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Zebra Fish Direct PCR Pusa
O lenei ato e faʻaaoga ai le lysis buffer system e vave ona faʻamatuʻu ai le genomic DNA mai le zebrafish ma isi meaola iʻa o le sami, o siʻusiʻu poʻo iʻa fualaʻau fualaʻau mo tali a le PCR, o lea e talafeagai lelei mo le tele o suʻesuʻega o gafa. O le gaioiga o le faʻasaʻolotoina o le genomic DNA mai le lysis buffer ua maeʻa i totonu o le 10-30 minute i le 65 ° C. Leai ni isi gaioiga e pei ole porotini ma le aveʻesea ole RNA e manaʻomia, ma ole faʻasaʻogaina o DNA e mafai ona faʻaaogaina ose template mo le tali atu ole PCR.
2 × PCR EasyTM Mix o loʻo iai le faʻapalepale malosi ile faʻalavelave faʻafuaseʻi ole PCR, ma mafai ona faʻaaoga le lysate o le faʻataʻitaʻiga e faʻataʻitaʻia o se faʻataʻitaʻiga mo le lelei ma le faʻapitoa o le faʻalautelega. O lenei mea faʻaaoga e aofia ai le ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, tali faʻamau, PCR faʻaleleia ma faʻamautuina. Faʻaaoga faʻatasi ma le lysis buffer e mafai ona vave maua gofie ni faʻataʻitaʻiga, ma e i ai uiga o le maualuga malamalama, malosi faʻapitoa, ma lelei mautu.
D-Taq DNA polymerase o se DNA polymerase faʻapitoa fausia e Foregene mo tuusaʻo PCR tali atu. O le D-Taq DNA polymerase e malosi lona faʻapalepale i le tele o mea e faʻaaoga e le PCR, ma e mafai ona faʻateleina ai le aofaʻi o DNA i vaega eseese o faʻafitauli, ma o le saoasaoa o le saoasaoa e oʻo atu i le 2Kb / min, e fetaui lelei ma le Direct PCR.
