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01

IF:17.694

Lolomiina e: The First Affiliated Hospital, Sun Yat-sen University

Autu autu: Esophageal squamous cell carcinoma (ESCC) o le vaega autu o le kanesa o le esophageal.O le fa'ailoaina o fa'amoemoega fa'afoma'i lelei e taua tele mo togafitiga lelei ole ESCC.O nisi suʻesuʻega ua faʻaalia ai o le RNA mismodification e mafai ona faʻalauiloa le faʻamatalaga ma le faʻaliliuga o mRNA e fesoʻotaʻi ma le kanesa, ae o loʻo tumau pea le le manino o le masini mole.O lenei suʻesuʻega na faʻaalia ai o le tRNA m7G methyltransferase-related faʻalavelave faʻalavelave METTL1 ma le WDR4 na matua faʻateleina i le ESCC tissues ma saofagā i le le lelei o faʻamaʻi o le ESCC.Talu mai lena taimi, METTL1 ma WDR4 e fesoʻotaʻi ma suʻesuʻega tuʻituʻi i le ESCC faʻataʻitaʻiga mice ua faʻamaonia ai o le faʻaleleia atili o le gaioiga o le tRNA m7G methyltransferase e taʻitaʻia ai le faʻatonuina o le faʻaalia o nei polotini e lua, lea e faʻateleina ai le faʻateleina o sela ESCC.O lenei suʻesuʻega o loʻo faʻaalia ai le taua tele o le faʻaogaina o le tRNA m7G suiga i le ESCC ma faʻaalia ai o le tulimataʻiina o le METTL1 ma ona auala faʻailoga pito i lalo atonu o se faʻamoemoega faʻapitoa mo togafitiga ESCC.

oloa fesoasoani:

Isu Si'u Direct PCR Kit Foregene#TP-01333

So'oga muamua:

https://www.nature.com/articles/s41467-022-29125-7

02

IF:14.903

Lolomiina e: Su'esu'ega Fa'apitoa a le Setete o Su'esu'ega Tulaga Fa'aSaina, Inisetiute o Fa'asaienisi Fa'afomai Saina, Iunivesite o Macau

Anotusi autu:

Faatasi ai ma le atinaʻeina o tekinolosi faʻatekonolosi, ua avea le mRNA ma se vasega fou o vailaʻau faʻamaʻi.Ae ui i lea, o le ausiaina lelei o le tuʻuina atu o vailaʻau mRNA e tumau pea le luʻitau ona o le maaleale o le mRNA i RNases.O lenei suʻesuʻega o loʻo tuʻuina atu ai se polokalame faʻafeiloaʻi talafeagai o polyphenol-conjugated nanostructures e mafai ona faʻaleleia lelei le IL-10 mRNA i vivo.A maeʻa ona faʻaogaina lenei nanostructure e tuʻuina atu ai vailaʻau mRNA, o le IL-10 maualuga na faʻatonuina faʻapitoa, faʻamalosia lelei le faʻaalia o mea faʻaleagaina, faʻaleleia le mucosal toe faʻaleleia, puipuia o sela epithelial colonic mai apoptosis, ma faʻalavelaveina le dextran sodium sulfate-induced acute and chronic small.

oloa fesoasoani:

Manu Aofa'i RNA Isolation Kit Foregene#RE-03011

So'oga muamua:

https://www.sciencedirect.com/science/article/pii/S2211383522001551#:~:text=The%20polyphenol-assisted%20IL-10%20mRNA%20delivery%20system%20with%20self-protection,dextran%20sulfate%20tduce%20sulfate%20sulfate%20s fa'ata'ita'iga.%202.

