Talaaga
I tausaga talu ai nei, o vesicle extracellular (EVs) ua tosina mai ai le mafaufau o tagata e avea o se mea faigaluega faʻapitoa;peita'i, e le'i fa'ailoaina le a'afiaga fa'afoma'i o EV ile endometriosis.O le Endometriosis o se faʻamaʻi masani e le faʻaleagaina e afaina ai le 10-15% o tamaʻitaʻi o loʻo fananau mai tausaga, e mafua ai faʻailoga eseese, e mafua ai le faʻaitiitia o le lelei o le olaga ma se avega lautele.
Folasaga o Mataupu
I le aso 20 o Iulai, 2021, na lomia ai e le vaega suʻesuʻe a le Polofesa Wang Guoyun mai le Falemaʻi Qilu o le Iunivesite o Shandong se pepa suʻesuʻe e faʻaulutalaina "M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis" i Frontiers in immunology, lea na talanoaina ai le faʻatupuina o M1 macrophages.Le faʻaogaina o nanovesicles (NVs) i le togafitiga o le endometriosis.
O lenei tusiga o loʻo faʻaaogaina auala faʻaauau pea e saunia ai M1NVs, ma faʻaogaina auala faʻa-aganuʻu e suʻesuʻe ai suiga i le angiogenesis, femalagaiga, osofaʻiga ma isi faʻailoga o eutopic endometrial stromal cell (EM-ESCs) mai tagata mamaʻi e maua i le endometriosis.I le taimi lava e tasi, na faʻavaeina ai se faʻataʻitaʻiga o le isumu o le endometriosis, ma o isumu na togafitia i le PBS, MONVs poʻo le M1NVs, i le faasologa, e iloilo ai le aoga ma le saogalemu o le M1NV i le togafitiga o le endometriosis.
O faʻaiʻuga na faʻaalia ai o le in vitro M1NVs e mafai ona faʻalavelave tuusaʻo pe leai foi le femalagaiga ma osofaʻiga a EM-ESCs, ma faʻalavelave ai le angiogenesis.I le faʻataʻitaʻiga o isumu, M1NVs faʻalavelave le tupu mai o le endometriosis e ala i le M2 macrophage reprogramming e aunoa ma le faʻaleagaina o totoga.O lo'o fa'aalia ai e mafai e M1NVs ona fa'alavelave sa'o le tupu mai o le endometriosis, ma e mafai fo'i ona fa'alavelave e ala i le toe fa'afo'iina o le M2 ituaiga macrophages i le M1.O le mea lea, o le faʻaaogaina o M1NVs atonu o se auala fou mo togafitiga o le endometriosis.
Foregene Fesoasoani
I le suʻesuʻega, talu ai na saunia le M1NV e ala i le faʻaauauina o le M1 macrophages, o le tusiga na faʻaogaina le qRT-PCR e iloa ai mea faʻamaʻi faʻamaʻi ma M1 macrophage markers iNOS, TNF-a ma IL-6 mRNA i M1NV ma M1 macrophages.Fa'atelega o suiga.O faʻaiʻuga na faʻaalia ai o M1NVs o loʻo i ai le tele o faʻamaʻi faʻamaʻi mRNA ma M1 macrophage maka, e faʻaalia ai e mafai e M1NV ona faʻatumauina lelei uiga faʻaogaina o sela M1.O lenei metotia su'esu'e e fa'aogaina ai le QuickEasy Cell Direct RT-qPCR Kit-SYBR Green I o Foregene
Cell Direct RT-qPCR Kitfa'amatalaga

Tala fa'atatau:
 
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2. Su'esu'eina o le fa'aaliga o kenela o sela faigata e mafai ona fa'afaileleina e pei o sela muamua, sela fa'ato'aga, ma sela neura;
3. Su'esu'eina o le mRNA i fa'ata'ita'iga e pei o exosomes ma nanovesicles.
Vaega: