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Fa'atonu PCR

O le a le Direct PCR

Direct PCR o le auala o le faʻalauteleina o DNA tuusaʻo maitoto, aano o manu,si'usi'u iole,sela, taliga isumu,separa i'a, fuamoa i'a,lau laau,fatupo'o isi ulua'i fa'ata'ita'iga o mea ola e aunoa ma le faia o le fa'aesea o DNA ma fa'amamāina.E mafai ona fa'aititia tele le taimi fa'ata'ita'i, ma le tau i fa'asologa fa'asolosolo ma galuega tetele.E maua ai fo'i se filifiliga sili atu pe a feagai ma lu'itau o le fa'ateleina o se vaega la'ititi o fa'ata'ita'iga lea e mafai ai e le la'asaga fa'amamāina ona ta'ita'i atu ai i fa'ata'ita'iga leiloa.

FOREGENE's PCR Mix e malosi le fa'alavelave fa'alavelave, ona o le malosi FOREGENE pateni Taq enzyme (pateni fa'atagaina: ZL20161034512.0 (Saina), EP3450560 (Europa), PCT/CN2017/082154 (US), pateni 6894926 (Iapani)).O lona Taq enzyme e fa'aogaina ai le DNA recombination technology e toe tu'ufa'atasia ai le DNA binding structure region o le archaeal nucleic acid binding protein ma le vevela-resistant Taq DNA polymerase e fausia ai se DNA fa'ata'ita'i ma fa'ateleina le fa'atasi.O se Taq fa'alelei vevela-amata o lo'o taofia ai galuega eseese o le DNA polymerase muamua. O lenei Taq enzyme e mafai ona fusifusia i DNA fa'ata'ita'i ma amata ai le fa'atupuina o le DNA i fa'ata'ita'iga maualalo fa'atonu ma si'osi'omaga lavelave.

For fa'ata'ita'iga,ite faigofie pei o le fa'aopoopoina o lausela,laau (lau laiti, a'a po'o fatu) po'o manu fa'ata'ita'i ilebuffer, ma faaopoopo i ai le tulaga esematai fefiloi mapatinofa'amuamua ile paipa PCR ma fa'agasolo ile va'a vevela.O le fa'aogaina o pusa e lelei tele mo su'esu'ega fa'ata'ita'iga lapopo'a lea e taua tele ai le fa'asaoina o le taimi.

O le PCR reaction system o loʻo i ai le enzyme UNG ma le dUTP ua filifilia e tusa ai ma faʻataʻitaʻiga manaʻoga, lea e mafai ona aloese mai le faʻaleagaina e mafua mai i oloa faʻalauteleina PCR.

asdada (5)
asdada (1)

Tulaga lelei oDPCR tuusao

 

 

Fa'atonu PCR

Auala masani

(RNA fa'amamāina + masani PCR)

Fa'aaogāga meafaitino

Fa'aaogāina o le tino

itiiti

Tele

Sauniuniga mamanu

Ituaiga meafaitino

Leai se olo e mana'omia

Omea fa'ata'ita'iga

Fa'agaioiga

Tulaga tasi-tulaga, 10min

fa'alavelave lavelave, 1h

Fulu

1-96

1-24

tali PCR

faiga tali

Fa'aopoopoina 2×PCR fa'afefiloi

Dntp, MgCl2,10×PCR Pa'u,Taq enzyme,

Atalosaga

01 Fa'ailoaina o meaola ninii fa'ama'i

02 Fa'ailoga fa'avasegaina, fa'ailoga fa'a-genotype

03 Multiplex PCR / SNP su'esu'ega / PCR-RFLP

04 Fa'asologa/cloning

Fa'asologa o Oloa--Cell Direct RT-qPCR faasologa

asdada (3)

Fa'asologa

Igoa Oloa

Fa'amatalaga

Numera Fa'amaumauga

Tulaga Teuteu

Fa'atonu RT-qPCR Fa'asologa

QuickEasyTMCell Direct RT-qPCR Kit–SYBR Green I

200T

DRT-01011

Vaega I 4℃, Vaega II -20℃

1000T

DRT-01012