03

AFAI: 12.499

Lolomiina e: Falesu'esu'e o Fa'ata'ita'iga Fa'ata'ita'i Fa'ama'i Manu, Kolisi o Vailaau Fa'afomai, Sichuan Agricultural University

Anotusi autu:

O le faʻamaʻi faʻamaʻi o le sperm o se mea taua e mafua ai le le maua o tama, o lea o se filifiliga talafeagai ma aoga e faʻavaeina faʻataʻitaʻiga manu manu e ala i le fetuunaiga o le antioxidant system i se faiga faʻatatau.Ae ui i lea, e le'i fa'amalamalamaina le fa'aogaina o masini fa'aantioxidant e fa'atatau i le cell germ cell.O lenei suʻesuʻega na faʻamaonia ai o le LanCL1 gene o se faʻamaʻi faʻamaʻi faʻamaʻi i totonu o sela siama tane, ma o lona faʻaaliga e faʻaitiitia pe fesoʻotaʻi ma le le maua o tama tane.O isumu tane e le maua i le LanCL1 gene e faʻaalia ai le faʻaleagaina o le sperm oxidative ma le faʻaleagaina o le fausiaina.I le faagasologa o le sperm differentiation o siama siama, o le taimi lava e tupu ai le faʻamaʻi faʻamaʻi, transcription factor-specific protein 1 (SP1) o le a faʻatonutonuina le faʻaaliga o le LanCL1 gene, ma faʻafefe ai le faʻamaʻiina o le sperm.O lenei suʻesuʻega o loʻo faʻaalia ai o le SP1-LanCL1 ala o loʻo i ai se sao taua i le faʻatonutonuina o le testicular homeostasis ma tama tane e faʻatalanoaina e le paleni redox, ma faʻaalia ai o isumu LanCL1-modified e iai ni faʻaoga faatosina e avea o se manu faʻataʻitaʻiga o tama tane.

Oloa Fesoasoani:

Isu Si'usi'u DNA Mini Kit Foregene#DE-05211

Uluai So'oga:

http://europepmc.org/article/MED/35469022

Pusa PCR sa'o Si'u Isu

◮E le manaʻomia le faʻamamaina ole DNA e alu ai le taimi ma taugata.

◮E laʻititi le manaʻoga faʻataʻitaʻiga, naʻo le ave o le fatu e tasi.

◮E leai ni togafitiga faʻapitoa e pei o le oloina ma le tuʻimomomoina e manaʻomia, ma e faigofie le taotoga.

◮O le faiga o le PCR sili ona lelei e mafai ai e le PCR ona maualuga le fa'apitoa ma malosi atu le fa'apalepale i PCR fa'alavelave fa'alavelave.

Manu Aofa'i RNA Isolation Kit

◮E leai se mea e popole ai i le faʻaleagaina o le RNA.Ole faiga atoa ole RNase-Free.

◮ Aveese lelei le DNA e faʻaaoga ai le DNA-Cleaning Column.

◮ Aveese le DNA e aunoa ma le fa'aopoopoina o le DNase.

◮Fa'afaigofie—o fa'agaioiga uma e mae'a ile vevela ole potu.

◮Saosaoa—e mafai ona fa'auma le ta'aloga ile 30 minute.

◮Saogalemu—leai se mea e fa'aogaina fa'aola.

◮Malaga maualuga—OD260/280≈1.8-2.1.

Pusa Mini DNA Si'usi'u Isu

◮Leai se fa'aleaga RNase:Ole DNA-Only Column na saunia e le pusa e mafai ai ona aveese le RNA mai le genomic DNA e aunoa ma le faʻaopoopoina o le RNase i le taimi o le suʻega, e puipuia ai le falesuesue mai le faʻaleagaina e le RNase ese.

◮Saosaoa vave:O le Foregene Protease e sili atu lona gaioioiga nai lo mea fa'apena fa'apena, ma fa'ato'a eliina fa'ata'ita'iga a le tino;e faigofie le taotoga, ma e mafai ona maeʻa le faʻaogaina o le DNA genomic i totonu ole 20-80 minute.

◮Faigofie:O le centrifugation e faia i le vevela o le potu, ma e leai se manaʻoga mo le 4 ° C maualalo le vevela centrifugation poʻo le ethanol precipitation o DNA.

◮Saogalemu:E le mana'omia le su'eina o reagent.

◮Tulaga maualuga:O le DNA genomic ua maua mai o loʻo i ai ni vaega tetele, leai se RNA, leai se RNase, ma e matua maualalo le ion content, lea e mafai ona fetaui ma manaʻoga o suʻega eseese.

◮Micro-elution faiga:E mafai ona faʻateleina le faʻaogaina o DNA genomic, lea e faigofie mo suʻesuʻega i lalo poʻo le faʻataʻitaʻiga.