatau.
5. O le RNase contamination o le centrifuge tubes, pipette tips, ma isi Fautuaga: Ia mautinoa o le centrifuge tubes, pipette tips, ma pipettes uma RNase-Free.
O molelaula RNA mama na a'afia i fa'ata'ita'iga i lalo
O mole RNA faʻamamaina e le koluma faʻamamaina o le a aʻafia ai faʻataʻitaʻiga i lalo pe afai e tele naua ion masima poʻo polotini, e pei ole: faʻaliliuga faʻaliliu, Northern Blot, ma isi.
1.O lo'o totoe ions masima i le eluted RNA molecules.
Fautuaga: Ia mautinoa ua faaopoopo le voluma sao o le ethanol anhydrous i le Buffer viRW2, ma fufulu le koluma faamama faalua e tusa ai ma le sao sao centrifugation i luga o faatonuga faagaioia; Afai o loʻo i ai pea ion masima o totoe, e mafai ona e faʻaopopo le Buffer viRW2 i le koluma faʻamamaina, ma tuʻu i le vevela o le potu mo le 5min.Ona faia lea o le centrifugation e aveese ai iona masima i se tulaga sili
2.O lo'o totoe le ethanol i le molelaula RNA eluted
Fautuaga: o le taimi lava e faʻamaonia ai o koluma faʻamamāina ua fufuluina e Buffer viRW2, fai le centrifugation gaogao-tube e tusa ai ma le saoasaoa centrifugal i luga o faatonuga faʻagaioiga.Afai o lo'o totoe pea le ethanol, e mafai ona tu'u mo le 5 minute i le vevela o le potu pe a mae'a le fa'amama gaogao e aveese ai le ethanol o totoe i le tele.
Tusi Taiala:
Viral RNA Isolation Kit Tusi Taiala
