Ole auala ole PCR ma le fa'aleagaina ole aerosol ole nucleic acid ile fale su'esu'e ole nucleic acid e pei o itu e lua ole tupe.E mafai ona tatou filifili e maua pe leai, ae e le mafai ona tatou filifili pe tatou te mananao i ai pe faaalu.

1. Su'esu'eina ole DNA aveese

Ina ia ausia le faʻaogaina o le faʻaogaina ole nucleic acid aerosol contamination, e manaʻomia muamua le suʻeina o mea e aveese ai DNA e mafai ona aveese ai nucleic acids i se tulaga vai.Aua e le tele ni DNA aveese e matua aoga.Mo le metotia fa'ata'ita'i, fa'amolemole va'ai i le: E le mafai ona avea le fa'aesea o le DNA ma se " tulimanu lilo" o le falesu'esu'e!

I lenei faʻataʻitaʻiga, 100 kopi / μL (CT e tusa ma le 31) o le ASFV plasmid ma le DNA remover faʻatusatusa e le PCR numera sa faʻafefiloi i voluma tutusa, ona faʻaalia lea i le vevela o le potu mo 10min, 20min ma 30min i le faasologa.Ina ua maeʻa le faʻaaogaina o le nucleic acid, na faʻataunuʻuina le qPCR amplification.Na fa'atusatusaina se fa'atonuga lelei fa'afefiloi ma le plasmid ma le vai.Talu ai e le'i mae'a le fa'ata'ita'iga i le taimi e tasi, e ono iai se va'aiga fa'apitoa i le va o taunu'uga, ae e le a'afia ai le fa'ai'uga o le su'ega.I le taimi nei, ua ou iloiloina 10 oloa faʻatau DNA faʻatau.Na'o Numera 1, Numera 6 ma Numera 8 oloa e mafai ona fa'aleagaina lelei le DNA plasmid i le tulaga vai.O isi oloa e toetoe lava a leai se aoga.

Fuafuaga 1 A'afiaga o le aveesea o le vaila'au nucleic ole DNA fa'atau oloa

2. Ole su'ega ole a'afiaga ole DNA ole fa'ama'i pipisi e masani ona fa'aogaina

1. Su'esu'eina o vailaau fa'ama'i masani

E tele ituaiga o vailaau fa'ama'i e masani ona fa'aogaina i totonu o fale su'esu'e: aldehydes, phenols, ava malosi, quaternary ammonium salts, peroxides, chlorine preparations, ma acids ma bases.O a'afiaga fa'amama o nei vaila'au i luga o meaola ninii ua mae'a fa'amaonia e su'esu'ega, ae Le'o lava fa'amatalaga fa'ata'ita'iga ile a'afiaga leaga ole nucleic acids.E manaʻomia e fale suʻesuʻe suʻega nucleic acid se vailaʻau faʻamaʻi e le gata ina lelei le faʻamaʻiina o meaola ninii, ae mafai foi ona faʻaleagaina le DNA.O faʻaiʻuga o suʻega e faʻaalia ai e naʻo le lua chlorine sauniuniga o le 84 disinfectant ma le trichloroisocyanuric acid ma le 1 M hydrochloric acid e fetaui ma manaʻoga.Talu ai o le 1 M hydrochloric acid e sili ona pala, e fautuaina ai le fa'aogaina o le chlorine e fai ma otaota lafoai i fale su'esu'e su'ega nucleic acid.Ae ui i lea, o sauniuniga o le chlorine e pala i uʻamea ma e le mafai ona faʻaaogaina mo le faʻamaʻiina o meafaifaʻaili ma meafaigaluega.

Fuafuaga 2 A'afiaga ole DNA ole 'ese'ese ole vaila'au fa'ama'i

2. Le fa'aitiitiga lelei o le fa'aogaina ole chlorine

O vaila'au fa'ama'i chlorine e malosi le fa'aleagaina o le DNA, ae ona o lo latou fa'aleagaina o le u'amea ma le ita, e mafai ona fa'aaoga e fa'amama ai le fola, fa'auma e le o ni u'amea, fa'asusu fa'asusu, fa'aau centrifuge ma isi mea su'ega.

E tusa ai ma le "New Coronary Pneumonia Epidemic Disinfection Technical Guidelines": O mea filogia (toto o ma'i, fa'alilolilo ma pua'i) avanoa chlorine 5g/L-10g/L chlorine-containing disinfectant;fola, puipui, ma mea i luga ole mea e fa'aoga ai le chlorine avanoa 1g/L Fa'ama'i chlorine: o lavalava, moega ma isi ie e fa'asusu muamua ile chlorine fa'ama'i ile 0.5g/L ole chlorine avanoa mo le 30 minute, ona fa'amama lea e pei ona masani ai.

Fuafuaga 3 A'afiaga ole DNA ole fa'aogaina ole vaila'au fa'ama'i chlorine

O faʻaiʻuga o suʻega e faʻaalia ai: pe a sili atu pe tutusa le faʻaogaina o le chlorine i le 1.2 g/L, e mafai e le vailaʻau faʻamaʻi chlorine ona faʻaleagaina atoa le 100 kopi / μL plasmid pe a faʻaaogaina mo le 5 minute.Pe a galue mo le 10 minute, 84 faʻamaʻi faʻamaʻi faʻatasi ai ma le faʻaogaina o le chlorine sili atu pe tutusa ma le 0.6 g / L e mafai ona faʻaleagaina atoa le 100 kopi / μL o plasmids.

3. Fa'ata'ita'iga fa'ate'aina o le ea ua fa'aleagaina ile nucleic acid aerosol

E fa'afefea ona fa'amamāina le ea ua fa'aleagaina e le nucleic acid aerosols?O le tele o mafaufauga o le faia lea o se siosiomaga e afaina ai le aerosol ona faʻamamaina lea, poʻo le faʻamamaina o nucleic acids pe a uma ona faʻaleagaina.Mo aʻu, o se faʻataʻitaʻiga e le mafai ona toe faia ma o taunuuga o le suʻega e le mafai ona suʻesuʻeina faʻatusatusaga e leai se uiga, o lea na ou nono mai ai ni auala e faʻamaʻi ai le ea faʻamaʻi.Va'ai "GB27948-2020 Manaoga Lautele mo Fa'ama'i Fa'ama'i" ma le "Fa'amatalaga Fa'amatalaga Fa'ainisinia"

1. Mea e su'e ai

1.1 DNA aveese: I lenei suʻega, e lua faʻatau faʻatau DNA aveese 6 ma 8 i le Laulau 1 na filifilia.

1.2 Suʻega plasmid: Mafaufau o le maualuga o le CT o le tele o mea faʻaleagaina falesuesue o le a maualuga atu nai lo le 30, o le faʻaogaina o le plasmid gene ASFV na faʻaaogaina i lenei taimi e tusa ma le 100 kopi / μL, ma le PCR CT fluorescent quantitative e tusa ma le 31.07.Fa'aopoopo le plasmid i le fuga fuga fuga ma fa'agogo le ea.

1.3 Su'ega meafaigaluega: O le Bilingkehan KVBOX tu'uaiga mea faigaluega, lea e lagolagoina ma le agalelei e Shenzhen Runlian Siosiomaga Tekonolosi Co., Ltd., e sili ona lelei le faatinoga o le disinfection i le hydrogen peroxyide disinfectant, ma e mafai ona fasiotia lelei spores ma isi microorganisms.

1.4 Su'ega avanoa: o se fa'amalama fa'afeiloa'i tapuni e tusa ma le 0.1 kupita mita.

2. Faiga su'ega

O le aofaʻi o vailaʻau faʻapipiʻi i luga o le masini faʻamaʻi e 10ml, ma o le faʻatonuga o le 100ml / m3, e sili atu le maualuga nai lo le "GB27948-2020 Manaoga Lautele mo Faʻamaʻi Ea": pe a faʻaaogaina le faʻamaʻiina o le aerosol, o le aofaʻi o faʻamaʻi e tatau ona ≤10 ml / m3